Complete Genome Sequence of the Newly Developed Lactobacillus acidophilus Strain With Improved Thermal Adaptability
Open accessAbstract
Lactobacillus acidophilus (L. acidophilus) is a representative probiotic and is widely used in many industrial products for its beneficial effects on human and animal health. This bacterium is exposed to harsh environments such as high temperatures for manufacturing industrial products, but cell yield under high temperatures is relatively low. To resolve this issue, we developed a new L. acidophilus strain with improved heat resistance while retaining the existing beneficial properties through the adaptive laboratory evolution (ALE) method. The newly developed strain, L. acidophilus EG008, has improved the existing limit of thermal resistance from 65°C to 75°C. Furthermore, we performed whole-genome sequencing and comparative genome analysis of wild-type and EG008 strains to unravel the molecular mechanism of improved heat resistance. Interestingly, only two single-nucleotide polymorphisms (SNPs) were different compared to the L. acidophilus wild-type. We identified that one of these SNPs is a non-synonymous SNP capable of altering the structure of MurD protein through the 435th amino acid change from serine to threonine. We believe that these results will directly contribute to any industrial field where L. acidophilus is applied. In addition, these results make a step forward in understanding the molecular mechanisms of lactic acid bacteria evolution under extreme conditions.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
LAB with high resistance to harsh conditions such as acidic pH and bile salts colonize various environments and offer numerous health benefits due to their probiotic properties.
“With high resistance to harsh conditions such as acidic pH and bile salts, LAB not only colonize various environments, but also offer numerous health benefits due to their probiotic properties4.”
From Probiotic potential of Lactobacillus acidophilus TMPC 41127 isolated from traditional cheese in inhibiting Salmonella Typhi biofilms and virulence genes (2026) · cites this paper as [4]
The Introduction (page not available in our copy) says LAB resist acids and bile salts and can inhibit pathogens; it also describes several health benefits of L. acidophilus, a LAB species. It does not say that this resistance enables LAB to colonize various environments. (LLM, conf=0.85)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms
Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018) · Molecular Biology and Evolution · doi:10.1093/molbev/msy096 · Cited by 37,683 · see citing works
Clustal W and Clustal X version 2.0
Larkin, M., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A., López, R., et al. (2007) · Bioinformatics · doi:10.1093/bioinformatics/btm404 · Cited by 28,221 · see citing works
The RAST Server: Rapid Annotations using Subsystems Technology
Aziz, R. K., Bartels, D., Best, A. A., DeJongh, M., Disz, T. L., Edwards, R. A., Formsma, K., Gerdes, S., Glass, E. M., Kubal, M., et al. (2008) · BMC Genomics · doi:10.1186/1471-2164-9-75 · Cited by 11,777 · see citing works
Shifting the genomic gold standard for the prokaryotic species definition
Richter, M., & Rossello-Mora, R. A. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0906412106 · Cited by 6,343 · see citing works
RNAmmer: consistent and rapid annotation of ribosomal RNA genes
Lagesen, K., Hallin, P., Rødland, E., Stærfeldt, H., Rognes, T., & Ussery, D. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm160 · Cited by 5,965 · see citing works
Versatile and open software for comparing large genomes
Kurtz, S., Phillippy, A., Delcher, A. L., Smoot, M., Shumway, M., Antonescu, C., & Salzberg, S. L. (2004) · Genome Biology · doi:10.1186/gb-2004-5-2-r12 · Cited by 5,254 · see citing works
