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Omega-3 Fatty Acid Supplementation on Post-Exercise Inflammation, Muscle Damage, Oxidative Response, and Sports Performance in Physically Healthy Adults—A Systematic Review of Randomized Controlled Trials

Fernández-Lázaro, Diego, Arribalzaga, Soledad, Gutiérrez-Abejón, Eduardo, Azarbayjani, Mohammad Ali, Mielgo-Ayuso, Juan, Roche, Enrique·2024·Nutrients
DOI 10.3390/nu16132044
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Abstract

Omega-3 is a family of n-3 polyunsaturated fatty acids (PUFAs), which have been used to treat a wide variety of chronic diseases, due mainly to their antioxidant and anti-inflammatory properties, among others. In this context, omega-3 could be post-exercise recovery agent and sports supplement that could improve performance by preserving and promoting skeletal muscle mass and strength. No conclusive evidence, however, exists about the potential effects of omega-3 on post-exercise biomarkers and sports performance in physically healthy adults. Based on the PRISMA in Exercise, Rehabilitation, Sports Medicine, and Sports Science (PERSiST) guidelines, we systematically reviewed studies indexed in Web of Science, Scopus, and Medline to assess the effects of omega-3 on post-exercise inflammation, muscle damage, oxidant response, and sports performance in physically healthy adults. The search was performed on original articles published in the last 10 years up to 5 May 2024, with a controlled trial design in which omega-3 supplementation was compared with a control group. Among 14,971 records identified in the search, 13 studies met the selection criteria. The duration of the interventions ranged from 1 day to 26 weeks of supplementation and the doses used were heterogeneous. Creatine kinase (CK) and lactate dehydrogenase (LDH) were significantly higher (p < 0.05) in the control group in 3 of the 4 studies where these markers were analyzed. C-reactive protein (CRP) was significantly higher (p < 0.05) in the control group of 2 of the 13 studies where this marker was analyzed. The delayed onset muscle soreness (DOMS) gave mixed results. Interleukin 6 (IL-6) showed improvements with supplementation, but tumor necrosis factor-α (TNF-α) displayed no differences. The consumption of n-3 PUFAs improved some indicators of oxidative stress such as reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio. Additional evidence is needed to establish clear recommendations regarding the dose and length of n-3 PUFA supplements. These may benefit the post-exercise inflammatory response, mitigate muscle damage, and decrease oxidative stress caused by exercise. However, studies did not evaluate omega-3 status at baseline or following supplementation and therefore the observations must be treated with caution

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Research works printed but not linked: 69

  1. Fernández-Lázaro, D., Mielgo-Ayuso, J., Calvo, J.S., Martínez, A.C., García, A.C., and Fernandez-Lazaro, C.I. (2020). Modulation of Exercise-Induced Muscle Damage, Inflammation, and Oxidative Markers by Curcumin Supplementation in a Physically Active Population: A Systematic Review. Nutrients, 12. · DOI
  2. Mittal, 2014, Reactive Oxygen Species in Inflammation and Tissue Injury, Antioxid. Redox Signal., 20, 1126, 10.1089/ars.2012.5149 · DOI · Paper page
  3. Biswas, 2016, Does the Interdependence between Oxidative Stress and Inflammation Explain the Antioxidant Paradox?, Oxidative Med. Cell. Longev., 2016, 17, 10.1155/2016/5698931 · DOI · Paper page
  4. Kern, 2018, Systemic and Airway Oxidative Stress in Competitive Swimmers, Respir. Med., 137, 129, 10.1016/j.rmed.2018.03.005 · DOI
  5. Reid, 2001, Nitric oxide, reactive oxygen species, and skeletal muscle contraction, Med. Sci. Sports Exerc., 33, 371, 10.1097/00005768-200103000-00006 · DOI
  6. Miguel, A.M.C.S., Roche, E., Herranz-López, M., Miguel, M.C.S., Mielgo-Ayuso, J., and Fernández-Lázaro, D. (2024). Impact of Melatonin Supplementation on Sports Performance and Circulating Biomarkers in Highly Trained Athletes: A Systematic Review of Randomized Controlled Trials. Nutrients, 16. · DOI
