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Cited by 5Heat shock proteins researchAdvanced Electron Microscopy Techniques and ApplicationsProtein Structure and Dynamics

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Cryo-EM structure of the diapause chaperone artemin

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Parvate, Amar D., Powell, Samantha M., Brookreson, Jory T., Moser, Trevor H., Novikova, Irina V., Zhou, Mowei, Evans, James E.·2022·Frontiers in Molecular Biosciences
DOI 10.3389/fmolb.2022.998562PMC PMC9742257

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Abstract

The protein artemin acts as both an RNA and protein chaperone and constitutes over 10% of all protein in Artemia cysts during diapause. However, its mechanistic details remain elusive since no high-resolution structure of artemin exists. Here we report the full-length structure of artemin at 2.04 Å resolution. The cryo-EM map contains density for an intramolecular disulfide bond between Cys22-Cys61 and resolves the entire C-terminus extending into the core of the assembled protein cage but in a different configuration than previously hypothesized with molecular modeling. We also provide data supporting the role of C-terminal helix F towards stabilizing the dimer form that is believed to be important for its chaperoning activity. We were able to destabilize this effect by placing a tag at the C-terminus to fully pack the internal cavity and cause limited steric hindrance.

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Metadata

Authors
Parvate, A. D., Powell, S. M., Brookreson, J. T., Moser, T. H., Novikova, I. V., Zhou, M., & Evans, J. E.
Affiliations
Pacific Northwest National Laboratory (US)
Corresponding author
Evans, James E.
ORCID
Venue
Frontiers in Molecular Biosciences
Publisher
Frontiers Media SA
Published
2022-11-28
Volume
9
Pages
998562-998562
ISSN
2296-889X (ISSN-L)
DOI
10.3389/fmolb.2022.998562
PMC
PMC9742257
OpenAlex
W4310266620
Cited by
5
References
4
Citations by year
2025: 3 · 2024: 2
FWCI
0.41
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Molecular Biosciences (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
Funders
Biological and Environmental Research (74915)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Keywords
artemin, diapause, molecular chaperones, protein structure, intramolecular disulfide bond, C-terminal helix, protein dimerization, cysts, protein cages, steric hindrance

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