Streptococcus gordonii comCDE (competence) operon modulates biofilm formation with Candida albicans
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Candida albicans is a pleiomorphic fungus that forms mixed species biofilms with Streptococcus gordonii, an early colonizer of oral cavity surfaces. Activation of quorum sensing (QS; intercellular signalling) promotes monospecies biofilm development by these micro-organisms, but the role of QS in mixed species communities is not understood. The comCDE genes in S. gordonii encode a sensor-regulator system (ComDE), which is activated by the comC gene product (CSP, competence stimulating peptide) and modulates expression of QS-regulated genes. Dual species biofilms of S. gordonii ΔcomCDE or ΔcomC mutants with C. albicans showed increased biomass compared to biofilms of S. gordonii DL1 wild-type with C. albicans. The ΔcomCDE mutant dual species biofilms in particular contained more extracellular DNA (eDNA), and could be dispersed with DNase I or protease treatment. Exogenous CSP complemented the S. gordonii ΔcomC transformation deficiency, as well as the ΔcomC-C. albicans biofilm phenotype. Purified CSP did not affect C. albicans hyphal filament formation but inhibited monospecies biofilm formation by C. albicans. The results suggest that the S. gordonii comCDE QS-system modulates the production of eDNA and the incorporation of C. albicans into dual species biofilms.
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Several studies have investigated dual-species biofilms comprising Candida albicans and a bacterium commonly isolated from denture stomatitis, periodontitis, or dental caries, such as Streptococcus mutans, Streptococcus gordonii, Actinomyces viscosus, or Fusobacterium species.
“Several studies have investigated such dual-species biofilms comprising C. albicans and a bacterium commonly isolated from denture stomatitis, periodontitis, or dental caries, such as Streptococcus mutans, Streptococcus gordonii, Actinomyces viscosus, or Fusobacterium species (12, 81, 82).”
From <i>Candida albicans</i> Biofilms and Human Disease (2015) · cites this paper as [81]
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- Authors
- Jack, A. A., Daniels, D. E., Jepson, M. A., Vickerman, M. M., Lamont, R. J., Jenkinson, H. F., & Nobbs, A. H.
- Affiliations
- University of Bristol (GB)
- University at Buffalo, State University of New York (US)
- University of Louisville (US)
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 2015-02-01
- Volume
- 161
- Issue
- 2
- Pages
- 411-421
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- PMC
- PMC4314648
- OpenAlex
- W2022415795
- Cited by
- 88
- References
- 55
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 4 · 2023: 8 · 2022: 11 · 2021: 7 · 2020: 7 · 2019: 10 · 2018: 8 · 2017: 10 · 2016: 8 · 2015: 8
- FWCI
- 3.55
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Bristol Research (University of Bristol) (publishedVersion)
- Explore Bristol Research (submittedVersion)
- Funders
- University of Bristol
- National Institutes of Health
- National Institute of Dental and Craniofacial Research (DE016690, R01DE016690)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Keywords
- Streptococcus gordonii, Candida albicans, competence-stimulating peptide, quorum sensing, dual-species biofilms, extracellular DNA, biofilm dispersal, dental plaque, hyphal development
- MeSH terms
- Bacterial Proteins, Candida albicans, Candidiasis, DNA, Bacterial, Humans, Operon, Streptococcal Infections, Biofilms, Quorum Sensing, Streptococcus gordonii
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