NCBI BLAST: a better web interface
Johnson, M., Zaretskaya, I., Raytselis, Y., Merezhuk, Y., McGinnis, S., & Madden, T. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkn201 · Cited by 4,207 · see citing works
PHASTER: a better, faster version of the PHAST phage search tool
Arndt, D., Grant, J. R., Marcu, A., Sajed, T., Pon, A., Liang, Y., & Wishart, D. S. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw387 · Cited by 3,496 · see citing works
Zheng, J., Wittouck, S., Salvetti, E., Franz, C. M. A. P., Harris, H. M. B., Mattarelli, P., O’Toole, P. W., Pot, B., Vandamme, P., Walter, J., et al. (2020) · International Journal of Systematic and Evolutionary Microbiology · doi:10.1099/ijsem.0.004107 · Cited by 3,313 · see citing works
Artemis: sequence visualization and annotation
Rutherford, K. M., Parkhill, J., Crook, J., Horsnell, T., Rice, P., Rajandream, M., & Barrell, B. (2000) · Bioinform. · doi:10.1093/bioinformatics/16.10.944 · Cited by 3,295 · see citing works
CARD 2017: expansion and model-centric curation of the comprehensive antibiotic resistance database
Jia, B., Raphenya, A. R., Alcock, B. P., Waglechner, N., Guo, P., Tsang, K. K., Lago, B. A., Dave, B. M., Pereira, S. K., Sharma, A. N., et al. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw1004 · Cited by 2,671 · see citing works
IslandViewer 4: expanded prediction of genomic islands for larger-scale datasets
Bertelli, C., Laird, M. R., Williams, K. P., Lau, B. Y., Hoad, G. R., Winsor, G. L., & Brinkman, F. S. L. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx343 · Cited by 1,535 · see citing works
ARG-ANNOT, a new bioinformatic tool to discover antibiotic resistance genes in bacterial genomes
Gupta, S. K., Padmanabhan, B. R., Diene, S. M., Lopez-Rojas, R., Kempf, M., Landraud, L., & Rolain, J.-M. (2014) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01310-13 · Cited by 1,476 · see citing works
Joensen, K., Scheutz, F., Lund, O., Hasman, H., Kaas, R. S., Nielsen, E. M., & Aarestrup, F. M. (2014) · Journal of Clinical Microbiology · doi:10.1128/jcm.03617-13 · Cited by 1,394 · see citing works
Comparative genomics of the lactic acid bacteria.
Makarova, K., Slesarev, A., Wolf, Y., Sorokin, A., Mirkin, B., Koonin, E., Pavlov, A., Pavlova, N., Karamychev, V., Polouchine, N., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0607117103 · Cited by 1,391 · see citing works
Bacteriocin-based strategies for food biopreservation
Gálvez, A., Abriouel, H., López, R. L., & Benomar, N. (2007) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2007.06.001 · Cited by 1,224 · see citing works
Circlator: automated circularization of genome assemblies using long sequencing reads
Hunt, M., Silva, N. H. D., Otto, T. D., Parkhill, J., Keane, J. A., & Harris, S. R. (2015) · Genome biology · doi:10.1186/s13059-015-0849-0 · Cited by 1,166 · see citing works
BAGEL4: a user-friendly web server to thoroughly mine RiPPs and bacteriocins
van Heel, A. J., de Jong, A., Song, C., Viel, J. H., Kok, J., & Kuipers, O. P. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky383 · Cited by 1,134 · see citing works
Wang, Y., Coleman‐Derr, D., Chen, G., & Gu, Y. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv487 · Cited by 888 · see citing works
Xu, L., Dong, Z., Lü, F., Luo, Y., Wei, Z., Guo, H., Zhang, G., Gu, Y., Coleman‐Derr, D., Xia, Q., et al. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz333 · Cited by 850 · see citing works
Adaptive laboratory evolution—Principles and applications for biotechnology
Dragosits, M., & Mattanovich, D. (2013) · Microbial Cell Factories · doi:10.1186/1475-2859-12-64 · Cited by 827 · see citing works
Bacteriocin Production: a Probiotic Trait?
