Exploring Genetic and Epigenetic Changes in Lingonberry Using Molecular Markers: Implications for Clonal Propagation
Open accessAbstract
Lingonberry (Vaccinium vitis-idaea L.) is an important and valuable horticultural crop due to its high antioxidant properties. Plant tissue culture is an advanced propagation system employed in horticultural crops. However, the progeny derived using this technique may not be true-to-type. In order to obtain the maximum return of any agricultural enterprise, uniformity of planting materials is necessary, which sometimes is not achieved due to genetic and epigenetic instabilities under in vitro culture. Therefore, we analyzed morphological traits and genetic and epigenetic variations under tissue-culture and greenhouse conditions in lingonberry using molecular markers. Leaf length and leaf width under greenhouse conditions and shoot number per explant, shoot height and shoot vigor under in vitro conditions were higher in hybrid H1 compared to the cultivar Erntedank. Clonal fidelity study using one expressed sequence tag (EST)—polymerase chain reaction (PCR), five EST—simple sequence repeat (SSR) and six genomic (G)—SSR markers revealed monomorphic bands in micropropagated shoots and plants in lingonberry hybrid H1 and cultivar Erntedank conforming genetic integrity. Epigenetic variation was studied by quantifying cytosine methylation using a methylation-sensitive amplification polymorphism (MSAP) technique. DNA methylation ranged from 32% in greenhouse-grown hybrid H1 to 44% in cultivar Erntedank under a tissue culture system. Although total methylation was higher in in vitro grown shoots, fully methylated bands were observed more in the greenhouse-grown plants. On the contrary, hemimethylated DNA bands were more prominent in tissue culture conditions as compared to the greenhouse-grown plants. The study conclude that lingonberry maintains its genetic integrity but undergoes variable epigenetic changes during in vitro and ex vitro conditions.
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In Vaccinium vitis-idaea micropropagated shoot tips, genetic equality was observed.
“Genetic equality observed.”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [129]
The Abstract reports monomorphic marker bands in micropropagated lingonberry shoots and plants, supporting genetic integrity in the material tested. The Materials and Methods (§2.1) say the cultures began from leaf explants and that shoots were sampled for DNA analysis; our copy does not establish the finding specifically for shoot tips. (LLM, conf=0.82)
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- Authors
- Sharma, U., Sikdar, A., Igamberdiev, A. U., & Debnath, S. C.
- Affiliations
- Memorial University of Newfoundland (CA)
- Agriculture and Agri-Food Canada (CA)
- Corresponding author
- Debnath, Samir C.
- Venue
- Current Issues in Molecular Biology
- Publisher
- MDPI AG
- Published
- 2023-07-28
- Volume
- 45
- Issue
- 8
- Pages
- 6296-6310
- ISSN
- 1467-3037 (ISSN-L)
- 1467-3045
- PMC
- PMC10453208
- OpenAlex
- W4385341248
- Cited by
- 8
- References
- 52
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 1
- FWCI
- 0.71
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Current Issues in Molecular Biology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Memorial University of Newfoundland
- Agriculture and Agri-Food Canada
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Berry genetics and cultivation research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- lingonberry, Vaccinium myrtillus, clonal fidelity, epigenetic variation, DNA methylation, cytosine methylation, micropropagation, in vitro culture, greenhouse cultivation, genetic stability, morphological variation, hybrid cultivars, EST-SSR markers, genomic SSR markers, hemimethylation
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