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Characterization of mRNA and microRNA in human mast cell-derived exosomes and their transfer to other mast cells and blood CD34 progenitor cells
Open accessAbstract
Background: Exosomes are nanosized vesicles of endocytic origin that are released into the extracellular environment by many different cells. It has been shown that exosomes from various cellular origins contain a substantial amount of RNA (mainly mRNA and microRNA). More importantly, exosomes are capable of delivering their RNA content to target cells, which is a novel way of cell‐to‐cell communication. The aim of this study was to evaluate whether exosomal shuttle RNA could play a role in the communication between human mast cells and between human mast cells and human CD34 + progenitor cells. Methods: The mRNA and microRNA content of exosomes from a human mast cell line, HMC‐1, was analysed by using microarray technology. Co‐culture experiments followed by flow cytometry analysis and confocal microscopy as well as radioactive labeling experiments were performed to examine the uptake of these exosomes and the shuttle of the RNA to other mast cells and CD34 + progenitor cells. Results: In this study, we show that human mast cells release RNA‐containing exosomes, with the capacity to shuttle RNA between cells. Interestingly, by using microRNA microarray analysis, 116 microRNAs could be identified in the exosomes and 134 microRNAs in the donor mast cells. Furthermore, DNA microarray experiments revealed the presence of approximately 1800 mRNAs in the exosomes, which represent 15% of the donor cell mRNA content. In addition, transfer experiments revealed that exosomes can shuttle RNA between human mast cells and to CD34 + hematopoietic progenitor cells. Conclusion: These findings suggest that exosomal shuttle RNA (esRNA) can play a role in the communication between cells, including mast cells and CD34 + progenitor cells, implying a role in cells maturation process.
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“Recent studies indicate that they contain proteins, nucleic acids and miRNAs (Ekström et al., 2012; Costa-Silva et al., 2015; Simons and Raposo, 2009).”
From Tumor microenvironment derived exosomes pleiotropically modulate cancer cell metabolism (2016) · cites this paper as Ekström et al., 2012
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Metadata
- Authors
- Ekström, K., Valadi, H., Sjöstrand, M., Malmhäll, C., Bossios, A., Eldh, M., & Lötvall, J.
- Affiliations
- University of Gothenburg (SE)
- Corresponding author
- Lötvall, Jan
- Venue
- Journal of Extracellular Vesicles
- Publisher
- Wiley
- Published
- 2012-01
- Volume
- 1
- Issue
- 1
- ISSN
- 2001-3078 (ISSN-L)
- PMC
- PMC3760639
- OpenAlex
- W2132589340
- Cited by
- 201
- References
- 36
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 10 · 2023: 17 · 2022: 15 · 2021: 15 · 2020: 23 · 2019: 15 · 2018: 19 · 2017: 14 · 2016: 17 · 2015: 20 · 2014: 14 · 2013: 8 · 2012: 4
- FWCI
- 5.91
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Extracellular Vesicles (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Vetenskapsrådet (K2008‐57X‐20676‐01‐3)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Keywords
- exosomes, mast cells, microRNA, mRNA, cell-to-cell communication, CD34+ hematopoietic progenitor cells, HMC-1 cell line, extracellular vesicles, exosome uptake
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