Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay
Open accessAbstract
The pulmonary metastasis assay (PuMA) is an ex vivo lung explant and closed cell culture system that permits researchers to study the biology of lung colonization in osteosarcoma (OS) by fluorescence microscopy. This article provides a detailed description of the protocol, and discusses examples of obtaining image data on metastatic growth using widefield or confocal fluorescence microscopy platforms. The flexibility of the PuMA model permits researchers to study not only the growth of OS cells in the lung microenvironment, but also to assess the effects of anti-metastatic therapeutics over time. Confocal microscopy allows for unprecedented, high-resolution imaging of OS cell interactions with the lung parenchyma. Moreover, when the PuMA model is combined with fluorescent dyes or fluorescent protein genetic reporters, researchers can study the lung microenvironment, cellular and subcellular structures, gene function, and promoter activity in metastatic OS cells. The PuMA model provides a new tool for osteosarcoma researchers to discover new metastasis biology and assess the activity of novel anti-metastatic, targeted therapies.
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Ex vivo culture methods permit investigation of tumour cells that grow within an intact lung environment.
“Ex vivo culture systems are useful to study tumour cells growing within an intact lung environment 214,215 .”
From Osteosarcoma (2022) · cites this paper as [215]
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Self-citation
- Authors
- Lizardo, M. M., & Sorensen, P. H.
- Affiliations
- BC Cancer Agency (CA)
- Venue
- Journal of Visualized Experiments
- Publisher
- MyJove Corporation
- Published
- 2018-03-12
- Issue
- 133
- ISSN
- 1940-087X (ISSN-L)
- PMC
- PMC5931694
- OpenAlex
- W2794411119
- Cited by
- 19
- References
- 26
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 3 · 2018: 2
- FWCI
- 1.03
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Visualized Experiments (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- BC Cancer Agency
- National Cancer Institute
- Topics
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Sarcoma Diagnosis and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mechanisms of cancer metastasis
- Keywords
- osteosarcoma, lung colonization, metastasis biology, confocal fluorescence microscopy, lung microenvironment, fluorescent reporter genes
- MeSH terms
- Animals, Bone Neoplasms, Cell Communication, Female, Humans, Lung Neoplasms, Mice, Inbred BALB C, Neoplasm Metastasis, Osteosarcoma, Mice, SCID, Microscopy, Confocal, Cell Line, Tumor, Mice
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