Effects of Ambient Fine Particles PM2.5 on Human HaCaT Cells
Open accessAbstract
The current study was conducted to observe the effects of fine particulate matter (PM2.5) on human keratinocyte cell line (HaCaT) cells. The potential mechanism linking PM2.5 and skin was explored. HaCaT cells were cultured and then accessed in plate with PM2.5. Cell viability was tested by Cell Counting Kit-8. The mRNA and protein expression of Filaggrin, Loricrin, Involucrin, and Repetin were analyzed. The levels of Granulocyte-macrophage Colony Stimulating Factor, Thymic Stromal Lymphopoietin, Tumor Necrosis Factor-α, Interleukin-1α, and Interleukin-8 were detected in the supernatant of the HaCaT cell with enzyme-linked immunosorbent assay kits. Cell viability decreased with the increase in PM2.5. Compared with the control group, the protein expression of Filaggrin, Repetin, Involucrin, and Loricrin showed different expression patterns in PM2.5 treatment groups. The level of Tumor Necrosis Factor-α, Thymic Stromal Lymphopoietin, Interleukin-1α, and Interleukin-8 significantly increased in the cells treated with PM2.5. Ambient PM2.5 may increase the risk of eczema and other skin diseases. The relative mechanism may be associated with the impairment of the skin barrier and the elevation of inflammatory responses.
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These PM2.5 doses (used in earlier studies) were cytotoxic to primary keratinocytes in the current study.
“We found that these PM2.5 doses were cytotoxic to primary keratinocytes in the current study (27–29).”
From Particulate matter causes skin barrier dysfunction. (2021) · cites this paper as [28]
The Methods (§2.3) show that this earlier study used a range of PM2.5 doses, and the Results (§3.2) report reduced cell viability at higher doses. However, it tested immortalized HaCaT keratinocytes, not primary keratinocytes; the finding in the citing authors’ current study is not a finding of this source. Page numbers are unavailable in our copy. (LLM, conf=0.87)
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- [1]Ministry of Environmental Protection of the P.R. China General Administration of Quality Supervision, Inspection and Quarantine of the P.R. China Ambient Air Quality Standards GB 3095-2012 Standard Press of China Beijing, China 2012
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BackgroundAir pollution and skin diseases: A comprehensive evaluation of the associated mechanism.
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- Authors
- Li, Q., Kang, Z., Jiang, S., Zhao, J., Yan, S., Xu, F., & Xu, J.
- Affiliations
- Shanghai Medical College of Fudan University (CN)
- Fudan University (CN)
- Corresponding author
- Xu, Feng
- Xu, Jinhua
- Venue
- International Journal of Environmental Research and Public Health
- Publisher
- MDPI AG
- Published
- 2017-01-12
- Volume
- 14
- Issue
- 1
- Pages
- 72
- ISSN
- 1660-4601 (ISSN-L)
- PMC
- PMC5295323
- OpenAlex
- W2572908310
- Cited by
- 77
- References
- 30
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 6 · 2023: 6 · 2022: 10 · 2021: 15 · 2020: 13 · 2019: 9 · 2018: 3 · 2017: 5
- FWCI
- 4.42
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Environmental Research and Public Health (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- National Natural Science Foundation of China (81301361)
- Shanghai Key Laboratory of Meteorology and Health
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
- Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
- Health Sciences › Medicine › Dermatology › Contact Dermatitis and Allergies
- Keywords
- PM2.5, HaCaT keratinocytes, skin barrier, filaggrin, involucrin, loricrin, cytokines, thymic stromal lymphopoietin, TNF-α, interleukin-8, interleukin-1 alpha, cell viability, atopic dermatitis
- MeSH terms
- Filaggrin Proteins, Thymic Stromal Lymphopoietin, Cell Line, Cell Survival, Cells, Cultured, Enzyme-Linked Immunosorbent Assay, Humans, Protein Precursors, Skin, Tumor Necrosis Factor-alpha, Keratinocytes, Granulocyte-Macrophage Colony-Stimulating Factor, Cytokines, Interleukin-8, Particulate Matter, Interleukin-1alpha
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