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Cited by 28Wound Healing and TreatmentsPeriodontal Regeneration and TreatmentsHydrogels: synthesis, properties, applications

A Biomimetic Polynucleotides–Hyaluronic Acid Hydrogel Promotes Wound Healing in a Primary Gingival Fibroblast Model

Open access
Colangelo, Maria Teresa, Belletti, Silvana, Govoni, Paolo, Guizzardi, Stefano, Galli, Carlo·2021·Applied Sciences
DOI 10.3390/app11104405
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Abstract

Polynucleotides (PN) have long been known as an effective supportive therapy for wound healing. The present study investigated whether a hydrogel formulation containing PN and hyaluronic acid (PN + HA) could promote wound healing in an in vitro model of gingival fibroblasts. PN promoted cell growth and viability as assessed by different assays, and PN + HA, though not significantly further increasing cell growth as compared to PN, supported the formation of dense multilayered cell nodules. PN promoted fibroblasts’ clonogenic efficiency and PN + HA further enhanced the formation of more numerous dense colonies. PN + HA appeared to significantly increase the expression of collagen 1a1 and 3a1, while not affecting proteoglycans deposition. Interestingly, when tested in a scratch assay, PN + HA achieved gap closure after 48 h, while cells in the comparison groups had not completely bridged the scratch even after 96 h. Taken together, these results demonstrate that PN + HA is a promising candidate for a supportive therapy to promote soft tissue healing in the oral cavity.

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  • PN has shown benefits for skin health, including improving skin texture and elasticity, and reducing wrinkle depth.

    “In dermatology, PN, primarily derived from fish testes, has shown promising benefits for skin health, including improving skin texture and elasticity, and reducing wrinkle depth [26,27,28].”

    From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [26]

    The Materials section identifies salmon trout gonads as the source of the PN used, and the Introduction describes PN use in dermal fibroblast models and skin wound healing. Neither those passages nor the passages found by searching for texture, elasticity, and wrinkles report improvements in skin texture or elasticity or reduced wrinkle depth; the study itself uses a gingival fibroblast model. (LLM, conf=0.82)

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Cites

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Research works printed but not linked: 26

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Cited by

Sorted by citations

Authors
Colangelo, M. T., Belletti, S., Govoni, P., Guizzardi, S., & Galli, C.
Affiliations
University of Parma (IT)
Corresponding author
Galli, Carlo
ORCID
Venue
Applied Sciences
Publisher
MDPI AG
Published
2021-05-12
Volume
11
Issue
10
Pages
4405
ISSN
2076-3417 (ISSN-L)
DOI
10.3390/app11104405
OpenAlex
W3160237400
Cited by
28
References
3
Citations by year
2026: 6 · 2025: 13 · 2024: 3 · 2023: 4 · 2022: 1 · 2021: 1
FWCI
1.19
Type
journal article
Language
en
Open access
Licence
Locations
  • Applied Sciences (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
  • Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
Keywords
polynucleotides, hyaluronic acid, hydrogels, wound healing, gingival fibroblasts, collagen expression, cell proliferation, clonogenic efficiency, biomimetic biomaterials

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