The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Macrophage‐dependent clearance of systemically administered B16BL6‐derived exosomes from the blood circulation in mice
Open accessAbstract
Previous studies using B16BL6‐derived exosomes labelled with gLuc–lactadherin (gLuc‐LA), a fusion protein of Gaussia luciferase (a reporter protein) and lactadherin (an exosome‐tropic protein), showed that the exosomes quickly disappeared from the systemic circulation after intravenous injection in mice. In the present study, the mechanism of rapid clearance of intravenously injected B16BL6 exosomes was investigated. gLuc‐LA‐labelled exosomes were obtained from supernatant of B16BL6 cells after transfection with a plasmid DNA encoding gLuc‐LA. Labelling was stable when the exosomes were incubated in serum. By using B16BL6 exosomes labelled with PKH26, a lipophilic fluorescent dye, it was demonstrated that PKH26‐labelled B16BL6 exosomes were taken up by macrophages in the liver and spleen but not in the lung, while PKH26‐labelled exosomes were taken up by the endothelial cells in the lung. Subsequently, gLuc‐LA‐labelled B16BL6 exosomes were injected into macrophage‐depleted mice prepared by injection with clodronate‐containing liposomes. The clearance of the intravenously injected B16BL6 exosomes from the blood circulation was much slower in macrophage‐depleted mice than that in untreated mice. These results indicate that macrophages play important roles in the clearance of intravenously injected B16BL6 exosomes from the systemic circulation.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766
Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes
Alvarez-Erviti, L., Seow, Y., Yin, H., Betts, C., Lakhal, S., & Wood, M. J. A. (2011) · Nature Biotechnology · doi:10.1038/nbt.1807 · Cited by 4,930
Membrane vesicles as conveyors of immune responses
Théry, C., Ostrowski, M., & Segura, E. (2009) · Nature Reviews Immunology · doi:10.1038/nri2567 · Cited by 3,882
Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET
Peinado, H., Alečković, M., Lavotshkin, S., Matei, I., Costa-Silva, B., Moreno-Bueno, G., Hergueta-Redondo, M., Williams, C., García-Santos, G., Ghajar, C. M., et al. (2012) · Nature Medicine · doi:10.1038/nm.2753 · Cited by 3,715
Extracellular vesicles: biology and emerging therapeutic opportunities
EL Andaloussi, S., Mäger, I., Breakefield, X. O., & Wood, M. J. A. (2013) · Nature Reviews Drug Discovery · doi:10.1038/nrd3978 · Cited by 3,571
B lymphocytes secrete antigen-presenting vesicles.
Raposo, G., Nijman, H. W., Stoorvogel, W., Liejendekker, R., Harding, C. V., Melief, C. J., & Geuze, H. J. (1996) · The Journal of experimental medicine · doi:10.1084/jem.183.3.1161 · Cited by 3,546
Singh, R. P., Kurz, J., & Boiteau, G. (1996) · Journal of Virological Methods · doi:10.1016/0166-0934(96)02096-4 · Cited by 2,552
Rooijen, N. V., & Sanders, A. (1994) · Journal of Immunological Methods · doi:10.1016/0022-1759(94)90012-4 · Cited by 1,779
Statistical moments in pharmacokinetics
Yamaoka, K., Nakagawa, T., & Uno, T. (1978) · Journal of Pharmacokinetics and Biopharmaceutics · doi:10.1007/bf01062109 · Cited by 1,250
Endocytosis, intracellular sorting, and processing of exosomes by dendritic cells
Morelli, A. E., Larregina, A. T., Shufesky, W. J., Sullivan, M. L. G., Stolz, D. B., Papworth, G. D., Zahorchak, A. F., Logar, A. J., Wang, Z., Watkins, S. C., et al. (2004) · Blood · doi:10.1182/blood-2004-03-0824 · Cited by 1,073
Cellular Internalization of Exosomes Occurs Through Phagocytosis
Feng, D., Zhao, W.-L., Ye, Y.-Y., Bai, X.-C., Liu, R.-Q., Chang, L.-F., Zhou, Q., & Sui, S.-F. (2010) · Traffic · doi:10.1111/j.1600-0854.2010.01041.x · Cited by 981
Structural and functional aspects of liver sinusoidal endothelial cell fenestrae: a review
Braet, F., & Wisse, E. (2002) · Comparative Hepatology · doi:10.1186/1476-5926-1-1 · Cited by 753
