Oleacein Prevents High Fat Diet-Induced Adiposity and Ameliorates Some Biochemical Parameters of Insulin Sensitivity in Mice
Open accessAbstract
Oleacein is one of the most abundant polyphenolic compounds of olive oil, which has been shown to play a protective role against several metabolic abnormalities, including dyslipidemia, insulin resistance, and glucose intolerance. Herein, we investigated the effects of oleacein on certain markers of adipogenesis and insulin-resistance in vitro, in 3T3-L1 adipocytes, and in vivo in high-fat diet (HFD)-fed mice. During the differentiation process of 3T3-L1 preadipocytes into adipocytes, oleacein strongly inhibited lipid accumulation, and decreased protein levels of peroxisome proliferator-activated receptor gamma (PPARγ) and fatty acid synthase (FAS), while increasing Adiponectin levels. In vivo, treatment with oleacein of C57BL/6JOlaHsd mice fed with HFD for 5 and 13 weeks prevented the increase in adipocyte size and reduced the inflammatory infiltration of macrophages and lymphocytes in adipose tissue. These effects were accompanied by changes in the expression of adipose tissue-specific regulatory elements such as PPARγ, FAS, sterol regulatory element-binding transcription factor-1 (SREBP-1), and Adiponectin, while the expression of insulin-sensitive muscle/fat glucose transporter Glut-4 was restored in HFD-fed mice treated with oleacein. Collectively, our findings indicate that protection against HFD-induced adiposity by oleacein in mice is mediated by the modulation of regulators of adipogenesis. Protection against HFD-induced obesity is effective in improving peripheral insulin sensitivity.
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SREBP-1 and its target FAS protein levels were significantly reduced following oleacein treatment in liver and fat.
“Some of the molecular constituents, that appear to be involved in oleacein action, include some known nutrient-responsive regulators of lipid metabolism: the transcriptional activator sterol regulatory element-binding transcription factor-1 (SREBP-1), and its target fatty acid synthase (FAS), whose protein levels were significantly reduced following oleacein treatment in liver and fat [85,87].”
From Mediterranean diet nutrients to turn the tide against insulin resistance and related diseases (2020) · cites this paper as [87]
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- Authors
- Lepore, S. M., Maggisano, V., Bulotta, S., Mignogna, C., Arcidiacono, B., Procopio, A., Brunetti, A., Russo, D., & Celano, M.
- Affiliations
- Magna Graecia University (IT)
- Corresponding author
- Russo, Diego
- ORCID
- Venue
- Nutrients
- Publisher
- MDPI AG
- Published
- 2019-08-07
- Volume
- 11
- Issue
- 8
- Pages
- 1829
- ISSN
- 2072-6643 (ISSN-L)
- PMC
- PMC6723526
- OpenAlex
- W2965404289
- Cited by
- 34
- References
- 34
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 4 · 2023: 7 · 2022: 3 · 2021: 4 · 2020: 7 · 2019: 1 · 2013: 1
- FWCI
- 2.85
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- Nutrients (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- oleacein, olive oil polyphenols, high-fat diet, obesity, adipogenesis, insulin resistance, insulin sensitivity, 3T3-L1 adipocytes, PPARγ, fatty acid synthase, adiponectin, SREBP1, GLUT4, adipose tissue inflammation, macrophage infiltration, lipid accumulation
- MeSH terms
- Adipose Tissue, Aldehydes, Animals, Insulin Resistance, Male, Mice, Inbred C57BL, Obesity, Phenols, Biomarkers, Anti-Obesity Agents, 3T3-L1 Cells, Adiposity, Adipogenesis, Glucose Transporter Type 4, Mice, Diet, High-Fat
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