Crop growth and viability of seeds on Mars and Moon soil simulants
Open accessAbstract
If humans are going to establish a base on the Moon or on Mars they will have to grow their own crops. An option is to use Lunar and Martian regolith. These regoliths are not available for plant growth experiments, therefore NASA has developed regolith simulants. The major goal of this project was to cultivate and harvest crops on these Mars and Moon simulants. The simulants were mixed with organic matter to mimic the addition of residues from earlier harvests. Ten different crops, garden cress, rocket, tomato, radish, rye, quinoa, spinach, chives, pea and leek were sown in random lines in trays. Nine of the ten species grew well with the exception of spinach. It was possible to harvest edible parts for nine out of ten crops. The total biomass production per tray was highest for the Earth control and Mars soil simulant and differed significantly from Moon soil simulant. The seeds produced by three species were tested for germination (radish, rye and cress). The germination on Moon soil simulant was significantly lower in radish than for the Earth control soil.
AI snapshot
Written from the paper’s full text.
TL;DR
Ten crop species were grown on NASA Mars and Moon regolith simulants amended with organic matter and a nutrient solution, with Earth potting soil as control. Nine species produced edible parts, but total biomass was lower on Moon simulant and radish seed germination was significantly reduced on Moon simulant compared with Earth control.
Key findings
- Nine of the ten species grew well; spinach was the exception, flowering after only a few small leaves. Edible parts were harvested for nine out of ten crops.
- Total aboveground dry biomass was highest on Earth control and lowest on Moon simulant; Moon was significantly lower than Earth control (p=0.0318), while Mars simulant was not significantly different from either due to high variation among replicates.
- Seeds of radish, rye, and cress were tested. Rye seeds germinated on all media; radish germination on Moon simulant was significantly lower than Earth control, while Mars was not significantly different from either; cress seed weight was higher on Earth control than both simulants but differences were not statistically significant.
Methods
- Greenhouse experiment in Wageningen under Earth atmosphere, gravity, and light; average 21.1 ± 3.0 °C and 65.0 ± 15.5% humidity, with 16 h daylight; three replicate trays per treatment (n=3) for Mars simulant JSC-1A Mars-1A, Moon simulant JSC1-1A, and Earth organic soil control.
- Ten crops were sown in randomized lines in trays; simulants were mixed with 267 g fresh *Lolium perenne* grass per 7.5 L simulant, and a nutrient solution was added monthly except the first month; sowing was 9 April 2015 and harvest occurred 159 days later.
- Aboveground biomass was dried 48 h at 70°C and weighed; seeds of rye, cress, and radish were dried 48 h at 25°C, weighed, then sown 5 April 2016 for germination until 25 April 2016.
Limitations
- The paper states this was a limited preliminary experiment; peas were harvested as total biomass per treatment rather than per tray, so variance among the three replicates could not be evaluated, and missing pea replicate values were estimated based on variance of other species.
- The paper states species were grown together, so individual biomass per species could not be analyzed; it calls for further work on optimal organic matter, water-use efficiency, physical characteristics of simulants/regoliths, nutrient recycling, nitrogen fixation, and fungi.
- Evident from the text: water amount was not recorded; media and seeds were not sterilized; only three species were tested for germination; cress tray 3 had no seed setting; and spinach failed to grow properly.
Model-written summary. Check it against the paper before relying on it.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
5 of 20 cited sources checked · 3 supported · 0 flagged · accuracy not yet measured
- Findingown data§ 4 Discussion
Compared to earlier research (Wamelink et al. 2014), the biomass production was significantly higher.
“Compared to our earlier research (Wamelink et al. 2014), the biomass production was significantly higher, and crops such as tomato, rye, pea and radish formed fruit and viable seeds.”
