Exploring the Nutritional Potential of Microalgae in the Formulation of Bakery Products
Open accessAbstract
Microalgae have positioned themselves as an innovative and sustainable source of bioactive compounds and high nutritional value. The selection of a suitable food carrier is important to ease its consumption, and to preserve bioactivity through food processing. The aim of this study was to assess the suitability of different microalgae in baked products. Crackers and grissini were produced following a specific formulation, with percentages ranging from 1.5 to 3.5% of flour substituted with Spirulina, Chlorella, and Tetraselmis dry biomass in the formulas. Physico-chemical, nutritional, and sensorial characterization was carried out. The incorporation of microalgae led to increased nutritional values, including antioxidant capacity (AOX), total phenolic content (TPC) and protein content with an amino acids’ identification and quantification. Grissini with Chlorella at 3.5% and crackers with Spirulina at 1.5% levels, showed a higher overall acceptance within the panelists. For amino acid content, Spirulina crackers were shown to be rich in alanine, aspartate, and tryptophan, while Chlorella grissini stood out for being particularly rich in isoleucine, leucine, lysine, and valine. Thus, Spirulina and Chlorella could be a sustainable ingredient to formulate baked goods with an enhanced nutrimental matrix without altering their acceptability to consumers.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Spirulina incorporation in bakery products provides a nutritional boost in protein and fiber, enriches the diet with minerals, contributes color, and improves antioxidant activity.
“In addition to providing a nutritional boost in protein and fiber and enriching the diet with minerals [78,79], color contribution was also reported [79,80], and all these studies noted improved antioxidant activity associated with Spirulina incorporation.”
From Valorization of Microalgae as Multifunctional Protein Sources in Plant-Based Systems: Structural, Functional, and Processing Challenges (2026) · cites this paper as [80]
Section 3.2 (page unavailable) reports strong antioxidant activity for Spirulina crackers, and the Introduction describes Spirulina pigments as colorants. But Section 3.2 explicitly says Spirulina did not increase protein content in the crackers or grissini tested; the cited passages also do not establish increased fiber or mineral content in those products. (LLM, conf=0.89)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 315,771 · see citing works
Micro-algae as a source of protein
Becker, E. W. (2007) · Biotechnology Advances · doi:10.1016/j.biotechadv.2006.11.002 · Cited by 2,311 · see citing works
Microalgae as versatile cellular factories for valued products
Koller, M., Muhr, A., & Braunegg, G. (2014) · Algal Research · doi:10.1016/j.algal.2014.09.002 · Cited by 639 · see citing works
Antioxidant potential of microalgae in relation to their phenolic and carotenoid content
Goiris, K., Muylaert, K., Fraeye, I., Foubert, I., Brabanter, J. D., & Cooman, L. D. (2012) · Journal of Applied Phycology · doi:10.1007/s10811-012-9804-6 · Cited by 590 · see citing works
Wojtunik‐Kulesza, K. A., Oniszczuk, A., Oniszczuk, T., Combrzyński, M., Nowakowska, D., & Matwijczuk, A. P. (2020) · Nutrients · doi:10.3390/nu12051401 · Cited by 464 · see citing works
Microalgae for “Healthy” Foods-Possibilities and Challenges
Chacón‐Lee, T. L., & González‐Mariño, G. E. (2010) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/j.1541-4337.2010.00132.x · Cited by 428 · see citing works
Tibbetts, S. M., Milley, J. E., & Lall, S. P. (2014) · Journal of Applied Phycology · doi:10.1007/s10811-014-0428-x · Cited by 423 · see citing works
Microalgae, old sustainable food and fashion nutraceuticals
García, J. L., de Vicente, M., & Galán, B. (2017) · Microbial Biotechnology · doi:10.1111/1751-7915.12800 · Cited by 422 · see citing works
