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Cited by 34Bee Products Chemical AnalysisWound Healing and TreatmentsHealthcare and Venom Research

Histological, Immunohistochemical and Antioxidant Analysis of Skin Wound Healing Influenced by the Topical Application of Brazilian Red Propolis

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Conceição, Mariana, Gushiken, Lucas Fernando Sérgio, Aldana-Mejía, Jennyfer Andrea, Tanimoto, Matheus Hikaru, Ferreira, Marcos Vital de Sá, Alves, Andreia Cristina Miranda, Miyashita, Marina Naomi, Bastos, Jairo Kenupp +2 more·2022·Antioxidants
DOI 10.3390/antiox11112188PMC PMC9686645
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Abstract

Skin wound healing is a complex process that requires the mutual work of cellular and molecular agents to promote tissue restoration. In order to improve such a process, especially in cases of impaired healing (e.g., diabetic ulcer, chronic wounds), there is a search for substances with healing properties and low toxicity: two features that some natural products—such as the bee product named propolis—exhibit. Propolis is a resinous substance obtained from plant resins and exudates with antioxidant, anti-inflammatory, and antitumoral activities, among other biological ones. Based on the previously reported healing actions of different types of propolis, the Brazilian red propolis (BRP) was tested for this matter. A skin wound excision model in male Wistar rats was performed using two topical formulations with 1% red propolis as treatments: hydroalcoholic extract and Paste. Macroscopical, histological and immunohistochemical analysis were performed, revealing that red propolis enhanced wound contraction, epithelialization, reduced crust formation, and modulated the distribution of healing associated factors, mainly collagen I, collagen III, MMP-9, TGF-β3 and VEGF. Biochemical analysis with the antioxidants SOD, MPO, GSH and GR showed that propolis acts similarly to the positive control, collagenase, increasing these molecules’ activity. These results suggest that BRP promotes enhanced wound healing by modulating growth factors and antioxidant molecules related to cutaneous wound healing.

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  • Propolis has been shown to reduce scar formation, shorten healing time, increase wound contraction, enhance epithelialization, and accelerate tissue repair.

    “Propolis has been shown to reduce scar formation, shorten healing time, increase wound contraction, enhance epithelialization, and accelerate tissue repair [24,36,54].”

    From Anti-Inflammatory and Histological Analysis of Skin Wound Healing through Topical Application of Mexican Propolis (2023) · cites this paper as [54]

    The Results (section 3.1; page unavailable) report better epithelialization with one Brazilian red propolis formulation, supporting that part of the claim. They also report no significant difference in wound contraction versus untreated wounds. The Discussion (section 4; page unavailable) describes reduced scarring only as a possibility requiring confirmation, and the paper does not establish a shorter healing time. (LLM, conf=0.91)

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Authors
Conceição, M., Gushiken, L. F. S., Aldana-Mejía, J. A., Tanimoto, M. H., Ferreira, M. V. D. S., Alves, A. C. M., Miyashita, M. N., Bastos, J. K., Beserra, F. P., & Pellizzon, C. H.
Affiliations
  • Universidade Estadual Paulista (Unesp) (BR)
  • School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto 14040-903, SP, Brazil
  • Universidade de São Paulo (BR)
Corresponding author
  • Conceição, Mariana
  • Pellizzon, Cláudia Helena
ORCID
Venue
Antioxidants
Publisher
MDPI AG
Published
2022-11-04
Volume
11
Issue
11
Pages
2188
ISSN
2076-3921 (ISSN-L)
DOI
10.3390/antiox11112188
PMC
PMC9686645
OpenAlex
W4308443703
Cited by
34
References
2
Citations by year
2026: 9 · 2025: 12 · 2024: 9 · 2023: 4
FWCI
4.55
Type
journal article
Language
en
Open access
Licence
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  • Antioxidants (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
São Paulo Research Foundation (2021/01258-8, 2017/04138-8)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Bee Products Chemical Analysis
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
  • Health Sciences › Medicine › Pharmacology › Healthcare and Venom Research
Keywords
Brazilian red propolis, skin wound healing, topical application, excision wound model, type I collagen, type III collagen, MMP-9, TGF-beta3, vascular endothelial growth factor, antioxidant enzymes, superoxide dismutase, glutathione reductase, wound contraction, re-epithelialization, chronic wounds, diabetic ulcers

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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