Cited by 48Ruminant Nutrition and Digestive PhysiologyAnimal Nutrition and PhysiologyAnaerobic Digestion and Biogas Production

The structural and functional capacity of ruminal and cecal microbiota in growing cattle was unaffected by dietary supplementation of linseed oil and nitrate

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Popova, Milka, McGovern, Emily, McCabe, Matthew S., Martin, Cécile, Doreau, Michel, Arbre, Marie, Meale, Sarah J., Morgavi, Diego P. +1 more·2017·Frontiers in Microbiology

Abstract

Microorganisms in the digestive tract of ruminants differ in their functionality and ability to use feed constituents. While cecal microbiota play an important role in post-rumen fermentation of residual substrates undigested in the rumen, limited knowledge exists regarding its structure and function. In this trial we investigated the effect of dietary supplementation with linseed oil and nitrate on methane emissions and on the structure of ruminal and cecal microbiota of growing bulls. Animals were allocated to either a CTL (control) or LINNIT (CTL supplemented with 1.9% linseed and 1.0% nitrates) diet. Methane emissions were measured using the GreenFeed system. Microbial diversity was assessed using amplicon sequencing of microbial genomic DNA. Additionally, total RNA was extracted from ruminal contents and functional mcrA and mtt genes were targeted in amplicon sequencing approach to explore the diversity of functional gene expression in methanogens. LINNIT had no effect on methane yield (g/kg DMI) even though it decreased methane production by 9% (g/day; P<0.05). Methanobrevibacter- and Methanomassiliicoccaceae-related OTUs were more abundant in cecum (72% and 24%) compared to rumen (60% and 11%) irrespective of the diet (P<0.05). Feeding LINNIT reduced the relative abundance of Methanomassiliicoccaceae mcrA cDNA reads in the rumen. Principal component analysis revealed significant differences in taxonomic composition and abundance of bacterial communities between rumen and cecum. Treatment decreased the relative abundance of a few Ruminococcaceae genera, without affecting global bacterial community structure. Our research confirms a high level of heterogeneity in species composition of microbial consortia in the main gastrointestinal compartments where feed is fermented in ruminants. There was a parallel between the lack of effect of LINNIT on ruminal and cecal microbial community structure and functions on one side and methane emission changes on the other. These results suggest that the sequencing strategy used here to study microbial diversity and function accurately reflected the absence of effect on methane phenotypes in bulls treated with linseed plus nitrate.

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  • From the ileum onwards, including caecum, colon and feces, favorable fermentation conditions are present again and microbial density and phylogenetic diversity increase to a level comparable to that of the rumen.

    “From the ileum onwards, including caecum, colon and feces, favorable fermentation conditions are present again and microbial density and phylogenetic diversity increase to a level comparable to that of the rumen (Frey et al., 2010; De Oliveira et al., 2013; Popova et al., 2017; He et al., 2018; Yeoman et al., 2018).”

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Authors
Popova, M., McGovern, E., McCabe, M. S., Martin, C., Doreau, M., Arbre, M., Meale, S. J., Morgavi, D. P., & Waters, S. M.
Affiliations
  • Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
  • Teagasc - The Irish Agriculture and Food Development Authority (IE)
Corresponding author
Popova, Milka
Venue
Frontiers in Microbiology
Published
2017-05-24
Volume
8
Issue
937
Pages
937-937
ISSN
1664-302X (ISSN-L)
OpenAlex
W2617357390
Cited by
48
References
57
Citations by year
2026: 5 · 2025: 4 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 5 · 2020: 5 · 2019: 7 · 2018: 10 · 2017: 2
FWCI
11.42
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Frontiers in Microbiology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Arrow@dit (Dublin Institute of Technology) (acceptedVersion)
  • T-Stór (Teagasc) (acceptedVersion, cc-by-nc-sa)
  • Arrow@dit (Dublin Institute of Technology) (submittedVersion, cc-by-nc-sa)
  • HAL (submittedVersion, cc-by-sa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • T-Stór (Teagasc) (submittedVersion, cc-by-nc-sa)
Funders
  • Ministère de l'Education Nationale, de l'Enseignement Supérieur et de la Recherche
  • Institut National de la Recherche Agronomique
  • DSM Nutritional Products
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
Keywords
rumen microbiota, cecal microbiota, linseed oil, nitrate supplementation, methane emissions, growing bulls, methanogenic archaea, mcrA gene, Methanobrevibacter, Ruminococcaceae, microbial community structure, ruminant feed additives

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