Quote in the paperNot a substantial claim1 of 1 sources checked- cites Wamelink et al. 2014 source
The Results report biomass differences among soil types within the 2014 experiment, and the Statistical Analysis describes tests between those soil types. Neither section compares the 2014 biomass with the citing paper’s later experiment or establishes that the later production was significantly higher. The cited paper therefore provides an earlier measurement, but our copy cannot verify the cross-experiment claim. (LLM, conf=0.76)
Experimental — accuracy not yet measured
- Premisecitation§ 2.1 Regoliths
The pH of the Moon regolith simulant is 9.6.
“The pH of the Moon regolith simulant is so high (9.6,Wamelink et al. 2014)”
Quote in the paperNot a substantial claim1 of 1 sources checked- cites Wamelink et al. 2014 source
- Findingown data§ 3.2 Seed weight and germination
For three species, radish, cress and rye it was possible to harvest enough seeds for a germination experiment.
“For three species, radish, cress and rye it was possible to harvest enough seeds for a germination experiment.”
Quote in the paperSubstantial claim - Findingown data§ 3.2 Seed weight and germination
For cress, the seed weight for the Earth control is higher than for both simulants.
“For cress this looks different, the seed weight for the Earth control is higher than for both simulants.”
Quote in the paperNot a substantial claim - Findingown data§ Abstract
The germination on Moon soil simulant was significantly lower in radish than for the Earth control soil.
“The germination on Moon soil simulant was significantly lower in radish than for the Earth control soil.”
Quote in the paperSubstantial claim- The authors state this as an assumption: A balanced nutrient solution will be made based on faeces and urine.
- Findingown data§ 3.2 Seed weight and germination
The seed weight of radish and rye did not deviate between the three growing media.
“The seed weight of radish and rye did not deviate between the three growing media (Figure 3, for background data see Appendix 2).”
Quote in the paperNot a substantial claim - Findingown data§ 4 Discussion
None of the differences in seed weight of cress, radish and rye harvested from plants grown on Mars and Moon soil simulants and Earth potting soil are statistically significant.
“Seed weight of cress, radish and rye harvested from plants grown on Mars and Moon soil simulants and Earth potting soil.None of the differences are statistically significant (n=3, p=0.05, for cress due to the large uncertainty in the Earth control)”
Quote in the paperNot a substantial claim - Findingnone given§ 3.2 Seed weight and germination
None of the germinated plants died during the 20 days the germination experiment lasted.
“None of the germinated plants died during the 20 days the germination experiment lasted.”
Quote in the paperNot a substantial claim - Findingnone given§ 3.1 Fruit setting and biomass
There was high variation between the three replicas.
“also due to the high variation between the three replicas”
Quote in the paperSubstantial claim - Findingown data§ Abstract
The total biomass production per tray differed significantly from Moon soil simulant.
“The total biomass production per tray was highest for the Earth control and Mars soil simulant and differed significantly from Moon soil simulant.”
Quote in the paperSubstantial claim- The authors state this as an assumption: A balanced nutrient solution will be made based on faeces and urine.
- Findingnone given§ 4 Discussion
The biomass production on the Moon soil simulant was significantly lower than for the Earth soil.
“The biomass production on the Moon soil simulant was significantly lower than for the Earth soil.”
Quote in the paperSubstantial claim - Findingown data§ Abstract
The total biomass production per tray was highest for the Earth control and Mars soil simulant.
“The total biomass production per tray was highest for the Earth control and Mars soil simulant and differed significantly from Moon soil simulant.”
Quote in the paperSubstantial claim- The authors state this as an assumption: A balanced nutrient solution will be made based on faeces and urine.
- Findingown data§ 4 Discussion
The addition of organic matter to both the Mars and Moon regolith simulants resulted in the germination and growth of all ten crop species.
“The addition of organic matter to both the Mars and Moon regolith simulants resulted in the germination and growth of all ten crop species.”
Quote in the paperSubstantial claim - Findingown data§ 3.1 Fruit setting and biomass
Mars soil simulant was not significantly different from the Earth or Moon media soils in total aboveground biomass.
