Growth and Nitrate Uptake in Nannochloropsis gaditana and Tetraselmis chuii Cultures Grown in Sequential Batch Reactors
Open accessAbstract
This study aims to determine the maximum specific biomass growth rates of Nannochloropsis gaditana and Tetraselmis chuii grown in sequential batch reactors. The maximum specific growth rates of N. gaditana and T. chuii were found to be 0.23 ± 0.04 gVSS/gVSS∙d and 0.22 ± 0.05 gVSS/gVSS∙d respectively. Optical density measured at 550 nm (OD550) accurately predict volatile suspended solids (VSS) concentration in N. gaditana growth media but not in T. chuii growth media. We observed decoupling of nitrate uptake from cell growth for both microalgae as growth continued well beyond the point where concentrations of nitrate in the growth media fell below the detection limit. This nitrate decoupling highlights the challenges in current microalgae growth models as growth was independent of the extracellular dissolved nitrogen in the system.
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f/2 media and BG-11 differ by 20 times in the amount of nitrogen (-N).
“For example, f/2 media [130–132] and BG-11 [83, 133, 134] differ by 20 times in the amount of nitrogen (-N).”
From Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae (2023) · cites this paper as [132]
The Growth Rates of N. gaditana section states that the cultures were grown in f/2 medium, supporting the citation’s identification of that medium. The passages found for BG-11, nitrogen concentration, and the 20-fold figure do not establish a comparison between the two media; no page number is available in our copy. (LLM, conf=0.82)
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Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae
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“For example, f/2 media [130–132] and BG-11 [83, 133, 134] differ by 20 times in the amount of nitrogen (-”
Background
- Authors
- Rafay, R., Uratani, J. M., Hernandez, H. H., & Rodríguez, J.
- Affiliations
- Khalifa University of Science and Technology (AE)
- Corresponding author
- Rodríguez, Jorge
- Venue
- Frontiers in Marine Science
- Publisher
- Frontiers Media SA
- Published
- 2020-02-19
- Volume
- 7
- ISSN
- 2296-7745 (ISSN-L)
- OpenAlex
- W3006794915
- Cited by
- 24
- References
- 36
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 3 · 2023: 6 · 2022: 3 · 2021: 4 · 2020: 3
- FWCI
- 0.79
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Marine Science (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Funders
- Khalifa University of Science, Technology and Research (8434000305/EX2019–003)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal ecosystems
- Keywords
- Nannochloropsis gaditana, Tetraselmis chuii, microalgae growth, nitrate uptake, sequencing batch reactor, specific growth rate, biomass growth, optical density, volatile suspended solids, dissolved nitrogen, microalgae cultivation
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