Dobson, A., Cotter, P. D., Ross, R. P., & Hill, C. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.05576-11 · Cited by 690 · see citing works
Heat stress effects on livestock: molecular, cellular and metabolic aspects, a review
Slimen, I. B., Najar, T., Ghram, A., & Abderrabba, M. (2015) · Journal of Animal Physiology and Animal Nutrition · doi:10.1111/jpn.12379 · Cited by 636 · see citing works
Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM
Altermann, E., Russell, W. M., Azcárate-Peril, M. A., Barrangou, R., Buck, B. L., McAuliffe, O. E., Souther, N. E., Dobson, A., Duong, T., Callanan, M. J., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0409188102 · Cited by 594 · see citing works
PGAP: pan-genomes analysis pipeline
Zhao, Y., Wu, J., Yang, J., Sun, S., Xiao, J., & Yu, J. M. (2011) · Bioinformatics · doi:10.1093/bioinformatics/btr655 · Cited by 518 · see citing works
Chou, L., & Weimer, B. C. (1999) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(99)75204-5 · Cited by 343 · see citing works
Adaptive laboratory evolution — harnessing the power of biology for metabolic engineering
Portnoy, V. A., Bezdan, D., & Zengler, K. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.03.007 · Cited by 309 · see citing works
Sengupta, R., Altermann, E., Anderson, R. C., McNabb, W. C., Moughan, P. J., & Roy, N. C. (2013) · Mediators of Inflammation · doi:10.1155/2013/237921 · Cited by 287 · see citing works
Gupta, R., Lavollay, M., Mainardi, J.-L., Arthur, M., Bishai, W. R., & Lamichhane, G. (2010) · Nature Medicine · doi:10.1038/nm.2120 · Cited by 281 · see citing works
Microbial growth and substrate utilization kinetics
Okpokwasili, G. C., & Nweke, C. O. (2006) · TSpace (University of Toronto) · doi:10.5897/ajb2006.000-5041 · Cited by 256 · see citing works
MOLECULAR MECHANISMS OF BACTERIOCIN EVOLUTION
Riley, M. A. (1998) · Annual Review of Genetics · doi:10.1146/annurev.genet.32.1.255 · Cited by 249 · see citing works
Domestication of Industrial Microbes
Steensels, J., Gallone, B., Voordeckers, K., & Verstrepen, K. J. (2019) · Current Biology · doi:10.1016/j.cub.2019.04.025 · Cited by 214 · see citing works
Salmonella in Commercially Manufactured Feeds
JONES, F. T., & Richardson, K. E. (2004) · Poultry Science · doi:10.1093/ps/83.3.384 · Cited by 195 · see citing works
Interaction of bacteria and host cell walls: its relation to mechanisms of induced resistance
Sequeira, L., Gaard, G., & de Zoeten, G. A. (1977) · Physiological Plant Pathology · doi:10.1016/0048-4059(77)90006-6 · Cited by 175 · see citing works
Hugonnet, J., Mengin‐Lecreulx, D., Silva, A. M., den Blaauwen, T., Carbonnelle, É., Veckerlé, C., Brun, Y. V., VanNieuwenhze, M. S., Bouchier, C., Tu, K., et al. (2016) · eLife · doi:10.7554/elife.19469 · Cited by 161 · see citing works
ppGpp: stringent response and survival.
Jain, V. P., Kumar, M., & Chatterji, D. (2006) · PubMed · Cited by 158 · see citing works
Evolution of Escherichia coli for Growth at High Temperatures
Rudolph, B., Gebendorfer, K. M., Büchner, J., & Winter, J. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.103374 · Cited by 156 · see citing works
Park, J., Choi, J. W., Jhun, J., Kwon, J. Y., Lee, B., Yang, C. W., Park, S., & Cho, M. (2018) · Journal of Medicinal Food · doi:10.1089/jmf.2017.3990 · Cited by 154 · see citing works
Vaudagna, S. R., Sánchez, G. C., Neira, M. S., Insani, E. M., Picallo, A., Gallinger, M. M., & Lasta, J. A. (2002) · International Journal of Food Science & Technology · doi:10.1046/j.1365-2621.2002.00581.x · Cited by 148 · see citing works
Crystal structure of UDP‐N‐acetylmuramoyl‐L‐alanine:D‐glutamate ligase from Escherichia coli
Bertrand, J. A., Auger, G., Fanchon, É., Martin, L., Blanot, D., van HEIJENOORT, J., & Dideberg, O. (1997) · The EMBO Journal · doi:10.1093/emboj/16.12.3416 · Cited by 143 · see citing works
Bacteriocin production: A relatively unharnessed probiotic trait?