“However, Mars soil simulant was not significantly different from the Earth or Moon media soils, also due to the high variation between the three replicas.”
Quote in the paperNot a substantial claim - Findingown data§ 4 Discussion
Several crops produced fruits and seeds on both Mars and Moon simulants.
“Several crops produced fruits and seeds on both Mars and Moon simulants.”
Quote in the paperSubstantial claim - Findingown data§ 3.2 Seed weight and germination
None of the differences in seed weight between the three growing media are statistically significant (at p=0.05).
“However, none of the differences are statistically significant (at p=0.05).”
Quote in the paperNot a substantial claim - Findingnone given§ 4 Discussion
There was no seed setting for cress and radish in tray 3.
“There was no seed setting for cress and radish in tray 3.”
Quote in the paperNot a substantial claim - Findingnone given§ 4 Discussion
The biomass production on the Mars soil simulant was comparable to the Earth soil.
“The biomass production on the Mars soil simulant was comparable to the Earth soil.”
Quote in the paperSubstantial claim - Findingown data§ 4 Discussion
Crops such as tomato, rye, pea and radish formed fruit and viable seeds.
“Compared to our earlier research (Wamelink et al. 2014), the biomass production was significantly higher, and crops such as tomato, rye, pea and radish formed fruit and viable seeds.”
Quote in the paperSubstantial claim - Findingown data§ 3.1 Fruit setting and biomass
Chives and leek did grow steadily, but at a low growth rate on all three growing medias.
“Chives and leek did grow steadily, but at a low growth rate on all three growing medias”
Quote in the paperNot a substantial claim - Findingown data§ 3.2 Seed weight and germination
For rye all the seeds germinated on all three growing mediums.
“For rye all the seeds germinated on all three growing mediums (Figure 4).”
Quote in the paperNot a substantial claim - Findingown data§ 3.2 Seed weight and germination
The germination percentage was lowest for radish.
“The germination percentage was lowest for radish.”
Quote in the paperNot a substantial claim - Findingown data§ 3.1 Fruit setting and biomass
The harvested dry biomass on Moon soil simulant was lower than the Earth control (p=0.0318).
“The harvested dry biomass on Moon soil simulant was lower than the Earth control (p=0.0318).”
Quote in the paperSubstantial claim - Findingown data§ Abstract
It was possible to harvest edible parts for nine out of ten crops.
“It was possible to harvest edible parts for nine out of ten crops.”
Quote in the paperSubstantial claim- The authors state this as an assumption: A balanced nutrient solution will be made based on faeces and urine.
- Premisecitation§ 2.1 Regoliths
Mars and Moon regoliths are comparable to Earth soils, at least in mineral composition.
“Since the Mars and Moon regoliths are comparable to Earth soils, at least in mineral composition, they can be mimicked by using Earth soils, as has been done by NASA (Carlton et al. not dated;Chevrier and Mathe 2007;Clark 1993;Clark and Van Hart 1981;Gibson 1977;Rickman et al. 2007).”
Quote in the paperSubstantial claim0 of 3 sources checked- cites Carlton et al. not dated
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
- cites Chevrier and Mathe 2007
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
- cites Rickman et al. 2007
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
- Interpretationown data§ 4 Discussion
Crop growth on Mars and moon soil simulants is possible.
“In this limited preliminary experiment, we show that crop growth on Mars and moon soil simulants is possible.”
Quote in the paperSubstantial claim- Rests on an assumption: The Mars and Moon soil simulants used are representative of actual Martian and lunar regolith for crop growth.
- Interpretationown data§ 4 Discussion
For radish the viability of the seeds grown on Moon soil simulants was lower than on Earth soil.
“For radish the viability of the seeds grown on Moon soil simulants was lower than on Earth soil.”
Quote in the paperSubstantial claim - Interpretationown data§ 4 Discussion
Adding organic matter to the simulants improves plant growth.
