Wheat Extract Oil (WEO) Attenuates UVB-Induced Photoaging via Collagen Synthesis in Human Keratinocytes and Hairless Mice

Son, Dong Ju; Jung, Jae Chul; Choi, Yong Min; Ryu, Hyeon Yeol; Lee, Somin; Davis, Barbara A.

doi:10.3390/nu12020300

Cited by 26Skin Protection and Agingmelanin and skin pigmentationDermatology and Skin Diseases

Wheat Extract Oil (WEO) Attenuates UVB-Induced Photoaging via Collagen Synthesis in Human Keratinocytes and Hairless Mice

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Son, Dong Ju, Jung, Jae Chul, Choi, Yong Min, Ryu, Hyeon Yeol, Lee, Somin, Davis, Barbara A.·2020·Nutrients

DOI 10.3390/nu12020300PMC PMC7070621

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Abstract

The efficacy of wheat extract oil (WEO), standardized to glucosylceramides, for protecting against ultraviolet B (UVB)-induced damage of skin barrier function was assessed using the SHK-1 hairless mouse model and two human skin cell lines, namely, CCD-986sk and HeCaT. The ability of repeated oral administration of 30, 60, and 120 mg of WEO/kg/day for 12 weeks to prevent skin damage of SKH-1 hairless mice induced by UVB irradiation was evaluated. The results demonstrated that UVB-induced water evaporation (transepidermal water loss, TEWL) was significantly decreased by WEO. Similarly, UVB-induced losses in moisture and skin elasticity were improved by WEO supplementation. WEO attenuated the tissue procollagen type I, hyaluronic acid (HA), and ceramide reductions induced by UVB treatment as well. Collagen concentrations in skin tissue were increased in the WEO-treated mice, while UVB-induced epidermal thickening was reduced. In vitro studies using HeCaT human keratinocytes confirmed increased HA and collagen synthesis in response to WEO treatment. This may occur via WEO suppression of matrix metalloproteinase-1 (MMP-1), since its induction by UVB treatment was diminished in treated CCD-986sk cells. Oral administration of WEO improves skin barrier function in UVB-irradiated mice by attenuating damage typically observed in photoaging. This research further clarifies the clinical benefits previously observed by dietary WEO consumption.

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Disruption of the skin barrier and reduction in essential moisturizing factors such as hyaluronic acid and ceramides are hallmark features of UVB-induced photoaging.
“Disruption of the skin barrier and reduction in essential moisturizing factors such as hyaluronic acid and ceramides are hallmark features of UVB-induced photoaging [27].”
From Oral Centella asiatica Extract Attenuates UVB-Induced Skin Photoaging via Antioxidant, Anti-Inflammatory, and Extracellular Matrix-Preserving Effects in Hairless Mice (2025) · cites this paper as [27]
The Results (§3.1; page not provided) report reduced skin hydration and increased water loss after UVB exposure, and the Discussion (§4) reports a UVB-induced decrease in hyaluronic acid synthesis in keratinocytes. But the Results (§3.4) explicitly say the study did not observe a UVB-related change in epidermal ceramide content. The source therefore does not support presenting reductions in both hyaluronic acid and ceramides as hallmark features of UVB-induced photoaging. (LLM, conf=0.88)
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Wheat extract oil supplementation improved UVB-induced losses in skin moisture, elasticity, and damage of skin barrier function, and inhibited decreases in procollagen type I, HA, and ceramide, in mouse dorsal skin tissue.
“Wheat extract oil supplementation improved UVB-induced losses in skin moisture, elasticity, and damage of skin barrier function, and inhibited the decreases in procollagen type I, HA, and ceramide, in mouse dorsal skin tissue [120].”
From Boosting the Photoaged Skin: The Potential Role of Dietary Components (2021) · cites this paper as [120]

