The vaginal community state types microbiome-immune network as key factor for bacterial vaginosis and aerobic vaginitis
Open accessAbstract
Regarding bacterial vaginosis (BV), the relevance of the vaginal microbiota to the women’s health fulfils a key role but knowledge gaps regarding aerobic vaginitis (AV) exist. This study aims to characterize vaginal microbiome and its relationship with the local immune mediators providing an opportunity to define the link between vaginal commensal microorganisms and opportunistic pathogens in the relation of a given vaginal community state type (CST). A total of 90 vaginal samples from Caucasian asymptomatic women of reproductive age (18y–40y) attending the yearly examination and not reporting any vaginal complaints were retrospectively evaluated for microbiome assessment and immune factors dosage. The samples were tested by the Ion Torrent PGM and the Luminex Bio-Plex technologies for the analysis of microbiome and immune factors, respectively. In our study, the CSTs classification together with the local immune response profiling represented a good predictive indicator of the vaginal health suggesting that the predominance of a specific Lactobacillus and its relative abundance are pivotal elements to maintain a physiologic status. Vaginal colonization from Bifidobacterium may absolve a protective role similar to that of Lactobacillus, corresponding to a new identified CST, although studies are needed to better clarify its clinical significance. Moreover, within each CST, a different pattern of inflammation is activated and orchestrated both by the dominant Lactobacillus spp. and by specific non-lactobacillus bacteria, and can give insights into the pathogenic mechanisms. In conclusion, this study contributes to the characterization of vaginal dysbiosis, reshaping this concept by taking into consideration the CST profiling, local immune marker, and immune-microbial network.
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Vaginal dysbiosis consists of prolonged deviation from low-diversity, Lactobacillus-dominated microbiome; bacterial vaginosis (BV) is the most common type.
“Vaginal dysbiosis consists of a prolonged deviation from a low-diversity, Lactobacillus (sensu lato) dominated microbiome; bacterial vaginosis (BV), an anaerobic polymicrobial disease, associated with subclinical vaginal inflammation, is the most common type (De Seta et al., 2019).”
From Viral Infections, the Microbiome, and Probiotics (2021) · cites this paper as De Seta et al., 2019
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QIIME allows analysis of high-throughput community sequencing data
Caporaso, J. G., Kuczynski, J., Stombaugh, J., Bittinger, K., Bushman, F. D., Costello, E. K., Fierer, N., Peña, A. G., Goodrich, J. K., Gordon, J. I., et al. (2010) · Nature Methods · doi:10.1038/nmeth.f.303 · Cited by 34,887 · see citing works
Vaginal microbiome of reproductive-age women
Ravel, J., Gajer, P., Abdo, Z., Schneider, G. M., Koenig, S. S. K., McCulle, S. L., Karlebach, S., Gorle, R., Russell, J., Tacket, C. O., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1002611107 · Cited by 4,254 · see citing works
Molecular Identification of Bacteria Associated with Bacterial Vaginosis
Fredricks, D. N., Fiedler, T. L., & Marrazzo, J. M. (2005) · New England Journal of Medicine · doi:10.1056/nejmoa043802 · Cited by 1,303 · see citing works
Martin, H., Richardson, B. A., Nyange, P. M., Lavreys, L., Hillier, S. L., Chohan, B. H., Mandaliya, K. N., Ndinya‐Achola, J. O., Bwayo, J., & Kreiss, J. K. (1999) · The Journal of Infectious Diseases · doi:10.1086/315127 · Cited by 891 · see citing works
Anahtar, M. N., Byrne, E. H., Doherty, K. E., Bowman, B. A., Yamamoto, H. S., Soumillon, M., Padavattan, N., Ismail, N., Moodley, A., Sabatini, M. E., et al. (2015) · Immunity · doi:10.1016/j.immuni.2015.04.019 · Cited by 801 · see citing works
Donders, G. G. G., Vereecken, A. J., Bosmans, E., Dekeersmaecker, A., Salembier, G. M., & Spitz, B. (2002) · BJOG An International Journal of Obstetrics & Gynaecology · doi:10.1111/j.1471-0528.2002.00432.x · Cited by 536 · see citing works