“Now that we have shown that adding organic matter to the simulants improves plant growth one of the next steps will be to find the optimal amount of organic matter to add.”
Quote in the paperNot a substantial claim- Rests on an assumption: The improved plant growth on Earth potting soil compared to simulants is due to its organic matter content.
- Premisecitation§ 4 Discussion
The Mars regolith simulant JSC 1A resembles the actual Mars regolith closely.
“This simulant resembles the actual Mars regolith closely and originates from the Pu'u Nene cinder cone located between Mauna Loa and Mauna Kea on Hawaii (Rickman et al. 2007).”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites Rickman et al. 2007
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
- Interpretationown data§ 4 Discussion
For rye and cress the seeds are viable with almost no seeds that did not germinate.
“The results show that for rye and cress the seeds are viable with almost no seeds that did not germinate.”
Quote in the paperSubstantial claim - Interpretationcitation§ 2.1 Regoliths
The high pH of the Moon regolith simulant may be problematic for many plant species.
“that it may be problematic for many plant species (Wamelink et al. 2005).”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites Wamelink et al. 2005
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
- Classified as probably not a substantial claim. 17
- May combine two claims. 14
- The quote only partly states this claim. 1
Classified as probably not a substantial claim.
Premisecitation§ 1 Introduction14 different plant species grew successfully on Mars soil simulant and to a lesser extent on Moon soil simulant.
“After the successful growth of 14 different plant species on Mars soil simulant and to a lesser extent on Moon soil simulant (Wamelink et al. 2014)”
Quote in the paperSubstantial claim1 of 1 sources checked- cites Wamelink et al. 2014 source
May combine two claims.
Premisecitation§ 2.1 RegolithsThe Moon regolith simulant is nutrient poor, though it contained small amounts of nitrate and ammonium.
“The analysis revealed that the Moon regolith simulant is nutrient poor, though it contained small amounts of nitrate and ammonium.”
Quote in the paperNot a substantial claim1 of 1 sources checked- cites Wamelink et al. 2014 source
Table 2 (Materials and Methods, Regoliths; page not provided) reports 4.2 mg/kg of combined nitrate and nitrite in the lunar simulant, consistent with a small amount of available nitrogen. It also lists 0.3 mg/kg ammonium, but that is below the stated 1.0 mg/kg detection limit, so the analysis does not establish that ammonium was present. The Discussion describes the lunar simulant as lacking sufficient reactive nitrogen. (LLM, conf=0.86)
Experimental — accuracy not yet measured
May combine two claims.
Premisecitation§ 2.1 RegolithsThe Mars regolith simulant contained traces of nitrates and ammonium, and also a significant amount of carbon.
“The Mars regolith also contained traces of nitrates and ammonium, but also a significant amount of carbon.”
Quote in the paperNot a substantial claim1 of 1 sources checked- cites Wamelink et al. 2014 source
May combine two claims.
Findingown data§ 2.1 RegolithsWater holding capacity of the soils was around 30% for the Mars and Moon simulant and 100% for Earth organic soil, in weight percent.
“Water holding capacity of the soils was estimated and was around 30% for the Mars and Moon simulant and 100% for Earth organic soil, all in weight percent.”
Quote in the paperNot a substantial claimMay combine two claims.
Findingown data§ 3.2 Seed weight and germinationThere is a significant difference in germination for the seeds on Earth soil and Moon soil simulant for radish, with a higher percentage for seeds grown on Earth soil.
“There is a significant difference in germination for the seeds on Earth soil and Moon soil simulant for radish, with a higher percentage for seeds grown on Earth soil.”
Quote in the paperSubstantial claimMay combine two claims.
Findingown data§ 3.1 Fruit setting and biomassThe only species that did not grow properly on any of the soils was spinach; the plants started to flower after only a few small leaves were formed.
