Synthetic Retinoid Seletinoid G Improves Skin Barrier Function through Wound Healing and Collagen Realignment in Human Skin Equivalents
Open accessAbstract
The outer epidermal skin is a primary barrier that protects the body from extrinsic factors, such as ultraviolet (UV) radiation, chemicals and pollutants. The complete epithelialization of a wound by keratinocytes is essential for restoring the barrier function of the skin. However, age-related alterations predispose the elderly to impaired wound healing. Therefore, wound-healing efficacy could be also considered as a potent function of an anti-aging reagent. Here, we examine the epidermal wound-healing efficacy of the fourth-generation retinoid, seletinoid G, using HaCaT keratinocytes and skin tissues. We found that seletinoid G promoted the proliferation and migration of keratinocytes in scratch assays and time-lapse imaging. It also increased the gene expression levels of several keratinocyte proliferation-regulating factors. In human skin equivalents, seletinoid G accelerated epidermal wound closure, as assessed using optical coherence tomography (OCT) imaging. Moreover, second harmonic generation (SHG) imaging revealed that seletinoid G recovered the reduced dermal collagen deposition seen in ultraviolet B (UVB)-irradiated human skin equivalents. Taken together, these results indicate that seletinoid G protects the skin barrier by accelerating wound healing in the epidermis and by repairing collagen deficiency in the dermis. Thus, seletinoid G could be a potent anti-aging agent for protecting the skin barrier.
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HSEs are used to investigate wound healing, aging, and various diseases.
“HSEs are used to investigate various aspects of normal and abnormal skin biology, including wound healing [40,41], aging [42,43], and the study of various diseases [18].”
From Diversity of human skin three-dimensional organotypic cultures (2024) · cites this paper as [41]
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- Authors
- Lee, E.-S., Ahn, Y., Bae, I.-H., Min, D., Park, N. H., Jung, W., Kim, S.-H., Hong, Y. D., Park, W. S., & Lee, C. S.
- Affiliations
- Amorepacific (South Korea) (KR)
- Ulsan National Institute of Science and Technology (KR)
- Korea Research Institute of Standards and Science (KR)
- Eulji University (KR)
- Corresponding author
- Lee, Chang Seok
- ORCID
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2020-04-30
- Volume
- 21
- Issue
- 9
- Pages
- 3198
- ISSN
- 1422-0067 (ISSN-L)
- PMC
- PMC7247558
- OpenAlex
- W3022479612
- Cited by
- 25
- References
- 31
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 4 · 2023: 3 · 2022: 2 · 2021: 7 · 2020: 3 · 2014: 1
- FWCI
- 2.74
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- International Journal of Molecular Sciences (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Scholarworks@UNIST (Ulsan National Institute of Science and Technology) (submittedVersion, cc-by)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Keywords
- synthetic retinoids, skin barrier function, wound healing, keratinocyte proliferation, keratinocyte migration, collagen deposition, UVB-induced skin damage, anti-aging agents, human skin equivalents, HaCaT keratinocytes, optical coherence tomography, second harmonic generation microscopy
- MeSH terms
- Cell Line, Cell Movement, Cell Survival, Dioxolanes, Epidermis, Humans, Pyrans, Skin, Ultraviolet Rays, Wound Healing, Keratinocytes, Tomography, Optical Coherence, Cell Proliferation
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