The global epidemiology of bacterial vaginosis: a systematic review
Kenyon, C. R., Colebunders, R., & Crucitti, T. (2013) · American Journal of Obstetrics and Gynecology · doi:10.1016/j.ajog.2013.05.006 · Cited by 465 · see citing works
The role of lactobacilli and probiotics in maintaining vaginal health
Borges, S., Silva, J., & Teixeira, P. (2014) · Archives of Gynecology and Obstetrics · doi:10.1007/s00404-013-3064-9 · Cited by 397 · see citing works
Aldunate, M., Srbinovski, D., Hearps, A. C., Latham, C. F., Ramsland, P. A., Gugasyan, R., Cone, R. A., & Tachedjian, G. (2015) · Frontiers in Physiology · doi:10.3389/fphys.2015.00164 · Cited by 387 · see citing works
Elovitz, M. A., Gajer, P., Riis, V. M., Brown, A. G., Humphrys, M. S., Holm, J. B., & Ravel, J. (2019) · Nature Communications · doi:10.1038/s41467-019-09285-9 · Cited by 368 · see citing works
Macklaim, J. M., Fernandes, A. D., Bella, J. M. D., Hammond, J., Reid, G., & Gloor, G. B. (2013) · Microbiome · doi:10.1186/2049-2618-1-12 · Cited by 288 · see citing works
Aerobic vaginitis: no longer a stranger
Donders, G. G. G., Bellen, G., Grincevičienė, Š., Ruban, K., & Vieira‐Baptista, P. (2017) · Research in Microbiology · doi:10.1016/j.resmic.2017.04.004 · Cited by 243 · see citing works
Cervicovaginal microbiota, women's health, and reproductive outcomes
Kroon, S. J., Ravel, J., & Huston, W. M. (2018) · Fertility and Sterility · doi:10.1016/j.fertnstert.2018.06.036 · Cited by 235 · see citing works
Interplay among Vaginal Microbiome, Immune Response and Sexually Transmitted Viral Infections
Torcia, M. G. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20020266 · Cited by 198 · see citing works
Aerobic vaginitis in pregnancy
Donders, G. G. G., Bellen, G., & Rezeberga, D. (2011) · BJOG An International Journal of Obstetrics & Gynaecology · doi:10.1111/j.1471-0528.2011.03020.x · Cited by 192 · see citing works
The global health impact of vaginal dysbiosis
van de Wijgert, J. H. H. M., & Jespers, V. (2017) · Research in Microbiology · doi:10.1016/j.resmic.2017.02.003 · Cited by 189 · see citing works
Species-level classification of the vaginal microbiome
Fettweis, J. M., Serrano, M. G., Sheth, N. U., Mayer, C. M., Glascock, A. L., Brooks, J. P., Jefferson, K. K., & Buck, G. A. (2012) · BMC Genomics · doi:10.1186/1471-2164-13-s8-s17 · Cited by 187 · see citing works
Sugahara, H., Odamaki, T., Fukuda, S., Kato, T., Xiao, J.-Z., Abe, F., Kikuchi, J., & Ohno, H. (2015) · Scientific Reports · doi:10.1038/srep13548 · Cited by 169 · see citing works
Jespers, V., Menten, J., Smet, H., Poradosu, S., Abdellati, S., Verhelst, R., Hardy, L., Buvé, A., & Crucitti, T. (2012) · BMC Microbiology · doi:10.1186/1471-2180-12-83 · Cited by 158 · see citing works
Vaginal Microbiomes Associated With Aerobic Vaginitis and Bacterial Vaginosis
Kaambo, E., Africa, C. W. J., Chambuso, R., & Passmore, J.-A. S. (2018) · Frontiers in Public Health · doi:10.3389/fpubh.2018.00078 · Cited by 158 · see citing works
Ghartey, J. P., Smith, B. C., Chen, Z., Buckley, N. J., Lo, Y., Ratner, A. J., Herold, B. C., & Burk, R. D. (2014) · PLoS ONE · doi:10.1371/journal.pone.0096659 · Cited by 121 · see citing works
Bacterial vaginosis is conducive to the persistence of HPV infection
Guo, Y.-L., You, K., Qiao, J., Zhao, Y.-M., & Geng, L. (2012) · International Journal of STD & AIDS · doi:10.1258/ijsa.2012.011342 · Cited by 116 · see citing works
Freitas, A. C., & Hill, J. E. (2017) · Anaerobe · doi:10.1016/j.anaerobe.2017.05.012 · Cited by 108 · see citing works
Subclinical alteration of the cervical–vaginal microbiome in women with idiopathic infertility
Campisciano, G., Florian, F., D’Eustacchio, A., Stanković, D., Ricci, G., Seta, F. D., & Comar, M. (2017) · Journal of Cellular Physiology · doi:10.1002/jcp.25806 · Cited by 100 · see citing works
Self-citationDonnarumma, G., Molinaro, A., Cimini, D., De Castro, C., Valli, V., De Gregorio, V., De Rosa, M., & Schiraldi, C. (2014) · BMC Microbiology · doi:10.1186/1471-2180-14-137 · Cited by 76 · see citing works