  7. Mielgo-Ayuso, J., and Fernández-Lázaro, D. (2021). Nutrition and Muscle Recovery. Nutrients, 13. · DOI
  8. Zaboli, 2012, Genetic Adaptation of Fatty-Acid Metabolism: A Human-Specific Haplotype Increasing the Biosynthesis of Long-Chain Omega-3 and Omega-6 Fatty Acids, Am. J. Hum. Genet., 90, 809, 10.1016/j.ajhg.2012.03.014 · DOI · Paper page
  9. Black, 2018, Adding Omega-3 Fatty Acids to a Protein-Based Supplement during Pre-Season Training Results in Reduced Muscle Soreness and the Better Maintenance of Explosive Power in Professional Rugby Union Players, Eur. J. Sport. Sci., 18, 1357, 10.1080/17461391.2018.1491626 · DOI
  10. Mickleborough, 2006, Protective Effect of Fish Oil Supplementation on Exercise-Induced Bronchoconstriction in Asthma, Chest, 129, 39, 10.1378/chest.129.1.39 · DOI
  11. Rees, 2006, Dose-Related Effects of Eicosapentaenoic Acid on Innate Immune Function in Healthy Humans: A Comparison of Young and Older Men, Am. J. Clin. Nutr., 83, 331, 10.1093/ajcn/83.2.331 · DOI · Paper page
  12. Smith, 2015, Fish Oil-Derived n-3 PUFA Therapy Increases Muscle Mass and Function in Healthy Older Adults, Am. J. Clin. Nutr., 102, 115, 10.3945/ajcn.114.105833 · DOI · Paper page
  13. Tinsley, 2017, Effects of Fish Oil Supplementation on Postresistance Exercise Muscle Soreness, J. Diet. Suppl., 14, 89, 10.1080/19390211.2016.1205701 · DOI
  14. Lembke, 2014, Influence of Omega-3 (N3) Index on Performance and Wellbeing in Young Adults after Heavy Eccentric Exercise, J. Sports Sci. Med., 3, 151
  15. Tsuchiya, 2016, Eicosapentaenoic and Docosahexaenoic Acids-Rich Fish Oil Supplementation Attenuates Strength Loss and Limited Joint Range of Motion after Eccentric Contractions: A Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Trial, Eur. J. Appl. Physiol., 116, 1179, 10.1007/s00421-016-3373-3 · DOI
  16. Kumar, 2004, Nuclear Factor-KappaB: Its Role in Health and Disease, J. Mol. Med., 82, 434, 10.1007/s00109-004-0555-y · DOI · Paper page
  17. Ochi, 2017, Effect of eicosapentaenoic acids-rich fish oil supplementation on motor nerve function after eccentric contractions, J. Int. Soc. Sports Nutr., 14, 23, 10.1186/s12970-017-0176-9 · DOI
  18. Tartibian, 2011, Omega-3 Fatty Acids Supplementation Attenuates Inflammatory Markers after Eccentric Exercise in Untrained Men, Clin. J. Sport Med., 21, 131, 10.1097/JSM.0b013e31820f8c2f · DOI
  19. Gladman, 2012, Improved Outcome after Peripheral Nerve Injury in Mice with Increased Levels of Endogenous ω-3 Polyunsaturated Fatty Acids, J. Neurosci. Off. J. Soc. Neurosci., 32, 563, 10.1523/JNEUROSCI.3371-11.2012 · DOI
  20. Patten, 2002, Dietary Fish Oil Increases Acetylcholine- and Eicosanoid-Induced Contractility of Isolated Rat Ileum, J. Nutr., 132, 2506, 10.1093/jn/132.9.2506 · DOI
  21. Tartibian, 2009, The Effects of Ingestion of Omega-3 Fatty Acids on Perceived Pain and External Symptoms of Delayed Onset Muscle Soreness in Untrained Men, Clin. J. Sport Med., 19, 115, 10.1097/JSM.0b013e31819b51b3 · DOI
  22. Biolo, 1997, An Abundant Supply of Amino Acids Enhances the Metabolic Effect of Exercise on Muscle Protein, Am. J. Physiol., 273, E122
  23. Lewis, 2015, 21 days of mammalian omega-3 fatty acid supplementation improves aspects of neuromuscular function and performance in male athletes compared to olive oil placebo, J. Int. Soc. Sports Nutr., 12, 28, 10.1186/s12970-015-0089-4 · DOI
  24. Ardern, 2022, Implementing the 27 PRISMA 2020 Statement items for systematic reviews in the sport and exercise medicine, musculoskeletal rehabilitation and sports science fields: The PERSiST (implementing Prisma in Exercise, Rehabilitation, Sport medicine and SporTs science) guidance, Br. J. Sports Med., 56, 175, 10.1136/bjsports-2021-103987 · DOI · Paper page
  25. Law, M., Stewart, D., Letts, L., Pollock, N., Bosch, J., and Westmorland, M. (1998). Guidelines for Critical Review of Qualitative Studies, McMaster University Occupational Therapy Evidence-Based Practice Research Group.
