UPLC-MS/MS-based profiling of eicosanoids in RAW264.7 cells treated with lipopolysaccharide
Open accessAbstract
While both the pro- and anti-inflammatory effects of several eicosanoids have been widely studied, the degree of inflammation in cells that results from various eicosanoids has yet to be comprehensively studied. The objective of this study was to assess the effect of lipopolysaccharide (LPS) treatment on eicosanoid content in RAW264.7 cells. An Ultra performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS)-based profiling method was used to analyze the eicosanoid contents of RAW264.7 cells treated with different LPS concentrations. The profiling data were subjected to statistical analyses, such as principal component analysis (PCA) and hierarchical clustering analysis. LPS treatment increased nitric oxide production and secretion of pro-inflammatory cytokines, such as tumor necrosis factor-α and interleukin-6, in a concentration-dependent manner. In total, 79 eicosanoids were identified in the cells. RAW264.7 cells treated with different LPS concentrations were well differentiated in the PCA score plot. A heatmap was used to identify the eicosanoids that were up- or down-regulated according to the degree of inflammation and LPS concentration. Thirty-nine eicosanoids were upregulated and seven were down-regulated by LPS treatment in a concentration-dependent manner. Our novel UPLC-MS/MS technique can profile eicosanoids, and can evaluate the correlations between inflammation and eicosanoid metabolism.
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Dihomo-15d-PGJ2 is highly elevated and abundant at about 1-5 μM in senescent cells.
“Dihomo-15d-PGJ2 was highly elevated and abundant (~1–5 μM) in senescent cells - but is considerably unstudied (detected in one published list of analytes) (Lee et al., 2016).”
From Oxylipin biosynthesis reinforces cellular senescence and allows detection of senolysis (2021) · cites this paper as Lee et al., 2016
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Oxylipin biosynthesis reinforces cellular senescence and allows detection of senolysis
Wiley, C. D., Sharma, R., Davis, S. S., Lopez-Dominguez, J. A., Mitchell, K. P., Wiley, S., Alimirah, F., Kim, D. E., Payne, T., Rosko, A., et al. (2021) · Cell Metabolism · doi:10.1016/j.cmet.2021.03.008 · Cited by 173 · see citing works
- Authors
- Lee, J., Mok, H., Lee, D.-Y., Park, S., Ban, M., Choi, J., Park, C., Ahn, Y.-S., Kim, K., & Kim, H.
- Affiliations
- Rural Development Administration (KR)
- Kyung Hee University (KR)
- Chungbuk National University (KR)
- Corresponding author
- Kim, Kwang
- Kim, Hyung
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2016-04-06
- Volume
- 17
- Issue
- 4
- Pages
- 508
- ISSN
- 1422-0067 (ISSN-L)
- PMC
- PMC4848964
- OpenAlex
- W2314682872
- Cited by
- 22
- References
- 42
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 3 · 2021: 3 · 2020: 6 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 1
- FWCI
- 1.89
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Molecular Sciences (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Rural Development Administration (PJ01135001)
- Topics
- Health Sciences › Medicine › Pharmacology › Inflammatory mediators and NSAID effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Eicosanoids and Hypertension Pharmacology
- Keywords
- eicosanoids, lipopolysaccharide, RAW 264.7 macrophages, inflammation, nitric oxide, TNF-α, interleukin-6, eicosanoid metabolism, concentration-dependent effects
- MeSH terms
- RAW 264.7 Cells, Animals, Chromatography, High Pressure Liquid, Inflammation, Lipopolysaccharides, Macrophages, Nitric Oxide, Tumor Necrosis Factor-alpha, Eicosanoids, Interleukin-6, Mice, Tandem Mass Spectrometry
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