Microglia Morphological Categorization in a Rat Model of Neuroinflammation by Hierarchical Cluster and Principal Components Analysis
Open accessAbstract
It is known that microglia morphology and function are closely related, but only few studies have subjectively described different morphological subtypes. To address this issue, morphological parameters of microglial cells were analyzed in a rat model of aseptic neuroinflammation. After the injection of a single dose of the enzyme neuraminidase within the lateral ventricle an acute inflammatory process occurs. Sections from NA-injected animals and sham controls were immunolabeled with the microglial marker IBA1, which highlights ramifications and features of the cell shape. Using images obtained by section scanning, individual microglial cells were sampled from various regions (septofimbrial nucleus, hippocampus and hypothalamus) at different times post-injection (2, 4 and 12 hours). Each cell yielded a set of 15 morphological parameters by means of image analysis software. Five initial parameters (including fractal measures) were statistically different in cells from NA-injected rats (most of them IL-1β positive, i.e. M1-state) compared to those from control animals (none of them IL-1β positive, i.e. surveillant state). However, additional multimodal parameters were revealed more suitable for hierarchical cluster analysis. This method pointed out the classification of microglia population in four clusters. Furthermore, a linear discriminant analysis suggested three specific parameters to objectively classify any microglia by a decision tree. In addition, a principal components analysis revealed two extra valuable variables that allowed to further classifying microglia in a total of eight sub-clusters or types. The spatio-temporal distribution of these different morphotypes in our rat inflammation model allowed to relate specific morphotypes with microglial activation status and brain location. An objective method for microglia classification based on morphological parameters is proposed.
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Upon LPS/ATP treatment, iMG infused into tubular organoids displayed lower complexity in patterns and a spherical shape, exemplified by lower fractal dimensions, lower lacunarity, and higher convex-hull circularity.
“We found that, upon LPS/ATP treatment, iMG infused into our tubular organoids displayed a lower complexity in patterns as well as a spherical shape, exemplified by lower fractal dimensions, lower lacunarity, and higher convex-hull circularity (Figure 6c).56”
From Tubular human brain organoids to model microglia-mediated neuroinflammation (2021) · cites this paper as [56]
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- Authors
- Fernández-Arjona, M. D. M., Grondona, J. M., Granados-Durán, P., Fernández-Llebrez, P., & López-Ávalos, M. D.
- Affiliations
- Instituto de Investigación Biomédica de Málaga (ES)
- Corresponding author
- López-Ávalos, María D.
- Venue
- Frontiers in Cellular Neuroscience
- Publisher
- Frontiers Media SA
- Published
- 2017-08-08
- Volume
- 11
- Pages
- 235-235
- ISSN
- 1662-5102 (ISSN-L)
- PMC
- PMC5550745
- OpenAlex
- W2742827847
- Cited by
- 417
- References
- 61
- Citations by year
- 2026: 26 · 2025: 47 · 2024: 52 · 2023: 46 · 2022: 72 · 2021: 81 · 2020: 56 · 2019: 20 · 2018: 16 · 2013: 1
- FWCI
- 12.53
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Frontiers in Cellular Neuroscience (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RISalud Institutional Health Repository of Andalucía (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Consejería de Economía, Innovación, Ciencia y Empleo, Junta de Andalucía (P11-CVI-07637)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- microglial morphology, Iba1, fractal analysis, IL-1β, microglial activation states, hippocampus, hypothalamus, rat model
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