Binding of SU(VAR)3-9 partially depends on SETDB1 in the chromosomes of drosophila melanogaster
Open accessAbstract
H3K9 methylation is known to play a critical role in gene silencing. This modification is established and maintained by several enzymes, but relationships between them are not fully understood. In the present study, we decipher the interplay between two Drosophila H3K9-specific histone methyltransferases, SU(VAR)3-9 and SETDB1. We asked whether SETDB1 is required for targeting of SU(VAR)3-9. Using DamID-seq, we obtained SU(VAR)3-9 binding profiles for the chromosomes from larval salivary glands and germline cells from adult females, and compared profiles between the wild type and SETDB1-mutant backgrounds. Our analyses indicate that the vast majority of single copy genes in euchromatin are targeted by SU(VAR)3-9 only in the presence of SETDB1, whereas SU(VAR)3-9 binding at repeated sequences in heterochromatin is largely SETDB1-independent. Interestingly, piRNA clusters 42AB and 38C in salivary gland chromosomes bind SU(VAR)3-9 regardless of SETDB1, whereas binding to the same regions in the germline cells is SETDB1-dependent. In addition, we compared SU(VAR)3-9 profiles in female germline cells at different developmental stages (germarium cells in juvenile ovaries and mature nurse cells). It turned out that SU(VAR)3-9 binding is influenced both by the presence of SETDB1, as well as by the differentiation stage.
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Binding of Su(var)3-9 with chromatin partially depends on Egg in ovarian germ cells.
“and its binding with the chromatin partially depends on Egg in ovarian germ cells (106).”
From Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon (2022) · cites this paper as [106]
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- Authors
- Maksimov, D. A., & Koryakov, D. E.
- Affiliations
- Novosibirsk State University (RU)
- Institute of Molecular and Cell Biology (RU)
- Corresponding author
- Koryakov, Dmitry E.
- ORCID
- Koryakov, Dmitry E.
- Venue
- Cells
- Publisher
- MDPI AG
- Published
- 2019-09-05
- Volume
- 8
- Issue
- 9
- Pages
- 1030
- ISSN
- 2073-4409 (ISSN-L)
- PMC
- PMC6769583
- OpenAlex
- W2972284926
- Cited by
- 8
- References
- 46
- Citations by year
- 2026: 1 · 2023: 3 · 2022: 1 · 2021: 1 · 2020: 2
- FWCI
- 0.33
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- Cells (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Russian Foundation for Basic Research (19-04-00352)
- Russian Program for Basic Research (0310-2019-0003, 0310-2019-0005)
- Ministry of Education and Science of Russian Federation (#14.Y26.31.0024)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Keywords
- Su(var)3-9, SETDB1, H3K9 methylation, histone methyltransferase, protein binding sites, heterochromatin, euchromatin, piRNA clusters, germ cells, salivary gland chromosomes, gene silencing, Drosophila melanogaster, nurse cells
- MeSH terms
- Animals, Chromatin, Drosophila melanogaster, Female, Germ Cells, Ovary, Protein Binding, Histone-Lysine N-Methyltransferase, Repressor Proteins, Salivary Glands, Gene Expression Regulation, Developmental, Drosophila Proteins, RNA, Small Interfering
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