Batista, A. P., Niccolai, A., Fradinho, P., Fragoso, S., Bursic, I., Rodolfi, L., Biondi, N., Tredici, M. R., Sousa, I., & Raymundo, A. (2017) · Algal Research · doi:10.1016/j.algal.2017.07.017 · Cited by 407 · see citing works
Microalgae as Sources of High-Quality Protein for Human Food and Protein Supplements
Wang, Y., Tibbetts, S. M., & McGinn, P. J. (2021) · Foods · doi:10.3390/foods10123002 · Cited by 342 · see citing works
Microalgae–nutritious, sustainable aqua-and animal feed source
Dineshbabu, G., Goswami, G., Kumar, R., Sinha, A., & Das, D. (2019) · Journal of Functional Foods · doi:10.1016/j.jff.2019.103545 · Cited by 285 · see citing works
Effects of Spirulina Biomass on the Technological and Nutritional Quality of Bread Wheat Pasta
Rodríguez De Marco, E., Steffolani, M. E., Martínez, C. S., & León, A. E. (2014) · LWT - Food Science and Technology · doi:10.1016/j.lwt.2014.02.054 · Cited by 257 · see citing works
The Impact of Microalgae in Food Science and Technology
Matos, Â. P. (2017) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-017-3050-7 · Cited by 222 · see citing works
Microalgae as Functional Ingredients in Savory Food Products: Application to Wheat Crackers
Batista, A. P., Niccolai, A., Bursic, I., Sousa, I., Raymundo, A., Rodolfi, L., Biondi, N., & Tredici, M. R. (2019) · Foods · doi:10.3390/foods8120611 · Cited by 203 · see citing works
Carotenoid Production from Microalgae: Biosynthesis, Salinity Responses and Novel Biotechnologies
Ren, Y., Sun, H., Deng, J., Huang, J., & Chen, F. (2021) · Marine Drugs · doi:10.3390/md19120713 · Cited by 193 · see citing works
Recent updates on bioaccessibility of phytonutrients
Thakur, N., Raigond, P., Singh, Y., Mishra, T., Singh, B. K., Lal, M. K., & Dutt, S. (2020) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2020.01.019 · Cited by 187 · see citing works
Digestibility, bioaccessibility and bioactivity of compounds from algae
Demarco, M., de Moraes, J. O., Matos, Â. P., Dérner, R. B., Neves, F. D. F., & Tribuzi, G. (2022) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2022.02.004 · Cited by 181 · see citing works
Lafarga, T., Acién, F. G., Castellari, M., Villaró, S., Bobo, G., & Aguiló‐Aguayo, I. (2019) · LWT · doi:10.1016/j.lwt.2019.05.037 · Cited by 131 · see citing works
Self-citationCharacterization and Bioavailability of Vitamin B12-Compounds from Edible Algae.
Watanabe, F., Takenaka, S., Kittaka‐Katsura, H., Ebara, S., & Miyamoto, E. (2002) · Journal of Nutritional Science and Vitaminology · doi:10.3177/jnsv.48.325 · Cited by 128 · see citing works
Rao, A. R., Baskaran, V., Sarada, R., & Ravishankar, G. A. (2013) · Food Research International · doi:10.1016/j.foodres.2013.07.067 · Cited by 127 · see citing works
Custódio, L. M. B., Justo, T., Silvestre, L., Barradas, A. M., Vizetto‐Duarte, C., Pereira, H., Barreira, L. A., Rauter, A. P., Alberício, F., & Varela, J. C. S. (2011) · Food Chemistry · doi:10.1016/j.foodchem.2011.08.047 · Cited by 125 · see citing works
Niccolai, A., Venturi, M., Galli, V., Pini, N., Rodolfi, L., Biondi, N., D’Ottavio, M., Batista, A. P., Raymundo, A., Granchi, L., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-55840-1 · Cited by 124 · see citing works
Gohara-Beirigo, A. K., Matsudo, M. C., Cezare-Gomes, E. A., de Carvalho, J. C. M., & Danesi, E. D. G. (2022) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2022.04.030 · Cited by 97 · see citing works
Recommended Dietary Allowances, 10th Edition
(1989) · Journal of the American Dietetic Association · doi:10.1016/s0002-8223(21)22412-7 · Cited by 97 · see citing works
Millar, K. A., Barry‐Ryan, C., Burke, R. M., Hussey, K., McCarthy, S. N., & Gallagher, E. (2017) · International Journal of Food Science & Technology · doi:10.1111/ijfs.13388 · Cited by 74 · see citing works
Pão Sem Glúten Enriquecido Com a Microalga Spirulina platensis
Silva FIGUEIRA, F. D., Moraes CRIZEL, T. D., Rubira SILVA, C., & las Mercedes SALAS-MELLADO, M. D. (2011) · Brazilian Journal of Food Technology · doi:10.4260/bjft2011140400037 · Cited by 71 · see citing works