  26. Moseley, 2020, Using research to guide practice: The Physiotherapy Evidence Database (PEDro). Braz, J. Phys. Ther., 24, 384
  27. Tomczyk, 2024, Omega-3 fatty acid supplementation affects tryptophan metabolism during a 12-week endurance training in amateur runners: A randomized controlled trial, Sci. Rep., 14, 4102, 10.1038/s41598-024-54112-x · DOI
  28. Tsuchiya, 2021, 4-Week Eicosapentaenoic Acid-Rich Fish Oil Supplementation Partially Protects Muscular Damage Following Eccentric Contractions, J. Int. Soc. Sports Nutr., 18, 18, 10.1186/s12970-021-00411-x · DOI
  29. Vandusseldorp, T.A., Escobar, K.A., Johnson, K.E., Stratton, M.T., Moriarty, T., Kerksick, C.M., Mangine, G.T., Holmes, A.J., Lee, M., and Endito, M.R. (2020). Impact of Varying Dosages of Fish Oil on Recovery and Soreness Following Eccentric Exercise. Nutrients, 12. · DOI
  30. 2020, Re-esterified DHA improves ventilatory threshold 2 in competitive amateur cyclists, J. Int. Soc. Sports Nutr., 17, 51, 10.1186/s12970-020-00379-0 · DOI
  31. Barquilha, G., Miguel, C., Dos, M., Caçula, K.G., Polotow, T.G., Vasconcellos, C.V., Fernandes, A., Rodrigues, L.E., Lambertucci, R.H., and Duarte, T. (2023). Fish Oil Supplementation Improves the Repeated-Bout Effect Strength Training. Nutrients, 15. · DOI
  32. Brook, 2021, Omega-3 supplementation during unilateral resistance exercise training in older women: A within subject and double-blind placebo-controlled trial, Clin. Nutr. ESPEN, 46, 394, 10.1016/j.clnesp.2021.09.729 · DOI
  33. Heileson, 2023, The effect of fish oil supplementation on resistance training-induced adaptations, J. Int. Soc. Sports Nutr., 20, 2174704, 10.1080/15502783.2023.2174704 · DOI
  34. Jakeman, 2017, Effect of an Acute Dose of Omega-3 Fish Oil Following Exercise-Induced Muscle Damage, Eur. J. Appl. Physiol., 117, 575, 10.1007/s00421-017-3543-y · DOI
  35. Lee, 2022, V Fish Oil Administration Combined with Resistance Exercise Training Improves Strength, Resting Metabolic Rate, and Inflammation in Older Adults, Aging Clin. Exp. Res., 34, 3073, 10.1007/s40520-022-02250-5 · DOI
  36. Mullins, V.A., Graham, S., Cummings, D., Wood, A., Ovando, V., Skulas-Ray, A.C., Polian, D., Wang, Y., Hernandez, G.D., and Lopez, C.M. (2022). Effects of Fish Oil on Biomarkers of Axonal Injury and Inflammation in American Football Players: A Placebo-Controlled Randomized Controlled Trial. Nutrients, 14. · DOI
  37. Nieman, 2015, No Positive Influence of Ingesting Chia Seed Oil on Human Running Performance, Nutrients, 7, 3666, 10.3390/nu7053666 · DOI
  38. Hou, 2016, Omega-3 fatty acids, lipid rafts, and T cell signaling, Eur. J. Pharmacol., 785, 2, 10.1016/j.ejphar.2015.03.091 · DOI
  39. Ramos-Campo, D.J., Ávila-Gandía, V., López-Román, F.J., Miñarro, J., Contreras, C., Soto-Méndez, F., Domingo Pedrol, J.C., and Luque-Rubia, A.J. (2020). Supplementation of Re-Esterified Docosahexaenoic and Eicosapentaenoic Acids Reduce Inflammatory and Muscle Damage Markers after Exercise in Endurance Athletes: A Randomized, Controlled Crossover Trial. Nutrients, 12. · DOI
  40. Yang, 2023, Impact of Antarctic krill oil supplementation on skeletal muscle injury recovery after resistance exercise, Eur. J. Nutr., 62, 1345, 10.1007/s00394-022-03077-6 · DOI
  41. Kyriakidou, 2021, The Effect of Omega-3 Polyunsaturated Fatty Acid Supplementation on Exercise-Induced Muscle Damage, J. Int. Soc. Sports Nutr., 18, 9, 10.1186/s12970-020-00405-1 · DOI