Hegarty, J. W., Guinane, C. M., Ross, R. P., Hill, C., & Cotter, P. D. (2016) · F1000Research · doi:10.12688/f1000research.9615.1 · Cited by 141 · see citing works
Chen, M., Tang, H.-Y., & Chiang, M. (2017) · Food Microbiology · doi:10.1016/j.fm.2017.03.020 · Cited by 140 · see citing works
Zhuo, Q., Yu, B., Zhou, J., Zhang, J., Zhang, R., Xie, J., Wang, Q., & Zhao, S. (2019) · Scientific Reports · doi:10.1038/s41598-019-56661-y · Cited by 138 · see citing works
Klein, A., Friedrich, U., Vogelsang, H., & Jahreis, G. (2007) · European Journal of Clinical Nutrition · doi:10.1038/sj.ejcn.1602761 · Cited by 136 · see citing works
Johann, P., Courtin, P., Meouche, I. E., Lemée, L., Chapot‐Chartier, M., & Pons, J.-L. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.259150 · Cited by 133 · see citing works
Assessment of Stress Response of the Probiotic Lactobacillus acidophilus
Kim, W. S., Perl, L., Park, J. H., Tandianus, J. E., & Dunn, N. W. (2001) · Current Microbiology · doi:10.1007/s002840010314 · Cited by 127 · see citing works
Identification and Characterization of Lactic Acid Bacteria in a Commercial Probiotic Culture
Menconi, A., Kallapura, G., Latorre, J. D., Morgan, M. J., Pumford, N. R., Hargis, B. M., & Téllez‐Isaías, G. (2014) · Bioscience of Microbiota Food and Health · doi:10.12938/bmfh.33.25 · Cited by 121 · see citing works
Xu, M., Gagné-Bourque, F., Dumont, M., & Jabaji, S. (2015) · Journal of Food Engineering · doi:10.1016/j.jfoodeng.2015.07.021 · Cited by 120 · see citing works
Perán, L., Camuesco, D., Comalada, M., Bailón, E., Henriksson, A., Xaus, J., Zarzuelo, A., & Gálvez, J. (2007) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2007.03302.x · Cited by 118 · see citing works
Leenay, R. T., Vento, J. M., Shah, M., Martino, M. E., Leulier, F., & Beisel, C. L. (2018) · Biotechnology Journal · doi:10.1002/biot.201700583 · Cited by 115 · see citing works
Haldar, S., Ghosh, T. K., Toshiwati, & Bedford, M. R. (2011) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2011.03.007 · Cited by 113 · see citing works
Foysal, M. J., Fotedar, R. K., Siddik, M. A. B., & Tay, C. Y. (2020) · Scientific Reports · doi:10.1038/s41598-020-62655-y · Cited by 107 · see citing works
Riehle, M. M., Bennett, A. F., Lenski, R. E., & Long, A. D. (2003) · Physiological Genomics · doi:10.1152/physiolgenomics.00034.2002 · Cited by 103 · see citing works
The effect of steam-conditioning rate on the pelleting process
Skoch, E. R., Behnke, K. C., DEYOE, C. W., & Binder, S. F. (1981) · Animal Feed Science and Technology · doi:10.1016/0377-8401(81)90033-x · Cited by 96 · see citing works
Bernal-Cabas, M., Ayala, J. A., & Raivio, T. L. (2014) · Journal of Bacteriology · doi:10.1128/jb.02449-14 · Cited by 92 · see citing works
Genomic analyses of thermotolerant microorganisms used for high-temperature fermentations
Matsushita, K., Azuma, Y., Kosaka, T., Yakushi, T., Hoshida, H., Akada, R., & Yamada, M. C. (2015) · Bioscience Biotechnology and Biochemistry · doi:10.1080/09168451.2015.1104235 · Cited by 81 · see citing works
Effects of Lactobacillus rhamnosus and Lactobacillus acidophilus on bacterial vaginal pathogens
Bertuccini, L., Russo, R., Iosi, F., & Superti, F. (2017) · International Journal of Immunopathology and Pharmacology · doi:10.1177/0394632017697987 · Cited by 80 · see citing works
