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Cited by 3Retinal Development and DisordersMicroRNA in disease regulationNeuroscience and Neuropharmacology Research

Profile of miR-23 Expression and Possible Role in Regulation of Glutamic Acid Decarboxylase during Postnatal Retinal Development

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Pöstyéni, Etelka, Kovács-Valasek, Andrea, Urbán, Péter, Czuni, Lilla, Sétáló, György, Fekete, Csaba, Gabriel, Robert·2021·International Journal of Molecular Sciences
DOI 10.3390/ijms22137078PMC PMC8268301
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Abstract

As neurotransmitter, GABA is fundamental for physiological processes in the developing retina. Its synthesis enzymes are present during retinal development, although the molecular regulatory mechanisms behind the changes in expression are not entirely understood. In this study, we revealed the expression patterns of glutamic acid decarboxylase 67(GAD67) and its coding gene (GAD1) and its potential miRNA-dependent regulation during the first three postnatal weeks in rat retina. To gain insight into the molecular mechanisms, miRNA-sequencing supported by RT-qPCR and in situ hybridization were carried out. GAD1 expression shows an increasing tendency, peaking at P15. From the in silico-predicted GAD1 targeting miRNAs, only miR-23 showed similar expression patterns, which is a known regulator of GAD1 expression. For further investigation, we made an in situ hybridization investigation where both GAD67 and miR-23 also showed lower expression before P7, with the intensity of expression gradually increasing until P21. Horizontal cells at P7, amacrine cells at P15 and P21, and some cells in the ganglion cell layer at several time points were double labelled with miR-23 and GAD67. Our results highlight the complexity of these regulatory networks and the possible role of miR-23 in the regulation of GABA synthesizing enzyme expression during postnatal retina development.

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Authors
Pöstyéni, E., Kovács-Valasek, A., Urbán, P., Czuni, L., Sétáló, G., Fekete, C., & Gabriel, R.
Affiliations
  • University of Pecs (HU)
  • János Szentágothai Research Centre, 7624 Pécs, Hungary
Corresponding author
  • Kovács-Valasek, Andrea
  • Gabriel, Robert
ORCID
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2021-06-30
Volume
22
Issue
13
Pages
7078
ISSN
1422-0067 (ISSN-L)
DOI
10.3390/ijms22137078
PMC
PMC8268301
OpenAlex
W3173515270
Cited by
3
References
1
Citations by year
2024: 1 · 2023: 2
FWCI
0.24
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • EFOP (3.-6.-2-16-2017-00008)
  • Hungarian Brain Research Program (2017-1.2.1-NKP-2017-00002)
  • NKFIH (119289)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
miR-23, glutamic acid decarboxylase 67, GAD1, GABA synthesis, postnatal retinal development, microRNA regulation, horizontal cells, amacrine cells, retinal ganglion cells, gene expression patterns, rat retina
MeSH terms
Animals, Glutamate Decarboxylase, Retina, Gene Expression Regulation, Enzymologic, Rats, Wistar, MicroRNAs, Rats

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