Direct cell–cell contact activates SigM to express the ESX-4 secretion system in Mycobacterium smegmatis
Open accessAbstract
Significance: A conjugation model of mycobacterial interaction recently revealed that intercellular communication occurs between donors and recipients. This communication links two ESAT-6 (ESX) (type VII) secretion systems that are both required for conjugation. Functionally distinct ESX secretion systems are found in all mycobacteria, and they serve important virulence functions in pathogenic mycobacteria. We demonstrate that SigM, an extracytoplasmic transcription factor, activates ESX-4. Direct donor-recipient cell contact triggers the recipient cell to release membrane-sequestered SigM, which rapidly induces an ESX-4-focused regulon. The conservation of SigM and ESX-4 throughout mycobacteria suggests that this interaction-response network is intact and active in pathogens. Contact-dependent responses similar to those identified in our model system may therefore also mediate communal processes within infectious mycobacterial populations.
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SigM, an extracytoplasmic function σ factor, is an activator of ESX-4 expression and necessary for DCT in the recipient strain of M. smegmatis.
“Moreover, it was shown that SigM, an extracytoplasmic function σ factor, is an activator of ESX-4 expression and necessary for DCT in the recipient strain of M. smegmatis (54).”
From ESX/Type VII Secretion Systems—An Important Way Out for Mycobacterial Proteins (2019) · cites this paper as [54]
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Self-citation
- Authors
- Clark, R. R., Judd, J., Lasek-Nesselquist, E., Montgomery, S. A., Hoffmann, J. G., Derbyshire, K. M., & Gray, T. A.
- Affiliations
- New York State Department of Health (US)
- Division of Genetics, Wadsworth Center, New York State Department of Health, Albany, NY 12208;
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2018-06-25
- Volume
- 115
- Issue
- 28
- Pages
- E6595-E6603
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC6048512
- OpenAlex
- W2811470307
- Cited by
- 29
- References
- 40
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 3 · 2023: 4 · 2022: 2 · 2021: 4 · 2020: 4 · 2019: 6 · 2018: 1
- FWCI
- 2.30
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- NSF | BIO | Division of Molecular and Cellular Biosciences (MCB_1614178)
- HHS | NIH | National Institute of Allergy and Infectious Diseases (1R01AI09719101A1, 5R21AI119427-02)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Keywords
- sIgM, Mycobacterium smegmatis, cell-cell contact, conjugation, cell surface signaling, transconjugation
- MeSH terms
- Type IV Secretion Systems, Type VII Secretion Systems, Bacterial Proteins, Conjugation, Genetic, Transcription Factors, Gene Expression Regulation, Bacterial, Mycobacterium smegmatis, Gene Regulatory Networks
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