Matijašić, B. B., Obermajer, T., Lipoglavšek, L., Sernel, T., Locatelli, I., Kos, M., Šmid, A., & Rogelj, I. (2016) · Journal of Dairy Science · doi:10.3168/jds.2015-10743 · Cited by 79 · see citing works
Betaine Transport Imparts Osmotolerance on a Strain of Lactobacillus acidophilus
Hutkins, R. W., Ellefson, W. L., & Kashket, E. R. (1987) · Applied and Environmental Microbiology · doi:10.1128/aem.53.10.2275-2281.1987 · Cited by 72 · see citing works
Heat resistance of Lactobacillus spp. isolated from Cheddar cheese
JORDAN, K. N., & Cogan, T. M. (1999) · Letters in Applied Microbiology · doi:10.1046/j.1365-2672.1999.00607.x · Cited by 71 · see citing works
Resistance of functional Lactobacillus plantarum strains against food stress conditions
Ferrando, V., Quiberoni, A. D. L., Reinhemer, J., & Suárez, V. (2014) · Food Microbiology · doi:10.1016/j.fm.2014.12.005 · Cited by 68 · see citing works
Stress-induced adaptive morphogenesis in bacteria
Ultee, E., Ramijan, K., Dame, R. T., Briegel, A., & Claessen, D. (2019) · Advances in microbial physiology/Advances in Microbial Physiology · doi:10.1016/bs.ampbs.2019.02.001 · Cited by 65 · see citing works
Mengin‐Lecreulx, D., Falla, T., Blanot, D., van HEIJENOORT, J., Adams, D. J., & Chopra, I. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.19.5909-5914.1999 · Cited by 64 · see citing works
Basta, L. A. B., Ghosh, A., Pan, Y. H., Jakoncic, J., Lloyd, E. P., Townsend, C. A., Lamichhane, G., & Bianchet, M. A. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.660753 · Cited by 55 · see citing works
Nguyen, A. T. V., Nguyen, D. V., Tran, M. T., Nguyen, A. H., Nguyen, A. H., & Nghĩa, P. T. (2015) · Letters in Applied Microbiology · doi:10.1111/lam.12411 · Cited by 54 · see citing works
Chahuki, F. F., Aminzadeh, S., Jafarian, V., Tabandeh, F., & Khodabandeh, M. (2018) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2018.10.112 · Cited by 54 · see citing works
Schillinger, U., Yousif, N. M. K., Sesar, L., & Franz, C. M. A. P. (2003) · Current Microbiology · doi:10.1007/s00284-003-4067-8 · Cited by 51 · see citing works
Transcriptome Analysis of Adaptive Heat Shock Response of Streptococcus thermophilus
Li, J., Bi, Y.-T., Dong, C., Yang, J., & Liang, W. (2011) · PLoS ONE · doi:10.1371/journal.pone.0025777 · Cited by 51 · see citing works
Palomino, M. M., Waehner, P. M., Fina-Martin, J., Ojeda, P., Malone, L. M., Sánchez-Rivas, C., Prado-Acosta, M., Allievi, M., & Ruzal, S. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-7698-y · Cited by 48 · see citing works
Mohankumar, A., & Murugalatha, N. (2011) · International Journal of Biology · doi:10.5539/ijb.v3n3p128 · Cited by 46 · see citing works
Khaleghi, M., Kermanshahi, R. K., Yaghoobi, M. M., Zarkesh‐Esfahani, S. H., & Baghizadeh, A. (2010) · PubMed · Cited by 45 · see citing works
Kulkarni, S., Haq, S. F., Samant, S., & Sukumaran, S. (2017) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-017-9321-7 · Cited by 42 · see citing works
Lan, R., Koo, J., & Kim, I. H. (2016) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.7866 · Cited by 37 · see citing works
S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions
Grosu‐Tudor, S., Brown, L., Hebert, E. M., Brezeanu, A., Brînzan, A., Fadda, S., Mozzi, F., & Zamfir, M. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-7355-5 · Cited by 36 · see citing works