“The only species that did not grow properly on any of the soils was spinach; the plants started to flower after only a few small leaves were formed.”
Quote in the paperNot a substantial claimMay combine two claims.
Findingown data§ 3.1 Fruit setting and biomassWe were able to harvest radishes and radish seeds, cress and cress seeds, rye seeds, rocket, tomatoes and peas on Mars and Moon soil simulants and Earth organic soil.
“We were able to harvest radishes and radish seeds, cress and cress seeds, rye seeds, rocket, tomatoes and peas on Mars and Moon soil simulants and Earth organic soil.”
Quote in the paperSubstantial claimMay combine two claims.
Findingown data§ 3.1 Fruit setting and biomassThe total aboveground biomass was highest for the Earth control and lowest for the Moon soil simulant.
“The total aboveground biomass was highest for the Earth control and lowest for the Moon soil simulant (Figure 2).”
Quote in the paperNot a substantial claimMay combine two claims.
Findingnone given§ 3.1 Fruit setting and biomassQuinoa was growing well and formed flowers, but did not form any seeds.
“Quinoa was growing well and formed flowers, but did not form any seeds.”
Quote in the paperNot a substantial claimMay combine two claims.
Findingown data§ AbstractNine of the ten species grew well, with the exception of spinach.
“Nine of the ten species grew well with the exception of spinach.”
Quote in the paperSubstantial claim- The authors state this as an assumption: A balanced nutrient solution will be made based on faeces and urine.
May combine two claims.
Findingown data§ 2.1 RegolithsThere is only a small amount of reactive nitrogen present in the simulants and the pH of the simulants is high.
“We found that there is only a small amount of reactive nitrogen present in the simulants and that the pH of the simulants is high.”
Quote in the paperSubstantial claimProbably not a substantial claim: may not pass S3 (load-bearing).
Findingnone given§ 3.1 Fruit setting and biomassOne of the Mars trays, on the upper right corner of 3*3 lay out of the trays, had a much lower biomass.
“One of the Mars trays, on the upper right corner of 3*3 lay out of the trays, had a much lower biomass.”
Quote in the paperNot a substantial claimMay combine two claims.
Findingown data§ 3.2 Seed weight and germinationThe germination percentage of radish on Mars soil simulant was not significantly different from either the Earth control or Moon soil simulant (at p=0.05).
“The germination percentage of radish on Mars soil simulant was not significantly different from either the Earth control or Moon soil simulant (at p=0.05).”
Quote in the paperNot a substantial claimProbably not a substantial claim: may not pass S2 (checkable).
Premisecitation§ 1 IntroductionFor a permanent stay, production of crops on Mars or the Moon to supplement or supply the total food demand could be a necessity.
“Food can and will be brought along, but for a permanent stay, production of crops on Mars or the Moon to supplement or even supply the total food demand could be a necessity (Graham and Bamsey 2016).”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites Graham and Bamsey 2016
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
Classified as probably not a substantial claim.
Premisecitation§ 1 IntroductionEnsuring food availability and safety will be one of the major issues for a permanent human settlement on Mars or the Moon.
“One of the major issues will be ensuring food availability and safety (Cousins and Cockell 2016).”
Quote in the paperSubstantial claim0 of 1 sources checked- cites Cousins and Cockell 2016
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
Probably not a substantial claim: may not pass S4 (based).
Premisecitation§ AbstractIf humans establish a permanent base on the Moon or Mars, they will have to grow their own crops.
“If humans are going to establish a base on the Moon or on Mars they will have to grow their own crops.”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites Graham and Bamsey 2016
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
May combine two claims.
Premisecitation§ 1 IntroductionThe JSC 1A Moon simulant resembles the actual Moon regolith closely; no important deviations from the original regolith are reported.
“It resembles the actual Moon regolith closely; no important deviations from the original regolith are reported as far as we know.”