  42. Lange, 2019, Are there serious adverse effects of omega-3 polyunsaturated fatty acid supplements?, J. Food Bioact., 7, 1, 10.31665/JFB.2019.7192 · DOI
  43. DiLorenzo, 2014, Docosahexaenoic Acid Affects Markers of Inflammation and Muscle Damage after Eccentric Exercise, J. Strength Cond. Res., 28, 2768, 10.1519/JSC.0000000000000617 · DOI
  44. Canals-Garzón, C., Guisado-Barrilao, R., Martínez-García, D., Chirosa-Ríos, I.J., Jerez-Mayorga, D., and Guisado-Requena, I.M. (2022). Effect of Antioxidant Supplementation on Markers of Oxidative Stress and Muscle Damage after Strength Exercise: A Systematic Review. Int. J. Environ. Res. Public Health, 19. · DOI
  45. Bloomer, 2009, Effect of Eicosapentaenoic and Docosahexaenoic Acid on Resting and Exercise-Induced Inflammatory and Oxidative Stress Biomarkers: A Randomized, Placebo Controlled, Cross-over Study, Lipids Health Dis., 8, 36, 10.1186/1476-511X-8-36 · DOI
  46. Gray, 2014, Fish Oil Supplementation Reduces Markers of Oxidative Stress but Not Muscle Soreness after Eccentric Exercise, Int. J. Sport. Nutr. Exerc. Metab., 24, 206, 10.1123/ijsnem.2013-0081 · DOI
  47. Da Boit, M., Mastalurova, I., Brazaite, G., McGovern, N., Thompson, K., and Gray, S.R. (2015). The Effect of Krill Oil Supplementation on Exercise Performance and Markers of Immune Function. PLoS ONE, 10. · DOI
  48. Diefenthaeler, 2015, Benefits of Dietary Phytochemical Supplementation on Eccentric Exercise-Induced Muscle Damage: Is Including Antioxidants Enough?, Nutrition, 31, 1072, 10.1016/j.nut.2015.02.014 · DOI
  49. Philpott, 2018, Adding Fish Oil to Whey Protein, Leucine, and Carbohydrate Over a Six-Week Supplementation Period Attenuates Muscle Soreness Following Eccentric Exercise in Competitive Soccer Players, Int. J. Sport. Nutr. Exerc. Metab., 28, 26, 10.1123/ijsnem.2017-0161 · DOI
  50. Chalchat, E., Gaston, A.-F., Charlot, K., Peñailillo, L., Valdés, O., Tardo-Dino, P.-E., Nosaka, K., Martin, V., Garcia-Vicencio, S., and Siracusa, J. (2022). Appropriateness of Indirect Markers of Muscle Damage Following Lower Limbs Eccentric-Biased Exercises: A Systematic Review with Meta-Analysis. PLoS ONE, 17. · DOI
  51. Lv, Z., Zhang, J., and Zhu, W. (2020). Omega-3 Polyunsaturated Fatty Acid Supplementation for Reducing Muscle Soreness after Eccentric Exercise: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Biomed. Res. Int., 2020. · DOI
  52. Mason, S.A., Trewin, A.J., Parker, L., and Wadley, G.D. (2020). Antioxidant Supplements and Endurance Exercise: Current Evidence and Mechanistic Insights. Redox Biol., 35. · DOI · Paper page
  53. Fayh, 2018, Effects of N-3 Fatty Acids and Exercise on Oxidative Stress Parameters in Type 2 Diabetic: A Randomized Clinical Trial, J. Int. Soc. Sports Nutr., 15, 18, 10.1186/s12970-018-0222-2 · DOI
  54. Buonocore, 2020, Effect of 8-Week n-3 Fatty-Acid Supplementation on Oxidative Stress and Inflammation in Middle- and Long-Distance Running Athletes: A Pilot Study, J. Int. Soc. Sports Nutr., 17, 55, 10.1186/s12970-020-00391-4 · DOI
  55. Drobnic, F., Storsve, A.B., Burri, L., Ding, Y., Banquells, M., Riera, J., Björk, P., Ferrer-Roca, V., and Domingo, J.C. (2021). Krill-Oil-Dependent Increases in HS-Omega-3 Index, Plasma Choline and Antioxidant Capacity in Well-Conditioned Power Training Athletes. Nutrients, 13. · DOI
  56. Berge, 2015, Krill Oil Reduces Plasma Triacylglycerol Level and Improves Related Lipoprotein Particle Concentration, Fatty Acid Composition and Redox Status in Healthy Young Adults—A Pilot Study, Lipids Health Dis., 14, 163, 10.1186/s12944-015-0162-7 · DOI · Paper page
