Biotechnological advances and applications of human pluripotent stem cell-derived heart models
Open accessAbstract
In recent years, significant biotechnological advancements have been made in engineering human cardiac tissues and organ-like models. This field of research is crucial for both basic and translational research due to cardiovascular disease being the leading cause of death in the developed world. Additionally, drug-associated cardiotoxicity poses a major challenge for drug development in the pharmaceutical and biotechnological industries. Progress in three-dimensional cell culture and microfluidic devices has enabled the generation of human cardiac models that faithfully recapitulate key aspects of human physiology. In this review, we will discuss 3D pluripotent stem cell (PSC)-models of the human heart, such as engineered heart tissues and organoids, and their applications in disease modeling and drug screening.
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There is a great need for more predictive human-based cardiac in vitro models to enhance CVD therapeutic strategies and avoid drug-induced cardiotoxicity.
“Thus, there is a great need for more predictive human-based cardiac in vitro models to enhance CVD therapeutic strategies and avoid drug-induced cardiotoxicity (Marshall et al., 2018; Muniyandi et al., 2023).”
From Mini-hearts for disease modeling and drug testing – process optimization versus biological functionality (2026) · cites this paper as Muniyandi et al., 2023
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Cited by
Sorted by citations
O’Hern, C., Caywood, S., Aminova, S., Kiselev, A. M., Volmert, B. D., Cao, W., Wang, F., Dionise, M., Sewavi, M.-L., Skoric, M., et al. (2025) · Cell stem cell · doi:10.1016/j.stem.2025.09.011 · Cited by 37 · see citing works
Self-citationProgress of organoid platform in cardiovascular research
Du, X., Jia, H., Chang, Y., Zhao, Y., & Song, J. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.05.043 · Cited by 21 · see citing works
O’Hern, C., Caywood, S., Aminova, S., Kiselev, A. M., Volmert, B. D., Wang, F., Sewavi, M.-L., Cao, W., Dionise, M., Muniyandi, P., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.11.13.623051 · Cited by 11 · see citing works
Self-citationA Concise Review of Organoid Tissue Engineering: Regenerative Applications and Precision Medicine
Makesh, K. Y., Navaneethan, A., Ajay, M., Munuswamy‐Ramanujam, G., Chinnasamy, A., Gnanasampanthapandian, D., & Palaniyandi, K. (2025) · Organoids · doi:10.3390/organoids4030016 · Cited by 12 · see citing works
Yang, Y., Yang, H., Kiskin, F. N., & Zhang, J. Z. (2024) · Medical Review · doi:10.1515/mr-2023-0059 · Cited by 9 · see citing works
Stem Cells to Organoids: Pioneering the Future of Regenerative Therapies
Kumar, D., Gupta, S., Gupta, V., Tanwar, R., Rani, P., & Bansal, V. (2025) · Stem Cell Reviews and Reports · doi:10.1007/s12015-025-10985-7 · Cited by 6 · see citing works
Advances in regenerative cardiology: Stem cells versus bioartificial tissues
Fuentes‐Mendoza, J. M., Moreno, J. M. M., Concepción‐Zavaleta, M. J., Urteaga, L. A. C., Paz‐Ibarra, J., Gonzales‐Valdivieso, R., Castillo‐Velásquez, F. G., Burgos‐Castillo, A. S., Garza‐Boullosa, R., & Núñez‐Pérez, V. D. (2025) · European Journal of Clinical Investigation · doi:10.1111/eci.70101 · Cited by 3 · see citing works
Kaushik, A., Mishra, D. T. P., Solanki, R., & Bhatia, D. D. (2026) · ACS Pharmacology & Translational Science · doi:10.1021/acsptsci.5c00630 · Cited by 2 · see citing works
Zou, X., Wang, F., Zheng, H., Liu, X., Kong, T., Jiang, R., Guo, Y., Liang, Y., Zhao, H., Wang, B., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-73822-6 · Cited by 2 · see citing works
Lee, S.-J., & Lee, H. (2024) · Organoid · doi:10.51335/organoid.2024.4.e6 · Cited by 1 · see citing works
Hecking, J., Cano-Jorge, M., Passier, R., & Rivera-Arbeláez, J. M. (2026) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2025.1719533
“Thus, there is a great need for more predictive human-based cardiac in vitro models to enhance CVD therapeutic strategies and avoid drug-induced cardiotoxicity (Marshall et al., 2018; Muniyandi et al., 2023).”
van Noort, D., & Mandenius, C. (2025) · Methods in molecular biology · doi:10.1007/978-1-0716-4530-7_12
Taghiyev, Z. T., Arneth, B., Altrawy, A., Mohamed, R., Ghazy, A., Hamdy, D., & Sabit, H. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-29890-5.00001-4
Yang, H., Zeng, Y., Teng, Y., Zhao, Q., Hou, M., Cheng, Z., & Wang, X. Y. (2026) · Stem Cell Research & Therapy · doi:10.1186/s13287-026-04996-5 · Cited by 1 · see citing works
- Authors
- Muniyandi, P., O’Hern, C., Popa, M. A., & Aguirre, A.
- Affiliations
- Michigan State University (US)
- Corresponding author
- Aguirre, Aitor
- Venue
- Frontiers in Bioengineering and Biotechnology
- Publisher
- Frontiers Media SA
- Published
- 2023-07-25
- Volume
- 11
- Pages
- 1214431-1214431
- ISSN
- 2296-4185 (ISSN-L)
- PMC
- PMC10407810
- OpenAlex
- W4385256660
- Cited by
- 14
- References
- 183
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 4
- FWCI
- 0.37
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Bioengineering and Biotechnology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- American Heart Association (19IPLOI34660342)
- National Institutes of Health
- National Heart, Lung, and Blood Institute (K01HL135464, R01HL151505)
- National Institute of Diabetes and Digestive and Kidney Diseases (P30DK020572)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- human pluripotent stem cells, cardiac tissue engineering, cardiac organoids, 3D cell culture, microfluidics, disease modeling, drug screening, cardiotoxicity, cardiovascular disease, cardiac models
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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