Cited by 29Bacterial Genetics and BiotechnologyTuberculosis Research and EpidemiologyMycobacterium research and diagnosis

Direct cell–cell contact activates SigM to express the ESX-4 secretion system in Mycobacterium smegmatis

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Clark, Ryan R., Judd, Julius, Lasek-Nesselquist, Erica, Montgomery, Sarah A., Hoffmann, Jennifer G., Derbyshire, Keith M., Gray, Todd A.·2018·Proceedings of the National Academy of Sciences

Abstract

Significance: A conjugation model of mycobacterial interaction recently revealed that intercellular communication occurs between donors and recipients. This communication links two ESAT-6 (ESX) (type VII) secretion systems that are both required for conjugation. Functionally distinct ESX secretion systems are found in all mycobacteria, and they serve important virulence functions in pathogenic mycobacteria. We demonstrate that SigM, an extracytoplasmic transcription factor, activates ESX-4. Direct donor-recipient cell contact triggers the recipient cell to release membrane-sequestered SigM, which rapidly induces an ESX-4-focused regulon. The conservation of SigM and ESX-4 throughout mycobacteria suggests that this interaction-response network is intact and active in pathogens. Contact-dependent responses similar to those identified in our model system may therefore also mediate communal processes within infectious mycobacterial populations.

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Crossref · 10.1073/pnas.1804227115

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  • SigM, an extracytoplasmic function σ factor, is an activator of ESX-4 expression and necessary for DCT in the recipient strain of M. smegmatis.

    “Moreover, it was shown that SigM, an extracytoplasmic function σ factor, is an activator of ESX-4 expression and necessary for DCT in the recipient strain of M. smegmatis (54).”

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Authors
Clark, R. R., Judd, J., Lasek-Nesselquist, E., Montgomery, S. A., Hoffmann, J. G., Derbyshire, K. M., & Gray, T. A.
Affiliations
  • New York State Department of Health (US)
  • Division of Genetics, Wadsworth Center, New York State Department of Health, Albany, NY 12208;
Venue
Proceedings of the National Academy of Sciences
Published
2018-06-25
Volume
115
Issue
28
Pages
E6595-E6603
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2811470307
Cited by
29
References
40
Citations by year
2026: 2 · 2025: 3 · 2024: 3 · 2023: 4 · 2022: 2 · 2021: 4 · 2020: 4 · 2019: 6 · 2018: 1
FWCI
2.30
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • NSF | BIO | Division of Molecular and Cellular Biosciences (MCB_1614178)
  • HHS | NIH | National Institute of Allergy and Infectious Diseases (1R01AI09719101A1, 5R21AI119427-02)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
  • Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
Keywords
sIgM, Mycobacterium smegmatis, cell-cell contact, conjugation, cell surface signaling, transconjugation
MeSH terms
Type IV Secretion Systems, Type VII Secretion Systems, Bacterial Proteins, Conjugation, Genetic, Transcription Factors, Gene Expression Regulation, Bacterial, Mycobacterium smegmatis, Gene Regulatory Networks

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