  57. Marques, 2015, Effects of DHA-Rich Fish Oil Supplementation on the Lipid Profile, Markers of Muscle Damage, and Neutrophil Function in Wheelchair Basketball Athletes before and after Acute Exercise, Appl. Physiol. Nutr. Metab., 40, 596, 10.1139/apnm-2014-0140 · DOI
  58. Atashak, 2013, Effect of Omega-3 Supplementation On The Blood Levels Of Oxidative Stress, Muscle Damage Inflamm. Markers After Acute Resist., 45, 22
  59. De Salazar, L., Contreras, C., Torregrosa-García, A., Luque-Rubia, A.J., Ávila-Gandía, V., Domingo, J.C., and López-Román, F.J. (2020). Oxidative Stress in Endurance Cycling Is Reduced Dose-Dependently after One Month of Re-Esterified DHA Supplementation. Antioxidants, 18. · DOI
  60. Andre, 2018, Effectiveness of Fish Oil Supplementation in Attenuating Exercise-Induced Muscle Damage in Women During Midfollicular and Midluteal Menstrual Phases, J. Strength Cond. Res., 32, 1601, 10.1519/JSC.0000000000002247 · DOI
  61. Luostarinen, 1996, Dietary Fish Oil Decreases Superoxide Generation by Human Neutrophils: Relation to Cyclooxygenase Pathway and Lysosomal Enzyme Release, Prostaglandins Leukot. Essent. Fatty Acids, 55, 167, 10.1016/S0952-3278(96)90093-9 · DOI
  62. Fisher, 1990, Dietary N-3 Fatty Acid Supplementation Reduces Superoxide Production and Chemiluminescence in a Monocyte-Enriched Preparation of Leukocytes, Am. J. Clin. Nutr., 51, 804, 10.1093/ajcn/51.5.804 · DOI
  63. Lenn, 2002, The Effects of Fish Oil and Isoflavones on Delayed Onset Muscle Soreness, Med. Sci. Sports Exerc., 34, 1605, 10.1097/00005768-200210000-00012 · DOI
  64. Owen, 2010, Measurement of Oxidized/Reduced Glutathione Ratio, Methods Mol. Biol., 648, 269, 10.1007/978-1-60761-756-3_18 · DOI · Paper page
  65. Owens, 2019, Exercise-induced muscle damage: What is it, what causes it and what are the nutritional solutions?, Eur. J. Sport Sci., 19, 71, 10.1080/17461391.2018.1505957 · DOI · Paper page
  66. Ochi, E., and Tsuchiya, Y. (2018). Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) in Muscle Damage and Function. Nutrients, 10. · DOI
  67. Ross, 2008, The emerging role of eicosapentaenoic acid as an important psychoactive natural product: Some answers but a lot more questions, Lipid Insights, 2, 89, 10.4137/LPI.S1013 · DOI
  68. Fernández-Lázaro, D., Mielgo-Ayuso, J., Del Valle Soto, M., Adams, D.P., Gutiérrez-Abejón, E., and Seco-Calvo, J. (2021). Impact of Optimal Timing of Intake of Multi-Ingredient Performance Supplements on Sports Performance, Muscular Damage, and Hormonal Behavior across a Ten-Week Training Camp in Elite Cyclists: A Randomized Clinical Trial. Nutrients, 13. · DOI
  69. Fernández-Lázaro, D., Garrosa, E., Seco-Calvo, J., and Garrosa, M. (2022). Potential Satellite Cell-Linked Biomarkers in Aging Skeletal Muscle Tissue: Proteomics and Proteogenomics to Monitor Sarcopenia. Proteomes, 10. · DOI

Cited by

Metadata

Authors
Fernández-Lázaro, D., Arribalzaga, S., Gutiérrez-Abejón, E., Azarbayjani, M. A., Mielgo-Ayuso, J., & Roche, E.
Venue
Nutrients
Publisher
MDPI AG
Published
2024-06-27
Volume
16
Issue
13
Pages
2044
ISSN
2072-6643
DOI
10.3390/nu16132044
Cited by
92
References
10
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (vor)
Funders
Department of Education of the Junta de Castilla &amp; León and the European Regional Development Fund (ERDF) (067/230003)

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