Takahashi, Y., Nishikawa, M., Shinotsuka, H., Matsui, Y., Ohara, S., Imai, T., & Takakura, Y. (2013) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2013.03.013 · Cited by 728
Self-citationExosomes for drug delivery—A novel application for the mesenchymal stem cell
Lai, R. C., Yeo, R. W. Y., Tan, K. H., & Lim, S. K. (2013) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.08.008 · Cited by 546
Sheldon, H., Heikamp, E., Turley, H., Dragovic, R., Thomas, P., Oon, C. E., Leek, R., Edelmann, M., Kessler, B., Sainson, R. C. A., et al. (2010) · Blood · doi:10.1182/blood-2009-08-239228 · Cited by 410
CD169 mediates the capture of exosomes in spleen and lymph node
Saunderson, S. C., Dunn, A. C., Crocker, P. R., & McLellan, A. D. (2014) · Blood · doi:10.1182/blood-2013-03-489732 · Cited by 395
Liposomes for Specific Depletion of Macrophages from Organs and Tissues
van Rooijen, N., & Hendrikx, E. (2010) · Methods in Molecular Biology · doi:10.1007/978-1-60327-360-2_13 · Cited by 379
Ishida, T., Harashima, H., & Kiwada, H. (2002) · Bioscience Reports · doi:10.1023/a:1020134521778 · Cited by 330
Van Rooijen, N., Kors, N., Ende, M. V. D., & Dijkstra, C. D. (1990) · Cell and Tissue Research · doi:10.1007/bf00318625 · Cited by 262
Microvesicle-mediated RNA Molecule Delivery System Using Monocytes/Macrophages
Akao, Y., Iio, A., Itoh, T., Noguchi, S., Itoh, Y., Ohtsuki, Y., & Naoe, T. (2011) · Molecular Therapy · doi:10.1038/mt.2010.254 · Cited by 253
Reed, S. E., Staley, E. M., Mayginnes, J. P., Pintel, D. J., & Tullis, G. E. (2006) · Journal of Virological Methods · doi:10.1016/j.jviromet.2006.07.024 · Cited by 251
Nishikawa, K., Arai, H., & Inoue, K. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)34110-9 · Cited by 232
Tumor-exosomes and leukocyte activation: an ambivalent crosstalk
Zech, D., Rana, S., Büchler, M. W., & Zöller, M. (2012) · Cell Communication and Signaling · doi:10.1186/1478-811x-10-37 · Cited by 217
Organ-specific endothelial cell uptake of cationic liposome-DNA complexes in mice
McLean, J. W., Fox, E. A., Baluk, P., Bolton, P. B., Haskell, A., Pearlman, R., Thurston, G., Umemoto, E. Y., & McDonald, D. M. (1997) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.1997.273.1.h387 · Cited by 199
Exosome Display technology: Applications to the development of new diagnostics and therapeutics
Delcayre, A., Estelles, A., Sperinde, J., Roulon, T., Paz, P., Aguilar, B., Villanueva, J., Khine, S., & Le Pecq, J.-B. (2005) · Blood Cells, Molecules, and Diseases · doi:10.1016/j.bcmd.2005.07.003 · Cited by 190
Cited by
Extracellular vesicles for drug delivery.
Vader, P., Mol, E. A., Pasterkamp, G., & Schiffelers, R. M. (2016) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2016.02.006 · Cited by 1,319
Saari, H., Lázaro-Ibáñez, E., Viitala, T., Vuorimaa-Laukkanen, E., Siljander, P., & Yliperttula, M. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.09.031 · Cited by 613
Macrophage exosomes as natural nanocarriers for protein delivery to inflamed brain
Yuan, D., Zhao, Y., Banks, W. A., Bullock, K. M., Haney, M., Batrakova, E., & Kabanov, A. V. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.07.011 · Cited by 607
Exosomes and Exosome-Inspired Vesicles for Targeted Drug Delivery
Antimisiaris, S. G., Mourtas, S., & Marazioti, A. (2018) · doi:10.20944/preprints201810.0507.v1 · Cited by 605
Banks, W. A., Sharma, P., Bullock, K. M., Hansen, K. M., Ludwig, N., & Whiteside, T. L. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21124407 · Cited by 561
Metadata
- Authors
- Imai, T., Takahashi, Y., Nishikawa, M., Kato, K., Morishita, M., Yamashita, T., Matsumoto, A., Charoenviriyakul, C., & Takakura, Y.
- Venue
- Journal of Extracellular Vesicles
- Publisher
- Wiley
- Published
- 2015-01
- Volume
- 4
- Issue
- 1
- Pages
- 26238-26238
- ISSN
- 2001-3078 (ISSN-L)
- OpenAlex
- W2072153678
- Cited by
- 579
- References
- 25
- FWCI
- 11.45
- Type
- journal article
- Language
- en
- Open access
- gold
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