Quote in the paperSubstantial claim0 of 1 sources checked- cites Rickman et al. 2007
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
Probably not a substantial claim: may not pass S3 (load-bearing). May combine two claims.
Study design and conductnone given§ 2.4 Experimental designThe order of the species was randomized in a split plot design, resulting in a different order of the species for each tray.
“The ten crops were sown in a line, the order of the species was randomized as well in a split plot design, resulting in a different order of the species for each tray.”
Quote in the paperNot a substantial claimProbably not a substantial claim: may not pass S3 (load-bearing).
Study design and conductown data§ 2.4 Experimental designThere were three replicates in the experiment (n=3).
“There were three replicates in the experiment (n=3), i.e. three trays (with punctures in the bottom) with Mars soil simulant, three trays with Moon soil simulant and three trays with the control, Earth potting soil”
Quote in the paperNot a substantial claimProbably not a substantial claim: may not pass S3 (load-bearing).
Study design and conductnone given§ 2.4 Experimental designThe amount of water given was not recorded.
“The amount of water given was not recorded.”
Quote in the paperNot a substantial claimThe quote only partly states this claim.
Study design and conductown data§ 4 DiscussionIt was not possible to evaluate the variance between the three replicas for peas.
“Therefore, it was not possible to evaluate the variance between the three replicas.”
Quote in the paperNot a substantial claimProbably not a substantial claim: may not pass S3 (load-bearing).
Study design and conductnone given§ 4 DiscussionThe peas were harvested as a total biomass per treatment and not per tray.
“Unfortunately, the peas were harvested as a total biomass per treatment and not per tray.”
Quote in the paperNot a substantial claimProbably not a substantial claim: may not pass S3 (load-bearing).
Study design and conductreasoning§ 4 DiscussionTo overcome the problem of missing replicates, we estimated the values for the peas based on the variance of the other species.
“To overcome the problem of missing replicates, we estimated the values for the peas based on the variance of the other species.”
Quote in the paperNot a substantial claimProbably not a substantial claim: may not pass S3 (load-bearing).
Premisecitation§ 2.2 Species selectionSeveral of the selected crops are included by Perchonok and Bourland (2002) as part of a diet for a long stay in space.
“Several of our selected crops are also included by Perchonok and Bourland (2002) as part of a diet for a long stay in space.”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites Perchonok and Bourland 2002 source
Probably not a substantial claim: may not pass S3 (load-bearing).
Study design and conductnone given§ 2.5 Harvest and measurementsWe duplicated the same random design for the trays and the sequence of species per tray as in 2015, with exclusion of the other six species.
“We duplicated the same random design for the trays and the sequence of species per tray as in 2015, with exclusion of the other six species.”
Quote in the paperNot a substantial claimProbably not a substantial claim: may not pass S3 (load-bearing).
Study design and conductreasoning§ 2.4 Experimental designThe experiment used a randomized complete block design for the total biomass harvest.
“This results in a randomized complete block design for the total biomass harvest.”
Quote in the paperNot a substantial claimProbably not a substantial claim: may not pass S3 (load-bearing).
Premisecitation§ 2.2 Species selectionAstronauts on the ISS often complain about the taste of their food.
“Astronauts on the ISS often complain about the taste of their food and spicy crops may therefore be a welcome addition to their diet (Cooper et al. 2011).”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites Cooper et al. 2011 source
Probably not a substantial claim: may not pass S2 (checkable), S3 (load-bearing).
Interpretationcitation§ 2.2 Species selectionSpicy crops may be a welcome addition to astronauts' diet.
“spicy crops may therefore be a welcome addition to their diet (Cooper et al. 2011).”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites Cooper et al. 2011 source
Probably not a substantial claim: may not pass S3 (load-bearing).
Study design and conductreasoning§ 4 DiscussionThis had consequences for the evaluation of the total biomass.
“This had consequences for the evaluation of the total biomass as well.”