Free amino acid profiling in the giant puffball mushroom (Calvatia gigantea) using UPLC–MS/MS
Kıvrak, İ., Kıvrak, Ş., & Harmandar, M. (2014) · Food Chemistry · doi:10.1016/j.foodchem.2014.02.108 · Cited by 71 · see citing works
Calella, P., Cerullo, G., Di Dio, M., Liguori, F., Di Onofrio, V., Gallè, F., & Liguori, G. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2022.1048258 · Cited by 69 · see citing works
Verni, M., Demarinis, C., Rizzello, C. G., & Pontonio, E. (2023) · Foods · doi:10.3390/foods12050983 · Cited by 69 · see citing works
Bioaccessibility and antioxidant activity of phenolic compounds in cooked pulses
Lafarga, T., Villaró, S., Bobo, G., Simó, J., & Aguiló‐Aguayo, I. (2019) · International Journal of Food Science & Technology · doi:10.1111/ijfs.14082 · Cited by 65 · see citing works
Self-citationLafarga, T., Mayre, E., Echeverría, G., Viñas, İ., Villaró, S., Acién, F. G., Castellari, M., & Aguiló‐Aguayo, I. (2019) · LWT · doi:10.1016/j.lwt.2019.108439 · Cited by 65 · see citing works
Self-citationTerriente-Palacios, C., & Castellari, M. (2021) · Food Chemistry · doi:10.1016/j.foodchem.2021.130770 · Cited by 62 · see citing works
Hernández-López, I., Benavente‐Valdés, J. R., Castellari, M., Aguiló‐Aguayo, I., Morillas‐España, A., Sánchez‐Zurano, A., Acién, F. G., & Lafarga, T. (2021) · Algal Research · doi:10.1016/j.algal.2021.102361 · Cited by 51 · see citing works
Self-citationMontevecchi, G., Santunione, G., Licciardello, F., Köker, Ö., Masino, F., & Antonelli, A. (2022) · Food Research International · doi:10.1016/j.foodres.2022.111357 · Cited by 51 · see citing works
Effect of Spirulina in Bread Formulated with Wheat Flours of Different Alveograph Strength
Hernández-López, I., Alamprese, C., Cappa, C., Prieto-Santiago, V., Abadías, M., & Aguiló‐Aguayo, I. (2023) · Foods · doi:10.3390/foods12203724 · Cited by 49 · see citing works
Self-citationConsumer perception and acceptability of microalgae based breadstick
García‐Segovia, P., Alcaraz, V. G., Tárrega, A., & Martı́nez-Monzó, J. (2020) · Food Science and Technology International · doi:10.1177/1082013220906235 · Cited by 39 · see citing works
Spatiotemporal trends in adequacy of dietary nutrient production and food sources
Geyik, Ö., Hadjikakou, M., & Bryan, B. A. (2020) · Global Food Security · doi:10.1016/j.gfs.2020.100355 · Cited by 35 · see citing works
Recommended dietary allowances, tenth edition.
Palmer, S. (1990) · PubMed · Cited by 24 · see citing works
Evaluation of the prospects for the use of microalgae in functional bread production
Sukhikh, S., Ivanova, S., Dolganyuk, V., Pilevinova, I., Prosekov, A., Ulrikh, E., Noskova, S., Michaud, P., & Babich, O. (2022) · Applied Sciences · doi:10.3390/app122412563 · Cited by 22 · see citing works
Muszyńska, B., Krakowska, A., Lazur, J., Jękot, B., Zimmer, Ł., Szewczyk, A., Sułkowska‐Ziaja, K., Poleszak, E., & Opoka, W. (2017) · Journal of Applied Phycology · doi:10.1007/s10811-017-1357-2 · Cited by 19 · see citing works
Moon, S.-H., & Cho, S. (2023) · Algal Research · doi:10.1016/j.algal.2023.103328 · Cited by 16 · see citing works
Nicolau‐Lapeña, I., Abadías, M., Bobo, G., Lafarga, T., Viñas, İ., & Aguiló‐Aguayo, I. (2021) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.16041 · Cited by 8 · see citing works
Self-citationApplication of microalgae in baked goods and pasta
Raymundo, A., Fradinho, P., & Nunes, M. C. (2023) · Elsevier eBooks · doi:10.1016/b978-0-323-99196-4.00022-x · Cited by 3 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Martínez-Ruiz, F., Andrade-Bustamante, G., Holguín-Peña, R., Renganathan, P., Gaysina, L., Sukhanova, N., & Puente, E. (2025) · Biomass · doi:10.3390/biomass5020025 · Cited by 51 · see citing works
Girotto, F., & Scapini, A. (2025) · Algal Research · doi:10.1016/j.algal.2025.104288 · Cited by 29 · see citing works
Šola, I., Poljuha, D., Pavičić, I., Tušek, A. J., & Šamec, D. (2025) · Foods · doi:10.3390/foods14030416 · Cited by 24 · see citing works
Sivaramakrishnan, R., Kanwal, S., Incharoensakdi, A., Nirmal, N. P., & Srimongkol, P. (2025) · Journal of Agriculture and Food Research · doi:10.1016/j.jafr.2025.102148 · Cited by 17 · see citing works
