The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Applications of CRISPR/Cas System to Bacterial Metabolic Engineering
Open accessAbstract
The clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas) adaptive immune system has been extensively used for gene editing, including gene deletion, insertion, and replacement in bacterial and eukaryotic cells owing to its simple, rapid, and efficient activities in unprecedented resolution. Furthermore, the CRISPR interference (CRISPRi) system including deactivated Cas9 (dCas9) with inactivated endonuclease activity has been further investigated for regulation of the target gene transiently or constitutively, avoiding cell death by disruption of genome. This review discusses the applications of CRISPR/Cas for genome editing in various bacterial systems and their applications. In particular, CRISPR technology has been used for the production of metabolites of high industrial significance, including biochemical, biofuel, and pharmaceutical products/precursors in bacteria. Here, we focus on methods to increase the productivity and yield/titer scan by controlling metabolic flux through individual or combinatorial use of CRISPR/Cas and CRISPRi systems with introduction of synthetic pathway in industrially common bacteria including Escherichia coli. Further, we discuss additional useful applications of the CRISPR/Cas system, including its use in functional genomics.
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Citations
Cites
The new frontier of genome engineering with CRISPR-Cas9
Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
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“Thus, the CRISPRi system can easily regulate gene expression levels by directing sgRNA to the region of non-template DNA of target genes [8].”
BackgroundDNA targeting specificity of RNA-guided Cas9 nucleases
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CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
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Bioactive Microbial Metabolites: A Personal View
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Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Konermann, S., Brigham, M. D., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., Nishimasu, H., et al. (2015) · Nature · doi:10.1038/nature14136 · Cited by 2,825
Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
Gilbert, L. A., Horlbeck, M. A., Adamson, B., Villalta, J. E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C., et al. (2014) · Cell · doi:10.1016/j.cell.2014.09.029 · Cited by 2,728
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
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CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo
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Editing DNA Methylation in the Mammalian Genome
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“This system has been intensively repurposed for genome engineering, metabolic engineering [20], epigenetic modification [21], functional genomics [11], genome imaging [22], and DNA/RNA chromatin immunoprecipitation [23].”
Method
Cited by
CRISPR/Cas system: recent advances and future prospects for genome editing
Manghwar, H., Lindsey, K., Zhang, X., & Jin, S. (2019) · Trends in Plant Science · doi:10.1016/j.tplants.2019.09.006 · Cited by 529
“In bacterial cells, CRISPRi is a more useful genetic engineering tool for gene knockdown compared with RNAi, because it avoids cell death through disruption of the genome [85].”
BackgroundMicrobial Interventions in Bioremediation of Heavy Metal Contaminants in Agroecosystem
Pande, V., Pandey, S. C., Sati, D., Bhatt, P., & Samant, M. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.824084 · Cited by 373
Microbial Pigments in the Food Industry—Challenges and the Way Forward
Sen, T., Barrow, C. J., & Deshmukh, S. K. (2019) · Frontiers in Nutrition · doi:10.3389/fnut.2019.00007 · Cited by 367
Pattharaprachayakul, N., Lee, M., Incharoensakdi, A., & Woo, H. M. (2020) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2020.109619 · Cited by 43
Christi, K., Hudson, J., & Egan, S. (2024) · Microbiological Research · doi:10.1016/j.micres.2024.127729 · Cited by 8
Metadata
- Authors
- Cho, S., Shin, J., & Cho, B.-K.
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2018-04-05
- Volume
- 19
- Issue
- 4
- Pages
- 1089
- ISSN
- 1422-0067 (ISSN-L)
- OpenAlex
- W2795717814
- Cited by
- 169
- References
- 12
- FWCI
- 5.41
- Type
- journal article
- Language
- en
- Open access
- gold
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