Skin Moisturizing and Antiphotodamage Effects of Tyndallized Lactobacillus acidophilus IDCC 3302
Im, A., Lee, B., Kang, D., & Chae, S. (2018) · Journal of Medicinal Food · doi:10.1089/jmf.2017.4100 · Cited by 33 · see citing works
Boney, J. W., Jaczynski, J., Weidhaas, J., Bergeron, A. N., & Moritz, J. S. (2018) · The Journal of Applied Poultry Research · doi:10.3382/japr/pfy052 · Cited by 27 · see citing works
Min, B., Yoo, D., Lee, Y. H., Seo, M., & Kim, H. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00828 · Cited by 21 · see citing works
Self-citationPossibilities of the use of recombinant-DNA techniques with rumen micro-organisms
Orpin, C. G., Hazlewood, G. P., & Mann, S. P. (1988) · Animal Feed Science and Technology · doi:10.1016/0377-8401(88)90097-1 · Cited by 10 · see citing works
Meena, S., Mehla, J., Kumar, R., & Sood, S. K. (2016) · Current Microbiology · doi:10.1007/s00284-016-1090-0 · Cited by 6 · see citing works
Janée, G. (2017) · Encyclopedia of Database Systems · doi:10.1007/978-1-4899-7993-3_879-3 · Cited by 1 · see citing works
Flagged first, then by citations
1 of the 81 references couldn’t be matched to a known paper.
Research works printed but not linked: 1
- Dash S. K. (2004). Review of scientific evidence for efficacy of Lactobacillus acidophilus DDS-1 as a probiotic strain. Agro Food Ind. Hi Tech 15 23– 26.
Cited by
Sorted by citations
Research progress on improving the freeze-drying resistance of probiotics: A review
Ge, S., Han, J., Sun, Q., Zhou, Q., Ye, Z., Li, P., & Gu, Q. (2024) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2024.104425 · Cited by 114 · see citing works
Mörschbächer, A. P., & Granada, C. E. (2022) · Biochemical Engineering Journal · doi:10.1016/j.bej.2022.108343 · Cited by 58 · see citing works
Probiotic Development Strategy Centered on Stability and Regulatory Considerations
Kim, H., Haque, M. A., Razzak, M. A., Jang, M. J., Song, S., & Ku, S. (2026) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.70320 · Cited by 43 · see citing works
Self-citationLactobacillomics as a new notion in lactic acid bacteria research through omics integration
Ilıkkan, Ö. K. (2025) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-025-04285-y · Cited by 25 · see citing works
Jeon, S., Kim, H., Kim, J., Seol, D., Jo, J., Choi, Y., Cho, S., & Kim, H. (2022) · Microbiology Spectrum · doi:10.1128/spectrum.01815-21 · Cited by 21 · see citing works
Self-citationThermotolerant Probiotic—The Potential of Improving the Survivability of Beneficial Bacteria
Zielińska, D., Krawczyk, M., & Neffe‐Skocińska, K. (2025) · Fermentation · doi:10.3390/fermentation11060313 · Cited by 13 · see citing works
Huang, Q., Zhang, H., Zhang, L., & Xu, B. (2023) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2023.2272770 · Cited by 12 · see citing works
Li, Y., Li, H., Wu, H., Sun, X., Liu, S., Zhang, D., Su, C., & He, Z. (2024) · Food Research International · doi:10.1016/j.foodres.2024.114544 · Cited by 11 · see citing works
Alizadeh Behbahani, B., Ghasemi, P., Vasiee, A., & Mousavi, Z. (2026) · Scientific Reports · doi:10.1038/s41598-026-51296-2 · Cited by 11 · see citing works
Sun, Z., Liu, L., Zhang, H., Wang, Y., Liu, J., Wang, Z., Mu, D., Wu, X., & Li, X. (2024) · Food Bioscience · doi:10.1016/j.fbio.2024.105401 · Cited by 7 · see citing works
