Lactobacillus crispatus L1: high cell density cultivation and exopolysaccharide structure characterization to highlight potentially beneficial effects against vaginal pathogens

Donnarumma, Giovanna; Molinaro, Antonio; Cimini, Donatella; De Castro, Cristina; Valli, Vivien; De Gregorio, Vincenza; De Rosa, Mario; Schiraldi, Chiara

doi:10.1186/1471-2180-14-137

Cited by 76Probiotics and Fermented FoodsReproductive tract infections researchPolysaccharides Composition and Applications

Lactobacillus crispatus L1: high cell density cultivation and exopolysaccharide structure characterization to highlight potentially beneficial effects against vaginal pathogens

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Donnarumma, Giovanna, Molinaro, Antonio, Cimini, Donatella, De Castro, Cristina, Valli, Vivien, De Gregorio, Vincenza, De Rosa, Mario, Schiraldi, Chiara·2014·BMC Microbiology

DOI 10.1186/1471-2180-14-137PMC PMC4054921

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Abstract

BACKGROUND: Vaginal lactic acid bacteria defend the host against pathogens through a combination of competitive exclusion, competition for nutrients, production of antimicrobial substances and through the activation of the immune system. A new human isolate named Lactobacillus crispatus L1 was characterized in this work, and a preliminary evaluation of its probiotic potential is described together with a process to obtain a high productivity of viable biomass. RESULTS: In a simulated digestion process 1.8⋅10(10) cells∙ml(-1) survived the gastric environment with 80% viability, without being affected by small intestine juices. Experiments on six different C sources were performed to analyze growth and organic acids production and, glucose, provided the best performances. A microfiltration strategy was exploited to improve the cellular yield in 2 L-fermentation processes, reaching 27 g · l(-1) of dry biomass. Moreover, L. crispatus L1 demonstrated a greater stability to high concentrations of lactic acid, compared to other lactobacilli. The specific L. crispatus L1 exopolysaccharide was purified from the fermentation broth and characterized by NMR showing structural features and similarity to exopolysaccharides produced by pathogenic strains. Live L. crispatus L1 cells strongly reduced adhesion of a yeast pathogenic strain, Candida albicans in particular, in adherence assays. Interestingly a higher expression of the human defensin HBD-2 was also observed in vaginal cells treated with the purified exopolysaccharide, indicating a possible correlation with C. albicans growth inhibition. CONCLUSIONS: The paper describes the evaluation of L. crispatus L1 as potential vaginal probiotic and the fermentation processes to obtain high concentrations of viable cells.

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EPS is lyophilized to remove moisture and allow long storage.
“Finally, the EPS is lyophilized [27,33,35,43,99,101] to remove moisture and allow long storage.”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [101]
The Methods section (“EPSs purification and quantification”; page unavailable) says the concentrated EPS solution was lyophilized and the resulting powder used for characterization. This supports lyophilization and is consistent with moisture removal, but the paper does not say it was done to allow long storage. (LLM, conf=0.86)
Experimental — accuracy not yet measured
Membrane filtration techniques such as microfiltration, ultrafiltration and/or diafiltration have been used as a preceding step to EPS purification.
“As a preceding step to EPS purification, membrane filtration techniques such as microfiltration, ultrafiltration and/or diafiltration have also been used [50,58,70,101].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [101]

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In Vitro Assessment of Lactobacillus crispatus UBLCp01, Lactobacillus gasseri UBLG36, and Lactobacillus johnsonii UBLJ01 as a Potential Vaginal Probiotic Candidate
Ahire, J. J., Sahoo, S., Kashikar, M. S., Heerekar, A., Lakshmi, S. G., & Madempudi, R. S. (2021) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-021-09838-9 · Cited by 39 · see citing works
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Production and characterization of a novel exopolysaccharide from Lactobacillus crispatus L1 in high cell density cultivation
SCHIRALDI, C., Valli, V., Cimini, D., Donnarumma, G., Molinaro, A., & ROSA, M. D. (2009) · Archivio Istituzionale della Ricerca - Università degli Studi della Campania "Luigi Vanvitelli"
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Authors: Donnarumma, G., Molinaro, A., Cimini, D., De Castro, C., Valli, V., De Gregorio, V., De Rosa, M., & Schiraldi, C.
Affiliations: University of Campania "Luigi Vanvitelli" (IT)
University of Naples Federico II (IT)
Corresponding author: Schiraldi, Chiara
ORCID: Donnarumma, Giovanna
Molinaro, Antonio
Cimini, Donatella
Schiraldi, Chiara
Venue: BMC Microbiology
Publisher: Springer Science and Business Media LLC
Published: 2014-05-30
Volume: 14
Issue: 1
Pages: 137-137
ISSN: 1471-2180 (ISSN-L)
DOI: 10.1186/1471-2180-14-137
PMC: PMC4054921
OpenAlex: W1975425344
Cited by: 76
References: 47
Citations by year: 2026: 6 · 2025: 1 · 2024: 6 · 2023: 8 · 2022: 8 · 2021: 8 · 2020: 10 · 2019: 9 · 2018: 4 · 2017: 7 · 2016: 2 · 2015: 6 · 2014: 1
FWCI: 7.63
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
Locations: BMC Microbiology (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
Archivio Istituzionale della Ricerca - Università degli Studi della Campania "Luigi Vanvitelli" (submittedVersion)
Funders: Ministero dell’Istruzione, dell’Università e della Ricerca
Topics: Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
Keywords: vaginal probiotics, high cell density culture, microfiltration, in vitro digestion, lactic acid tolerance, Candida albicans adhesion, vaginal pathogens, organic acid production
MeSH terms: Antibiosis, Candida albicans, Carboxylic Acids, Cell Adhesion, Cell Count, Female, Glucose, Humans, Lactobacillus, Magnetic Resonance Spectroscopy, Pancreatic Juice, Polysaccharides, Bacterial, Vagina, Probiotics, Microbial Viability, Microbial Interactions

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Lactobacillus crispatus L1: high cell density cultivation and exopolysaccharide structure characterization to highlight potentially beneficial effects against vaginal pathogens

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