Quote in the paperNot a substantial claimProbably not a substantial claim: may not pass S3 (load-bearing).
Premisecitation§ 4 DiscussionEarlier research on Arabidopsis thaliana and Tagetes patula on regolith and Moon rock simulant also produced flowering plants.
“Earlier research on Arabidopsis thaliana (L.) Heynh.and Tagetes patula L. (Ferl et al. 2010; Kozyrovska et al. 2006; Zaets et al. 2011) on regolith and Moon rocksimulant also produced flowering plants.”
Quote in the paperNot a substantial claim0 of 3 sources checkedMay combine two claims.
Interpretationown data§ 4 DiscussionThe seed weight is reasonable for all of the four tested species, since there are no significant differences between the soils.
“A quality indicator is the seed weight and that is reasonable for all of the four tested species, since there are no significant differences between the soils.”
Quote in the paperNot a substantial claim- Rests on an assumption: Seed weight is a quality indicator for seed viability.
The authors state this as an assumption.
Plants will be grown indoors and below ground on Mars or the Moon to avoid the unfavourable conditions outside.
“We assume that plants will be grown indoors and below ground on Mars or the Moon to avoid the unfavourable conditions outside.”
The authors state this as an assumption.
A balanced nutrient solution will be made based on faeces and urine.
“We assumed that based on the faeces and urine a balanced solution will be made”
The authors state this as an assumption.
Bacteria will be brought to Mars and the Moon to support plant growth and organic matter breakdown.
“We assume that bacteria will be brought to Mars and the Moon to support plant growth and organic matter break down.”
The paper relies on this without support.
Moon regolith is not available for growing plants at sufficient quantities at the moment.
“However, Moon regolith is not available for growing plants at sufficient quantities at the moment.”
The paper relies on this without support.
Seed production is necessary to prevent new seeds from being flown in for every generation.
“Seed production is a necessity to prevent new seeds to be flown in for every generation.”
The paper relies on this without support.
The formed seeds must be viable.
“The formed seeds have to be viable.”
The paper relies on this without support.
Seed weight is a quality indicator for seed viability.
“A quality indicator is the seed weight”
The paper relies on this without support.
The Mars and Moon soil simulants used are representative of actual Martian and lunar regolith for crop growth.
“crop growth on Mars and moon soil simulants is possible”
The paper relies on this without support.
The Earth potting soil used as control is a suitable reference for fertile growth medium.
“three trays with the control, Earth potting soil”
The paper relies on this without support.
The improved plant growth on Earth potting soil compared to simulants is due to its organic matter content.
“Now that we have shown that adding organic matter to the simulants improves plant growth”
The paper relies on this without support.
Mars regolith is not yet available on Earth.
“Mars regolith is not yet available on Earth.”
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In Wamelink et al. (2019), only three species (radish, rye, and cress) produced seeds.
“Only three species (radish, rye, and cress) produced seeds.”
From The Potential for Lunar and Martian Regolith Simulants to Sustain Plant Growth: A Multidisciplinary Overview (2022) · cites this paper as Wamelink et al., 2019
Section 2.5 says seeds were harvested from radish, rye, and cress for the germination experiment, but that does not mean they were the only species to produce seeds. The Discussion also says tomato and pea formed fruit and viable seeds, contrary to the claim’s “only three.” (LLM, conf=0.86)
Checked by a reasoning model — may be wrong.
Experimental — accuracy not yet measuredIn Wamelink et al. (2019), radish germination rates were lower in the Lunar simulant than in the Earth control soil and Martian simulant.
“Radish germination rates were lower in the Lunar simulant than in the Earth control soil and Marian simulant.”