Aerobic vaginitis in late pregnancy and outcomes of pregnancy
Han, C., Li, H., Han, L., Wang, C., Yan, Y., Qi, W., Fan, A., Wang, Y., & Xue, F. (2018) · European Journal of Clinical Microbiology & Infectious Diseases · doi:10.1007/s10096-018-3416-2 · Cited by 71 · see citing works
Biagi, E., Vitali, B., Pugliese, C., Candela, M., Donders, G. G. G., & Brigidi, P. (2008) · European Journal of Clinical Microbiology & Infectious Diseases · doi:10.1007/s10096-008-0617-0 · Cited by 70 · see citing works
Campisciano, G., Zanotta, N., Licastro, D., Seta, F. D., & Comar, M. (2018) · Scientific Reports · doi:10.1038/s41598-018-20649-x · Cited by 66 · see citing works
Self-citationCervical Microbiota in Women with Preterm Prelabor Rupture of Membranes
Kacerovský, M., Vrbacký, F., Kutová, R., Plíšková, L., Andrýs, C., Musilova, I. K., Menon, R., Lamont, R., & Nekvindová, J. (2015) · PLoS ONE · doi:10.1371/journal.pone.0126884 · Cited by 65 · see citing works
Bao, K., Papadimitropoulos, A., Akgũl, B., Belibasakis, G. N., & Bostancı, N. (2015) · Virulence · doi:10.4161/21505594.2014.978721 · Cited by 62 · see citing works
He, L., Yang, X., Liu, C., et al. (2018) · PubMed · Cited by 59 · see citing works
Desquamative inflammatory vaginitis
Reichman, O., & Sobel, J. D. (2014) · Best Practice & Research Clinical Obstetrics & Gynaecology · doi:10.1016/j.bpobgyn.2014.07.003 · Cited by 57 · see citing works
The Vaginal Flora of Healthy Women Is Not Always Dominated byLactobacillusSpecies
Forney, L. J., Foster, J. A., & Ledger, W. J. (2006) · The Journal of Infectious Diseases · doi:10.1086/508497 · Cited by 52 · see citing works
Parolin, C., Frisco, G., Foschi, C., Giordani, B., Salvo, M., Vitali, B., Marangoni, A., & Calonghi, N. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02630 · Cited by 52 · see citing works
Kasprzykowska, U., Joanna, E., Marek, E., et al. (2013) · Archives of Gynecology and Obstetrics · doi:10.1007/s00404-013-3102-7 · Cited by 51 · see citing works
Prevotella bivia can invade human cervix epithelial (HeLa) cells
Strömbeck, L., Sandros, J., Holst, E., Madianos, P., Nannmark, U., Papapanou, P. N., & Mattsby‐Baltzer, I. (2007) · Apmis · doi:10.1111/j.1600-0463.2007.apm_512.x · Cited by 24 · see citing works
High Concentration but Low Activity of Hepatocyte Growth Factor in Periodontitis
Lönn, J., Johansson, C. S., Nakka, S. S., Palm, E. C., Bengtsson, T., Nayeri, F., & Ravald, N. (2013) · Journal of Periodontology · doi:10.1902/jop.2013.130003 · Cited by 23 · see citing works
Zanotta, N., Monasta, L., Skerk, K., Luppi, S., Martinelli, M., Ricci, G., & Comar, M. (2018) · American Journal of Reproductive Immunology · doi:10.1111/aji.13064 · Cited by 17 · see citing works
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Viral Infections, the Microbiome, and Probiotics
Harper, A., Vijayakumar, V., Ouwehand, A. C., ter Haar, J., Obis, D., Espadaler, J., Binda, S., Desiraju, S., & Day, R. (2021) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2020.596166 · Cited by 177 · see citing works
- Authors
- De Seta, F., Campisciano, G., Zanotta, N., Ricci, G., & Comar, M.
- Affiliations
- IRCCS Materno Infantile Burlo Garofolo (IT)
- University of Trieste (IT)
- Venue
- Frontiers in Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2019-10-30
- Volume
- 10
- Pages
- 2451-2451
- ISSN
- 1664-302X (ISSN-L)
- PMC
- PMC6831638
- OpenAlex
- W2981949466
- Cited by
- 134
- References
- 38
- Citations by year
- 2026: 19 · 2025: 22 · 2024: 21 · 2023: 28 · 2022: 14 · 2021: 16 · 2020: 10 · 2019: 2 · 2018: 1
- FWCI
- 7.71
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Microbiology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- ArTS Archivio della ricerca di Trieste (University of Trieste https://www.units.it/) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Ministero della Salute
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- bacterial vaginosis, aerobic vaginitis, vaginal microbiota, community state types, Lactobacillus, Bifidobacterium, vaginal dysbiosis, inflammation, asymptomatic women
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