Cited by 33Ruminant Nutrition and Digestive PhysiologyAnimal Nutrition and PhysiologyGut microbiota and health

Effects of chestnut tannin extract, vescalagin and gallic acid on the dimethyl acetals profile and microbial community composition in rumen liquor: an in vitro study

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Mannelli, Federica, Daghio, Matteo, Alves, Susana P., Bessa, Rui J. B., Minieri, Sara, Giovannetti, Luciana, Conte, Giuseppe, Mele, Marcello +4 more·2019·Microorganisms

Abstract

The addition of polyphenol extracts in ruminant diets is an effective strategy to modulate rumen microflora. The aim of this in vitro trial was to study the effects of chestnut tannin extract (CHT), vescalagin (VES) and gallic acid (GAL) on dietary fibre degradability and on the dimethyl acetals (DMA) profile and microbial community composition of rumen liquor. Four diets (basal diet; basal diet plus CHT; basal diet plus VES; basal diet plus GAL) were fermented for 24 h using ewe rumen liquor. At the end of the fermentation, the microbial communities were characterized by sequencing the 16S rRNA gene. The DMA profile was analyzed by gas chromatography. Chestnut tannin extract did not affect fibre degradability, whereas VES and GAL showed a detrimental effect. The presence of CHT, VES and GAL influenced the concentration of several DMA (i.e., 12:0, 13:0, 14:0, 15:0, 18:0 and 18:1 trans-11), whereas the composition of the microbial community was marginally affected. The inclusion of CHT led to the enrichment of the genera Anaerovibrio, Bibersteinia, Escherichia/Shigella, Pseudobutyrivibrio and Streptococcus. The results of this study support the hypothesis that the activity of CHT is due to the synergistic effect of all components rather than the property of a single component.

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Authors
Mannelli, F., Daghio, M., Alves, S. P., Bessa, R. J. B., Minieri, S., Giovannetti, L., Conte, G., Mele, M., Messini, A., Rapaccini, S., Viti, C., & Buccioni, A.
Affiliations
  • University of Florence (IT)
  • University of Lisbon (PT)
  • University of Pisa (IT)
Corresponding author
Buccioni, Arianna
Venue
Microorganisms
Publisher
MDPI AG
Published
2019-07-18
Volume
7
Issue
7
Pages
202
ISSN
2076-2607 (ISSN-L)
OpenAlex
W2958800185
Cited by
33
References
48
Citations by year
2026: 6 · 2025: 3 · 2024: 6 · 2023: 4 · 2022: 5 · 2021: 5 · 2020: 4
FWCI
4.92
Type
journal article
Language
en
Open access
Locations
  • Microorganisms (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Florence Research (University of Florence) (submittedVersion, other-oa)
  • CINECA IRIS Institutial research information system (University of Pisa) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
Funders
Università degli Studi di Firenze (Characterization of rumen microbiota during the inclusion of different polyphenolic fractions)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
vescalagin, gallic acid, polyphenols, rumen microbiota, rumen fermentation, dimethyl acetals, rumen volatile fatty acids, rumen fluid, ewes, anaerobic bacteria

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