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Molecular Mechanisms of Stress-Responsive Changes in Collagen and Elastin Networks in Skin
Aziz, J., Shezali, H., Radzi, Z., Yahya, N. A., Abu Kassim, N. H., Czernuszka, J., & Rahman, M. T. (2016) · Skin Pharmacology and Physiology · doi:10.1159/000447017 · Cited by 7,323 · see citing works
UV-light-induced signal cascades and skin aging
Rittié, L. (2002) · Ageing Research Reviews · doi:10.1016/s1568-1637(02)00024-7 · Cited by 987 · see citing works
Molecular Mechanisms of Dermal Aging and Antiaging Approaches
Shin, J.-W., Kwon, S.-H., Choi, J.-Y., Na, J.-I., Huh, C.-H., Choi, H.-R., & Park, K.-C. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20092126 · Cited by 875 · see citing works
Skin hydration: A review on its molecular mechanisms
Verdier‐Sévrain, S., & Bonté, F. (2007) · Journal of Cosmetic Dermatology · doi:10.1111/j.1473-2165.2007.00300.x · Cited by 605 · see citing works
Skin barrier immunity and ageing
Chambers, E. S., & Vukmanovic‐Stejic, M. (2019) · Immunology · doi:10.1111/imm.13152 · Cited by 505 · see citing works
Solar Ultraviolet Irradiation Reduces Collagen in Photoaged Human Skin by Blocking Transforming Growth Factor-β Type II Receptor/Smad Signaling
Quan, T., He, T., Kang, S., Voorhees, J. J., & Fisher, G. J. (2004) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)63337-8 · Cited by 386 · see citing works
Ceramides of pig epidermis: structure determination
Wertz, P. W., & Downing, D. T. (1983) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)37950-5 · Cited by 356 · see citing works
Procollagen type I carboxy-terminal extension peptide in serum as a marker of collagen biosynthesis in bone. Correlation with iliac bone formation rates and comparison with total alkaline phosphatase
PARFITT, A. M., Simon, L. S., Villanueva, A. R., & Krane, S. M. (1987) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650020510 · Cited by 329 · see citing works
Importance of intercellular lipids in water-retention properties of the stratum corneum: induction and recovery study of surfactant dry skin
Imokawa, G., Akasaki, S., Minematsu, Y., & Kawai, M. (1989) · Archives of Dermatological Research · doi:10.1007/bf00424272 · Cited by 251 · see citing works
Absorption and lipoprotein transport of sphingomyelin
Nilsson, Å., & Duan, R. (2005) · Journal of Lipid Research · doi:10.1194/jlr.m500357-jlr200 · Cited by 246 · see citing works
Stratum Corneum Lipids Serve as a Bound-Water Modulator
Imokawa, G., Kuno, H., & Kawai, M. (1990) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12474562 · Cited by 239 · see citing works
HYALURONIC ACID IN CUTANEOUS INTRINSIC AGING
Ghersetich, I., Lotti, T., Campanile, G., Grappone, C., & Dini, G. (1994) · International Journal of Dermatology · doi:10.1111/j.1365-4362.1994.tb01540.x · Cited by 210 · see citing works
Evidence for Procollagen, a Biosynthetic Precursor of Collagen
Bellamy, G., & BORNSTEIN, P. K. (1971) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.68.6.1138 · Cited by 194 · see citing works
Trends in stratum corneum research and the management of dry skin conditions
Rawlings, A. V. (2003) · International Journal of Cosmetic Science · doi:10.1046/j.1467-2494.2003.00174.x · Cited by 194 · see citing works
Effect of lipid-containing, positively charged nanoemulsions on skin hydration, elasticity and erythema—An in vivo study
Yilmaz, E., & Borchert, H. (2005) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2005.10.002 · Cited by 185 · see citing works
The structure, function, and importance of ceramides in skin and their use as therapeutic agents in skin-care products
Meckfessel, M., & Brandt, S. (2014) · Journal of the American Academy of Dermatology · doi:10.1016/j.jaad.2014.01.891 · Cited by 178 · see citing works
UVB-Induced Alterations in Permeability Barrier Function: Roles for Epidermal Hyperproliferation and Thymocyte-Mediated Response
Haratake, A., Uchida, Y., Schmuth, M., Tanno, O., Yasuda, R., Epstein, J. H., Elias, P. M., & Holleran, W. M. (1997) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12292163 · Cited by 172 · see citing works
Anti-Wrinkle and Anti-Inflammatory Effects of Active Garlic Components and the Inhibition of MMPs via NF-κB Signaling
Kim, S. R., Jung, Y. R., An, H. J., Kim, D. H., Jang, E. J., Choi, Y. J., Moon, K. M., Park, M. H., Park, C. H., Chung, K. W., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0073877 · Cited by 161 · see citing works
Self-citation
Lipids and skin barrier function – a clinical perspective
Jungersted, J. M., Hellgren, L. I., Jemec, G. B. E., & Agner, T. (2008) · Contact Dermatitis · doi:10.1111/j.1600-0536.2008.01320.x · Cited by 148 · see citing works
Analysis of beta-glucocerebrosidase and ceramidase activities in atopic and aged dry skin.
Jin, K., Higaki, Y., Takagi, Y., Higuchi, K., Yada, Y., Kawashima, M., & Imokawa, G. (1994) · Acta Dermato Venereologica · doi:10.2340/0001555574341343 · Cited by 145 · see citing works
Active ingredients against human epidermal aging
