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Cited by 14Spaceflight effects on biologyTelomeres, Telomerase, and SenescencePlant Stress Responses and Tolerance

Telomere dynamics and oxidative stress in Arabidopsis grown in lunar regolith simulant

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Barcenilla, Borja Barbero, Kundel, Ishan, Hall, Emily, Hilty, Nicolas, Ulianich, Pavel, Cook, Jillian, Turley, Jake, Yerram, Monisha +3 more·2024·Frontiers in Plant Science
DOI 10.3389/fpls.2024.1351613PMC PMC10908177
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Abstract

NASA envisions a future where humans establish a thriving colony on the Moon by 2050. Plants will be essential for this endeavor, but little is known about their adaptation to extraterrestrial bodies. The capacity to grow plants in lunar regolith would represent a major step towards this goal by minimizing the reliance on resources transported from Earth. Recent studies reveal that Arabidopsis thaliana can germinate and grow on genuine lunar regolith as well as on lunar regolith simulant. However, plants arrest in vegetative development and activate a variety of stress response pathways, most notably the oxidative stress response. Telomeres are hotspots for oxidative damage in the genome and a marker of fitness in many organisms. Here we examine A. thaliana growth on a lunar regolith simulant and the impact of this resource on plant physiology and on telomere dynamics, telomerase enzyme activity and genome oxidation. We report that plants successfully set seed and generate a viable second plant generation if the lunar regolith simulant is pre-washed with an antioxidant cocktail. However, plants sustain a higher degree of genome oxidation and decreased biomass relative to conventional Earth soil cultivation. Moreover, telomerase activity substantially declines and telomeres shorten in plants grown in lunar regolith simulant, implying that genome integrity may not be sustainable over the long-term. Overcoming these challenges will be an important goal in ensuring success on the lunar frontier.

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  • Growth rates in regolith simulants tend to be slower and plants often exhibit signs of phenotypic stress.

    “However, growth rates tend to be slower, and plants often exhibit signs of phenotypic stress (Paul et al., 2022), including a decline in telomere dynamics and a loss of sustainable genome integrity (Barcenilla et al., 2024).”

    From Spirulina supported plant growth in regolith simulants and elevated levels of CO2 (2025) · cites this paper as Barcenilla et al., 2024

    The Results report shorter telomeres and reduced telomerase activity in Arabidopsis grown in lunar regolith simulant. The paper describes long-term genome integrity as potentially unsustainable, not as an observed loss. The slower growth and phenotypic stress in the citing sentence are attributed to Paul et al. (2022), not to this source. (LLM, conf=0.86)

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Authors
Barcenilla, B. B., Kundel, I., Hall, E., Hilty, N., Ulianich, P., Cook, J., Turley, J., Yerram, M., Min, J.-H., Castillo-González, C., & Shippen, D. E.
Affiliations
Texas A&M University (US)
Corresponding author
Shippen, Dorothy E.
ORCID
Venue
Frontiers in Plant Science
Publisher
Frontiers Media SA
Published
2024-02-16
Volume
15
Pages
1351613-1351613
ISSN
1664-462X (ISSN-L)
DOI
10.3389/fpls.2024.1351613
PMC
PMC10908177
OpenAlex
W4391881534
Cited by
14
References
8
Citations by year
2026: 10 · 2025: 4
FWCI
3.62
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Plant Science (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • National Aeronautics and Space Administration (80NSSC23K0302)
  • Nuclear Safety and Security Commission
  • National Institutes of Health
  • National Institute of General Medical Sciences (R01GM065383)
Topics
  • Health Sciences › Medicine › Physiology › Spaceflight effects on biology
  • Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
Keywords
lunar regolith simulant, Arabidopsis thaliana, telomeres, telomere shortening, telomerase activity, oxidative stress, lunar agriculture, antioxidant pretreatment, genome stability

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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