Cited by 92Cancer Cells and MetastasisMechanisms of cancer metastasisCell Adhesion Molecules Research

Modeling metastasis biology and therapy in real time in the mouse lung

Open access
Mendoza, Arnulfo, Hong, Sung-Hyeok, Osborne, Tanasa, Khan, Mohammed A., Campbell, Kirk, Briggs, Joseph, Eleswarapu, Ananth, Buquo, Lauren +10 more·2010·Journal of Clinical Investigation

Abstract

Pulmonary metastasis remains the leading ca use of death for cancer patients. Opportunities to improve treatment outcomes for patients require new methods to study and view the biology of metastatic progression. Here, we describe an ex vivo pulmonary metastasis assay (PuMA) in which the metastatic progression of GFP-expressing cancer cells, from a single cell to the formation of multicellular colonies, in the mouse lung microenvironment was assessed in real time for up to 21 days. The biological validity of this assay was confirmed by its prediction of the in vivo behavior of a variety of high- and low-metastatic human and mouse cancer cell lines and the discrimination of tumor microenvironments in the lung that were most permissive to metastasis. Using this approach, we provide what we believe to be new insights into the importance of tumor cell interactions with the stromal components of the lung microenvironment. Finally, the translational utility of this assay was demonstrated through its use in the evaluation of therapeutics at discrete time points during metastatic progression. We believe that this assay system is uniquely capable of advancing our understanding of both metastasis biology and therapeutic strategies.

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Crossref · 10.1172/jci40252

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  • Ex vivo culture methods permit investigation of tumour cells that grow within an intact lung environment.

    “Ex vivo culture systems are useful to study tumour cells growing within an intact lung environment 214,215 .”

    From Osteosarcoma (2022) · cites this paper as [214]

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Authors
Mendoza, A., Hong, S.-H., Osborne, T., Khan, M. A., Campbell, K., Briggs, J., Eleswarapu, A., Buquo, L., Ren, L., Hewitt, S. M., Dakir, E.-H., Garfield, S., Walker, R., Merlino, G., Green, J. E., Hunter, K. W., Wakefield, L. M., & Khanna, C.
Affiliations
National Institutes of Health (US)
Corresponding author
Khanna, Chand
Venue
Journal of Clinical Investigation
Published
2010-08-02
Volume
120
Issue
8
Pages
2979-2988
ISSN
0021-9738 (ISSN-L)
OpenAlex
W1992891544
Cited by
92
References
25
Citations by year
2026: 1 · 2025: 3 · 2024: 6 · 2023: 6 · 2022: 5 · 2021: 7 · 2020: 9 · 2019: 3 · 2018: 7 · 2017: 8 · 2016: 7 · 2015: 9 · 2014: 3 · 2013: 7 · 2012: 4
FWCI
2.85
Type
journal article
Language
en
Open access
Locations
  • Journal of Clinical Investigation (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Howard Hughes Medical Institute
Topics
  • Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mechanisms of cancer metastasis
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
Keywords
lung metastasis, metastatic progression, lung microenvironment, tumor stroma, metastatic cell lines, real-time imaging, tumor spheroids
MeSH terms
Animals, Disease Models, Animal, Female, Humans, Lung Neoplasms, Mice, Inbred Strains, Disease Progression, Cell Line, Tumor, Mice

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