Cited by 114Redox biology and oxidative stressAdvanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and Applications

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Mass Spectrometry-Based Methods for Identifying Oxidized Proteins in Disease: Advances and Challenges

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Verrastro, Ivan, Pasha, Sabah, Jensen, Karina, Pitt, Andrew, Spickett, Corinne·2015·Biomolecules

DOI 10.3390/biom5020378PMC PMC4496678

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Abstract

Many inflammatory diseases have an oxidative aetiology, which leads to oxidative damage to biomolecules, including proteins. It is now increasingly recognized that oxidative post-translational modifications (oxPTMs) of proteins affect cell signalling and behaviour, and can contribute to pathology. Moreover, oxidized proteins have potential as biomarkers for inflammatory diseases. Although many assays for generic protein oxidation and breakdown products of protein oxidation are available, only advanced tandem mass spectrometry approaches have the power to localize specific oxPTMs in identified proteins. While much work has been carried out using untargeted or discovery mass spectrometry approaches, identification of oxPTMs in disease has benefitted from the development of sophisticated targeted or semi-targeted scanning routines, combined with chemical labeling and enrichment approaches. Nevertheless, many potential pitfalls exist which can result in incorrect identifications. This review explains the limitations, advantages and challenges of all of these approaches to detecting oxidatively modified proteins, and provides an update on recent literature in which they have been used to detect and quantify protein oxidation in disease.

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Figure 1 Structures of oxidized residues most commonly detected and studied by mass spectrometry. In mixed disulfides, R can be cysteine or glutathione (glutathionylation).CC BY · PubMed Central

Figure 2 Summary of advanced methods for identification of proteins and oxPTMs. Labeling and enrichment can also be carried out at the protein level, but this approach is less common.CC BY · PubMed Central

Figure 3 Incorrectly assigned oxidation to proline using a probability-based search engine. (a) Search engine identified 2 modifications on one peptide: methionine-7 mono-oxidation and proline-9 oxidation; (b) de novo sequencing showed that methionine is dioxidised.CC BY · PubMed Central

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Authors: Verrastro, I., Pasha, S., Jensen, K., Pitt, A., & Spickett, C.
Affiliations: Aston University (GB)
Corresponding author: Spickett, Corinne
ORCID: Pitt, Andrew
Spickett, Corinne
Venue: Biomolecules
Publisher: MDPI AG
Published: 2015-04-14
Volume: 5
Issue: 2
Pages: 378-411
ISSN: 2218-273X (ISSN-L)
DOI: 10.3390/biom5020378
PMC: PMC4496678
OpenAlex: W2132553988
Cited by: 114
References: 189
Citations by year: 2026: 8 · 2025: 14 · 2024: 6 · 2023: 11 · 2022: 7 · 2021: 18 · 2020: 10 · 2019: 7 · 2018: 13 · 2017: 13 · 2016: 6
FWCI: 1.03
Type: journal article
Language: en
Open access: gold
dx.doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0/ (vor)
Locations: Biomolecules (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
Aston Publications Explorer (Aston University) (acceptedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Research Explorer (The University of Manchester) (submittedVersion)
Funders: EPSRC (EP/I017887/1)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
Keywords: oxidized proteins, oxidative post-translational modifications, protein oxidation, inflammatory diseases, biomarkers, tandem mass spectrometry, targeted mass spectrometry, chemical labeling, oxidative damage
MeSH terms: Animals, Cardiovascular Diseases, Humans, Oxidation-Reduction, Protein Processing, Post-Translational, Mass Spectrometry, Advanced Oxidation Protein Products

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Mass Spectrometry-Based Methods for Identifying Oxidized Proteins in Disease: Advances and Challenges

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