Marine Algae‐Derived Bioactives: A Sustainable Resource for the Food and Agriculture Industries
Chadorshabi, S., Mohammadi, M., Shamloo, E., & Mahmoudzadeh, M. (2025) · Food Frontiers · doi:10.1002/fft2.70082 · Cited by 14 · see citing works
Nikolić, I., Lončarević, I., Rakita, S., Čabarkapa, I., Vulić, J., Takači, A., & Petrović, J. (2025) · Processes · doi:10.3390/pr13030908 · Cited by 10 · see citing works
Su, K., Ma, J., Li, Q., ZHANG, X., & Cheng, L. (2026) · Foods · doi:10.3390/foods15050811 · Cited by 3 · see citing works
Zhao, N., Chu, Y., Huang, Q., Qin, S., Cui, H., & Zhang, W. (2026) · Algal Research · doi:10.1016/j.algal.2026.104629 · Cited by 2 · see citing works
Advancing 3D-Printed Seafood Analogues with Tetraselmis chui and Tetraselmis chui Water Extract
Moreira, C., Molina-Montero, C., Ferreira‐Santos, P., Teixeira, J. A., Rocha, C. M. R., & Martı́nez-Monzó, J. (2025) · Food and Bioprocess Technology · doi:10.1007/s11947-025-03965-9 · Cited by 1 · see citing works
Bio-Based Bakery Products: A Present Insight on Its Nutritive Potential
Nefertari, C.-G. S., Cruz‐Casas, D. E., Campos‐Múzquiz, L. G., Carolina, F.-G. A., Javier, P.-P. A., Rangel-Ortega, S. D. C., García-Ortíz, J. D., Orozco-Sifuentes, M. M., Soriano‐Melgar, L. D. A. A., & Rodrı́guez-Herrera, R. (2024) · Springer eBooks · doi:10.1007/978-981-97-8561-2_1 · Cited by 2 · see citing works
Microalgae as Sustainable and Economic Sources of Omega-3 Fatty Acids
Panchal, S. K., Heimann, K., & Brown, L. (2025) · Springer eBooks · doi:10.1007/978-3-031-84200-9_6 · Cited by 1 · see citing works
Boza, A., Lemos, E., Bürck, M., Gautério, G. V., & Braga, A. R. C. (2026) · Macromol · doi:10.3390/macromol6030054
Zupo, R., Castellana, F., Crupi, P., Annunziato, A., Corbo, F., & Clodoveo, M. L. (2026) · Journal of Food Process Engineering · doi:10.1111/jfpe.70310
Dutta, S., Hazarika, S., & Sardar, S. (2026) · Springer eBooks · doi:10.1007/978-3-032-22521-4_17 · Cited by 1 · see citing works
Хлорелла как фактор повышения пищевой ценности хлебобулочных изделий
Тарасова, В. В., & Кувшинов, И. Е. (2026) · Food processing industry · doi:10.52653/ppi.2026.9.9.027
Álvarez‐Arteaga, P., Lazo‐Vélez, M. A., Cordero‐Clavijo, L. M., & Paterson, S. (2026) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.70687
- Authors
- Hernández-López, I., Abadias, M., Prieto-Santiago, V., Chic-Blanco, Á., Ortiz-Solà, J., & Aguiló-Aguayo, I.
- Affiliations
- Institute of Agrifood Research and Technology (ES)
- IRTA, Postharvest Programme, Parc Agrobiotech Lleida. Parc de Gardeny, Edifici Fruitcentre, 25003 Lleida, Spain
- Venue
- Foods
- Publisher
- MDPI AG
- Published
- 2023-12-26
- Volume
- 13
- Issue
- 1
- Pages
- 84
- ISSN
- 2304-8158 (ISSN-L)
- PMC
- PMC10778747
- OpenAlex
- W4390236743
- Cited by
- 16
- References
- 41
- Citations by year
- 2026: 7 · 2025: 8 · 2024: 1
- FWCI
- 3.28
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Foods (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- IRTA (Institut de Recerca i Tecnologia Agroalimentaries) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- ProFuture project (862980, RYC-2016-19949)
- European Union’s Horizon 2020 research and innovation programm (862980, RYC-2016-19949)
- CERCA Programme (862980, RYC-2016-19949)
- National Programme for the Promotion of Talent and Its Employability of the ‘Ministerio de Economía, Industria y Competitividad’ of the Spanish Government (862980, RYC-2016-19949)
- European Social Fund (862980, RYC-2016-19949)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
- Life Sciences › Agricultural and Biological Sciences › Food Science › Seed and Plant Biochemistry
- Keywords
- microalgae, Spirulina, Chlorella, Tetraselmis, bakery products, crackers, functional food ingredients, antioxidant capacity, total phenolic content, amino acid composition, sensory acceptability, flour substitution, nutritional value
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review