Gao, R., Bo, X., Wang, H., Yuan, B., Zhao, X., Li, J., Kwok, L., & Bao, Q. (2025) · LWT · doi:10.1016/j.lwt.2025.118387 · Cited by 7 · see citing works
Hsu, Y.-C., Su, P.-R., Huang, L.-J., Cheng, K., Chen, C., & Hsu, C. (2023) · doi:10.7554/elife.88491.2 · Cited by 7 · see citing works
Zhao, J., Gong, J., Liang, W., & Zhang, S. (2024) · Scientific Reports · doi:10.1038/s41598-024-80947-5 · Cited by 6 · see citing works
Bucka-Kolendo, J., & Sokołowska, B. (2022) · Quality Assurance and Safety of Crops & Foods · doi:10.15586/qas.v14i0.1118 · Cited by 6 · see citing works
Yang, Y., Wang, R., Chen, Z., Yang, Y., Jingjing, E., Gao, A., & Wang, J. (2023) · Drying Technology · doi:10.1080/07373937.2023.2297265 · Cited by 5 · see citing works
Pan, M., O’Flaherty, S., Hibberd, A. A., Gerdes, S., Morovic, W., & Barrangou, R. (2025) · BMC Genomics · doi:10.1186/s12864-024-11177-2 · Cited by 2 · see citing works
Ertürkmen, P. (2025) · Journal of Food Quality · doi:10.1155/jfq/4406517 · Cited by 2 · see citing works
Hsu, Y.-C., Su, P.-R., Huang, L., Cheng, K., Chen, C., & Hsu, C. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.04.17.537164 · Cited by 2 · see citing works
Hsu, Y.-C., Su, P.-R., Huang, L.-J., Cheng, K., Chen, C., & Hsu, C. (2023) · doi:10.7554/elife.88491 · Cited by 2 · see citing works
Screening of molecular elements and improvement of heat resistance in a thermophilic bacterium
Cui, J., Li, C., Cao, G., Wu, Y., Xu, S., Zhang, Y., Bian, X., Tu, Q., & Zheng, W. (2025) · Engineering Microbiology · doi:10.1016/j.engmic.2025.100225 · Cited by 1 · see citing works
Hsu, Y.-C., Su, P.-R., Huang, L.-J., Cheng, K., Chen, C.-H., & Hsu, C. (2023) · doi:10.7554/elife.88491.1
Next-generation probiotics: Advancements in fermented dairy products and their health benefits
Ajmal, A., Yang, J., Ajmal, R., & Mahmud, M. Z. A. (2025) · Traditional Medicine and Modern Medicine · doi:10.1142/s2575900025300048
Wang, Y., Wang, L., Luo, L., Ning, F., Zhao, H., & Li, J. (2025) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.5c07920
gallant, M., Deumić, S., Kovačić, D., & Avdić, M. (2026) · World Congress on Medical Physics and Biomedical Engineering, September 7 - 12, 2009, Munich, Germany · doi:10.1007/978-3-032-06531-5_71
- Authors
- Jeon, S., Kim, H., Choi, Y., Cho, S., Seo, M., & Kim, H.
- Affiliations
- Seoul National University (KR)
- Korea University (KR)
- Corresponding author
- Seo, Minseok
- Kim, Heebal
- Venue
- Frontiers in Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2021-09-24
- Volume
- 12
- Pages
- 697351-697351
- ISSN
- 1664-302X (ISSN-L)
- PMC
- PMC8498822
- OpenAlex
- W3202847747
- Cited by
- 23
- References
- 81
- Citations by year
- 2026: 2 · 2025: 8 · 2024: 4 · 2023: 6 · 2022: 3
- FWCI
- 1.94
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Microbiology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Research Foundation (2021R1A5A8032895)
- Korea University (2021R1A5A8032895)
- National Research Foundation of Korea (2021R1A5A8032895)
- Ministry of Science and ICT, South Korea (2021R1A5A8032895)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Lactobacillus acidophilus, heat resistance, thermal adaptability, adaptive laboratory evolution, probiotics, whole genome sequencing, comparative genomics, single nucleotide polymorphisms
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
