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Cited by 91Pluripotent Stem Cells ResearchTissue Engineering and Regenerative MedicineCardiac electrophysiology and arrhythmias

Modeling Cardiovascular Diseases with hiPSC-Derived Cardiomyocytes in 2D and 3D Cultures

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Sacchetto, Claudia, Vitiello, Libero, de Windt, Leon J., Rampazzo, Alessandra, Calore, Martina·2020·International Journal of Molecular Sciences
DOI 10.3390/ijms21093404PMC PMC7246991
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Abstract

In the last decade, the generation of cardiac disease models based on human-induced pluripotent stem cells (hiPSCs) has become of common use, providing new opportunities to overcome the lack of appropriate cardiac models. Although much progress has been made toward the generation of hiPSC-derived cardiomyocytes (hiPS-CMs), several lines of evidence indicate that two-dimensional (2D) cell culturing presents significant limitations, including hiPS-CMs immaturity and the absence of interaction between different cell types and the extracellular matrix. More recently, new advances in bioengineering and co-culture systems have allowed the generation of three-dimensional (3D) constructs based on hiPSC-derived cells. Within these systems, biochemical and physical stimuli influence the maturation of hiPS-CMs, which can show structural and functional properties more similar to those present in adult cardiomyocytes. In this review, we describe the latest advances in 2D- and 3D-hiPSC technology for cardiac disease mechanisms investigation, drug development, and therapeutic studies.

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  • EHTs demonstrate good predictive value for human (patho-)physiology.

    “EHTs demonstrate good predictive value for human (patho-)physiology (Sacchetto et al., 2020) and have been successfully explored for disease modelling and drug compound screening (Hansen et al., 2010;Nunes et al., 2013;Schwach et al., 2024).”

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Cited by

Sorted by citations

Authors
Sacchetto, C., Vitiello, L., de Windt, L. J., Rampazzo, A., & Calore, M.
Affiliations
  • University of Padua (IT)
  • Department of Molecular Genetics, University of Maastricht, Universiteitssingel 50, 6229ER Maastricht, The Netherlands
  • Maastricht University (NL)
Corresponding author
  • Rampazzo, Alessandra
  • Calore, Martina
ORCID
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2020-05-11
Volume
21
Issue
9
Pages
3404
ISSN
1422-0067 (ISSN-L)
DOI
10.3390/ijms21093404
PMC
PMC7246991
OpenAlex
W3023798838
Cited by
91
References
13
Citations by year
2026: 14 · 2025: 11 · 2024: 18 · 2023: 14 · 2022: 11 · 2021: 18 · 2020: 2 · 2019: 3
FWCI
1.10
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Research Publications (Maastricht University) (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Padua Research Archive (University of Padova) (submittedVersion, cc-by)
  • Research Publications (Maastricht University) (submittedVersion, cc-by)
Funders
European Research Council (311549)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
Keywords
hiPSC-derived cardiomyocytes, cardiovascular disease models, 2D cell culture limitations, cardiomyocyte maturation, co-culture model, extracellular matrix, bioengineering, drug development, therapeutic studies
MeSH terms
Cardiovascular Diseases, Cell Differentiation, Cells, Cultured, Humans, Models, Cardiovascular, Cell Culture Techniques, Tissue Engineering, Myocytes, Cardiac, Tissue Scaffolds, Induced Pluripotent Stem Cells

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