Lorencini, M., Brohem, C. A., Dieamant, G., Zanchin, N. I. T., & Maïbach, H. I. (2014) · Ageing Research Reviews · doi:10.1016/j.arr.2014.03.002 · Cited by 139 · see citing works
Potential Role of Natural Compounds Against Skin Aging
Tundis, R., Loizzo, M. R., Bonesi, M., & Menichini, F. (2015) · Current Medicinal Chemistry · doi:10.2174/0929867322666150227151809 · Cited by 119 · see citing works
The role of ceramides in skin homeostasis and inflammatory skin diseases
Li, Q., Fang, H., Dang, E., & Wang, G. (2020) · Journal of Dermatological Science · doi:10.1016/j.jdermsci.2019.12.002 · Cited by 115 · see citing works
Quantitative Analysis of Stratum Corneum Lipids in Xerosis and Asteatotic Eczema
Akimoto, K., Yoshikawa, N., Higaki, Y., et al. (1993) · The Journal of Dermatology · doi:10.1111/j.1346-8138.1993.tb03820.x · Cited by 108 · see citing works
Dapsone in lichen planus.
Kumar, B., Kaur, I., & Bhattacharya, M. (1994) · Acta Dermato Venereologica · doi:10.2340/0001555574337340 · Cited by 104 · see citing works
Digestion of Maize Sphingolipids in Rats and Uptake of Sphingadienine by Caco-2 Cells
Sugawara, T., Kinoshita, M., Ohnishi, M., et al. (2003) · Journal of Nutrition · doi:10.1093/jn/133.9.2777 · Cited by 102 · see citing works
Importance of Sphingolipids and Inhibitors of Sphingolipid Metabolism as Components of Animal Diets
Merrill, A. H., Schmelz, E.-M., Wang, E., et al. (1997) · Journal of Nutrition · doi:10.1093/jn/127.5.830s · Cited by 98 · see citing works
Ultraviolet B‐induced alterations of the skin barrier and epidermal calcium gradient
Jiang, S. J., Chu, A. W., Lu, Z. F., Pan, M., Fa, D., & Zhou, X. (2007) · Experimental Dermatology · doi:10.1111/j.1600-0625.2007.00619.x · Cited by 78 · see citing works
Optimization of normal phase chromatographic conditions for lipid analysis and comparison of associated detection techniques
Abreu, S., Solgadi, A., & Chaminade, P. (2017) · Journal of Chromatography A · doi:10.1016/j.chroma.2017.07.063 · Cited by 71 · see citing works
Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts
Bi (2009) · International Journal of Molecular Medicine · doi:10.3892/ijmm_00000164 · Cited by 50 · see citing works
Nutrition and skin
Pappas, A., Liakou, A. Ι., & Zouboulis, C. C. (2016) · Reviews in Endocrine and Metabolic Disorders · doi:10.1007/s11154-016-9374-z · Cited by 46 · see citing works
Ceramides and the stratum corneum: structure, function, and new methods to promote repair
Goldstein, A. M., & Abramovits, W. (2003) · International Journal of Dermatology · doi:10.1046/j.1365-4362.2003.01507.x · Cited by 45 · see citing works
The moisturizing effect of a wheat extract food supplement on women’s skin: a randomized, double‐blind placebo‐controlled trial
Guillou, S., Ghabri, S., Jannot, C., Gaillard, E., Lamour, I., & Boisnic, S. (2010) · International Journal of Cosmetic Science · doi:10.1111/j.1468-2494.2010.00600.x · Cited by 44 · see citing works
Xerosis
Barco, D., & Giménez‐Arnau, A. M. (2008) · Actas Dermo-Sifiliográficas · doi:10.1016/s0001-7310(08)76171-4 · Cited by 42 · see citing works
Xerosis: a Dysfunction of the Epidermal Barrier
Barco, D., & Giménez-Arnau, A. M. (2008) · Actas Dermo-Sifiliográficas · doi:10.1016/s1578-2190(08)70343-3 · Cited by 34 · see citing works
Altered epidermal lipid layers induced by long‐term exposure to suberythemal‐dose ultraviolet
Bak, H., Hong, S., Jeong, S., et al. (2011) · International Journal of Dermatology · doi:10.1111/j.1365-4632.2010.04789.x · Cited by 30 · see citing works
Stimulatory Effect of Dietary Red Ginseng on Epidermal Hydration and Ceramide Levels in Ultraviolet-Irradiated Hairless Mice
Kim, H., Oh, I., Park, K., et al. (2009) · Journal of Medicinal Food · doi:10.1089/jmf.2008.1185 · Cited by 30 · see citing works
Analysis of beta-glucocerebrosidase and ceramidase activities in atopic and aged dry skin
神, 久. (1995) · Institutional Repositories DataBase (IRDB) · doi:10.11501/3105338 · Cited by 25 · see citing works
Polar lipids from wheat extract oil improve skin damages induced by aging: Evidence from a randomized, placebo‐controlled clinical trial in women and an ex vivo study on human skin explant
Boisnic, S., Kéophiphath, M., Sérandour, A., Branchet, M., Breton, S. L., Lamour, I., & Gaillard, E. (2019) · Journal of Cosmetic Dermatology · doi:10.1111/jocd.12967 · Cited by 16 · see citing works
Analyse biochimique des lipides épidermiques
Martini, M. (2003) · Pathologie Biologie · doi:10.1016/s0369-8114(03)00072-5 · Cited by 11 · see citing works
Les lipides cutanés : de la physiologie à la clinique
Claudy, A. (2003) · Pathologie Biologie · doi:10.1016/s0369-8114(03)00067-1 · Cited by 9 · see citing works
A very promising new glucolipidic surfactant: LipowheatTM
Djedour, A., Lafforgue, C., Marty, J. ‐., et al. (2005) · International Journal of Cosmetic Science · doi:10.1111/j.1467-2494.2005.00277.x · Cited by 3 · see citing works
An Experimental Study on the Effect of Angelica gigas Ethanol Extract on Hyaluronic Acid Synthesis
Park, H.-S., Ha, H.-Y., & Kim, H.-T. (2015) · The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology · doi:10.6114/jkood.2018.31.1.032 · Cited by 2 · see citing works