From The Potential for Lunar and Martian Regolith Simulants to Sustain Plant Growth: A Multidisciplinary Overview (2022) · cites this paper as Wamelink et al., 2019
Section 3.2 reports significantly higher radish-seed germination for seeds grown in Earth soil than for those grown in Moon soil simulant. It does not state that the Moon-simulant rate was lower than the Mars-simulant rate; it says the Mars rate was not significantly different from either. The Mars comparison is therefore not established by the text of our copy. (LLM, conf=0.85)
Experimental — accuracy not yet measuredIn Wamelink et al. (2019), biomass production was highest in the Earth control and the Martian soil simulant, but was significantly lower in the Lunar simulant.
“The biomass production was highest in the Earth control and the Martian soil simulant, but was significantly lower in the Lunar simulant.”
From The Potential for Lunar and Martian Regolith Simulants to Sustain Plant Growth: A Multidisciplinary Overview (2022) · cites this paper as Wamelink et al., 2019
Section 3.1 (Fruit setting and biomass) reports that total aboveground biomass was highest in the Earth control and lowest in the Moon simulant, with Moon dry biomass significantly below Earth (p=0.0318). It also states that Mars biomass was not significantly different from either Earth or Moon, so the claim is unsupported if it implies a significant Mars–Moon difference. (LLM, conf=0.90)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
Each contradiction between two passages listed here was confirmed by an independent check.
Discussion refers to four seed-weight species, but lists three
MinorThe discussion calls seed weight a quality indicator for four tested species, whereas the seed-weight figure identifies only cress, radish and rye.
“A quality indicator is the seed weight and that is reasonable for all of the four tested species, since there are no significant differences between the soils.”
“Figure 3: Seed weight of cress, radish and rye harvested from plants grown on Mars and Moon soil simulants and Earth potting soil.”
Germination experiment excludes seven species, not six
MinorThe original experiment sowed ten species, while the germination experiment included three. That leaves seven species excluded, not six.
Reported 6 · recomputed 7
“We duplicated the same random design for the trays and the sequence of species per tray as in 2015, with exclusion of the other six species.”
Moon-grown cress had substantial germination failure
MinorThe discussion says almost no rye or cress seeds failed to germinate, but the Moon-grown cress entries show 7 and 5 germinations out of 10 each. Eight of those 20 cress seeds failed to germinate.
“The results show that for rye and cress the seeds are viable with almost no seeds that did not germinate.”
“L1 25 0.048 0.00192 10 7 L2 25 0.055 0.0022 10 5”
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- Authors
- Wamelink, G. W. W., Frissel, J. Y., Krijnen, W. H. J., & Verwoert, M. R.
- Affiliations
- Wageningen University & Research (NL)
- Corresponding author
- Wamelink, G.W.W.
- ORCID
- Wamelink, G.W.W.
- Venue
- Open Agriculture
- Publisher
- Walter de Gruyter GmbH
- Published
- 2019-01-01
- Volume
- 4
- Issue
- 1
- Pages
- 509-516
- ISSN
- 2391-9531 (ISSN-L)
- OpenAlex
- W2978622258
- Semantic Scholar
- 39d69243e3d50d215e6720e358d4cc247c877137
- Cited by
- 62
- References
- 22
- Citations by year
- 2026: 7 · 2025: 12 · 2024: 14 · 2023: 6 · 2022: 12 · 2021: 9 · 2020: 2
- FWCI
- 3.07
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Open Agriculture (publishedVersion, cc-by-nc-nd)
- Socio-Environmental Systems Modeling (acceptedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion, cc-by-nc-nd)
- Funders
- Wageningen University and Research
- Topics
- Physical Sciences › Physics and Astronomy › Astronomy and Astrophysics › Planetary Science and Exploration
- Life Sciences › Agricultural and Biological Sciences › Soil Science › Plant Growth and Agriculture Techniques
- Physical Sciences › Engineering › Aerospace Engineering › Space Exploration and Technology
- Keywords
- lunar regolith, Martian regolith, crop growth, seed germination, space agriculture, biomass production, Lepidium sativum, radish, rye, spinach, food crops, organic amendments
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