The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Mechanisms and Regulation of Extracellular DNA Release and Its Biological Roles in Microbial Communities
Open accessAbstract
The capacity to release genetic material into the extracellular medium has been reported in cultures of numerous species of bacteria, archaea, and fungi, and also in the context of multicellular microbial communities such as biofilms. Moreover, extracellular DNA (eDNA) of microbial origin is widespread in natural aquatic and terrestrial environments. Different specific mechanisms are involved in eDNA release, such as autolysis and active secretion, as well as through its association with membrane vesicles. It is noteworthy that in microorganisms, in which DNA release has been studied in detail, the production of eDNA is coordinated by the population when it reaches a certain cell density, and is induced in a subpopulation in response to the accumulation of quorum sensing signals. Interestingly, in several bacteria there is also a relationship between eDNA release and the development of natural competence (the ability to take up DNA from the environment), which is also controlled by quorum sensing. Then, what is the biological function of eDNA? A common biological role has not been proposed, since different functions have been reported depending on the microorganism. However, it seems to be important in biofilm formation, can be used as a nutrient source, and could be involved in DNA damage repair and gene transfer. This review covers several aspects of eDNA research: (i) its occurrence and distribution in natural environments, (ii) the mechanisms and regulation of its release in cultured microorganisms, and (iii) its biological roles. In addition, we propose that eDNA release could be considered a social behavior, based on its quorum sensing-dependent regulation and on the described functions of eDNA in the context of microbial communities.
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Cites
The complete genome sequence of the gram-positive bacterium Bacillus subtilis.
Kunst, F., Ogasawara, N., Moszer, I., Albertini, A. M., Alloni, G., Azevedo, V., Bertero, M. G., Bessières, P., Bolotin, A., Borchert, S., et al. (1997) · Nature · doi:10.1038/36786 · Cited by 3,679
Pseudomonas aeruginosa Displays Multiple Phenotypes during Development as a Biofilm
Sauer, K., Camper, A. K., Ehrlich, G. D., Costerton, J. W., & Davies, D. G. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.4.1140-1154.2002 · Cited by 1,691 · cited 1×
“In this sense, although several mechanisms were described for the eDNA release in planktonic cultures, some of them were not found or were different in biofilms (Sauer et al., 2002; Greiner et al., 2005; Allesen-Holm et al., 2006; Kim and Lee, 2016).”
BackgroundExplosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms
Turnbull, L., Toyofuku, M., Hynen, A. L., Kurosawa, M., Pessi, G., Petty, N. K., Osvath, S. R., Cárcamo-Oyarce, G., Gloag, E. S., Shimoni, R., et al. (2016) · Nature Communications · doi:10.1038/ncomms11220 · Cited by 709
Role of extracellular DNA in initial bacterial adhesion and surface aggregation
Das, T., Sharma, P. K., Busscher, H. J., van der Mei, H. C., & Krom, B. P. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.03119-09 · Cited by 326
Thomas, V. C., Thurlow, L. R., Boyle, D., & Hancock, L. E. (2008) · Journal of Bacteriology · doi:10.1128/jb.00314-08 · Cited by 302
Recent insights into Candida albicans biofilm resistance mechanisms
Mathé, L., & Van Dijck, P. (2013) · Current Genetics · doi:10.1007/s00294-013-0400-3 · Cited by 271
Beta toxin catalyzes formation of nucleoprotein matrix in staphylococcal biofilms
Huseby, M. J., Kruse, A. C., Digre, J., Kohler, P. L., Vocke, J. A., Mann, E. E., Bayles, K. W., Bohach, G. A., Schlievert, P. M., Ohlendorf, D. H., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0911032107 · Cited by 199
Thomas, V. C., Hiromasa, Y., Harms, N., Thurlow, L., Tomich, J., & Hancock, L. E. (2009) · Molecular Microbiology · doi:10.1111/j.1365-2958.2009.06703.x · Cited by 197
Das, T., Sharma, P. K., Krom, B. P., van der Mei, H. C., & Busscher, H. J. (2011) · Langmuir · doi:10.1021/la202013m · Cited by 98
Streptococcus gordonii comCDE (competence) operon modulates biofilm formation with Candida albicans
Jack, A. A., Daniels, D. E., Jepson, M. A., Vickerman, M. M., Lamont, R. J., Jenkinson, H. F., & Nobbs, A. H. (2015) · Microbiology · doi:10.1099/mic.0.000010 · Cited by 88
Cited by
The dental plaque biofilm matrix
Jakubovics, N. S., Goodman, S. D., Mashburn‐Warren, L., Stafford, G. P., & Cieplik, F. (2021) · Periodontology 2000 · doi:10.1111/prd.12361 · Cited by 488
Biofilms: Architecture, Resistance, Quorum Sensing and Control Mechanisms
Saxena, P., Joshi, Y., Rawat, K., & Bisht, R. (2019) · Indian Journal of Microbiology · doi:10.1007/s12088-018-0757-6 · Cited by 216
Devaraj, A., Buzzo, J. R., Mashburn-Warren, L., Gloag, E. S., Novotny, L. A., Stoodley, P., Bakaletz, L. O., & Goodman, S. D. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1909017116 · Cited by 133
Surfactin Facilitates Horizontal Gene Transfer in Bacillus subtilis
Danevčič, T., Dragoš, A., Spacapan, M., Stefanic, P., Dogsa, I., & Mandic-Mulec, I. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.657407 · Cited by 35
Clark, R. R., Judd, J., Lasek-Nesselquist, E., Montgomery, S. A., Hoffmann, J. G., Derbyshire, K. M., & Gray, T. A. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1804227115 · Cited by 29
Kadriu, E., Qin, S., Prezioso, S. M., & Christendat, D. (2026) · Microbiological Research · doi:10.1016/j.micres.2025.128382 · Cited by 2
Metadata
- Authors
- Ibáñez de Aldecoa, A. L., Zafra, O., & González-Pastor, J. E.
- Venue
- Frontiers in Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2017-07-26
- Volume
- 8
- Pages
- 1390-1390
- ISSN
- 1664-302X (ISSN-L)
- OpenAlex
- W2738027776
- Cited by
- 310
- References
- 10
- FWCI
- 8.25
- Type
- journal article
- Language
- en
- Open access
- gold
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