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Authors: Son, D. J., Jung, J. C., Choi, Y. M., Ryu, H. Y., Lee, S., & Davis, B. A.
Affiliations: Chungbuk National University (KR)
Novarex (South Korea) (KR)
Korea Conformity Laboratories, 8, Gaetbeol-ro 145 beon-gil, Yeonsu-gu, Incheon 21999, Korea
PLT Health Solutions Inc., 119 Headquarters Plaza, Morristown, NJ 07960, USA
Corresponding author: Davis, Barbara A.
ORCID: Son, Dong Ju
Choi, Yong Min
Ryu, Hyeon Yeol
Lee, Somin
Davis, Barbara A.
Venue: Nutrients
Publisher: MDPI AG
Published: 2020-01-22
Volume: 12
Issue: 2
Pages: 300
ISSN: 2072-6643 (ISSN-L)
DOI: 10.3390/nu12020300
PMC: PMC7070621
OpenAlex: W3001915759
Cited by: 26
References: 43
Citations by year: 2026: 2 · 2025: 7 · 2024: 4 · 2023: 5 · 2022: 4 · 2021: 3 · 2020: 1
FWCI: 2.37
Type: journal article
Language: en
Open access: gold
dx.doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0/ (vor)
Locations: Nutrients (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics: Health Sciences › Medicine › Dermatology › Skin Protection and Aging
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
Keywords: glucosylceramide, UVB-induced photoaging, skin barrier function, collagen synthesis, keratinocytes, transepidermal water loss, hyaluronic acid, ceramide, oral supplementation, hairless mice, epidermal thickening
MeSH terms: Animals, Collagen, Humans, Mice, Hairless, Photosensitivity Disorders, Plant Oils, Ultraviolet Rays, Triticum, Skin Aging, Keratinocytes, Mice

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Wheat Extract Oil (WEO) Attenuates UVB-Induced Photoaging via Collagen Synthesis in Human Keratinocytes and Hairless Mice

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