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Oncogenic function of angiopoietin-2 in vitro and its modulation of tumor progression in colorectal carcinoma

Kim, Hyungjoo, Ahn, Tae Sung, Kim, Chang-Jin, Bae, Sang Byung, Kim, Han Jo, Lee, Chang-Seuk, Kim, Tae Hyun, Im, Jungkyun, Lee, Sang Hun, Son, Myoung Won, Lee, Moon Soo, Baek, Moo Jun, Jeong, Dongjun·2017·Oncology Letters
DOI 10.3892/ol.2017.6203PMC PMC5494651
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Figure 1. Ang-2 expression was investigated by reverse-transcription polymerase chain reaction and western blot analysis in colorectal cancer cell lines, HUVEC and normal cell line (CCD-841 CoN). (A) Among 5 colorectal cancer cell lines, 3 cell lines expressed Ang-2 mRNA, as did the HUVEC cell line. (B) LoVo cells expressed a markedly higher level of Ang-2 protein compared with other colorectal cancer cell lines, as detected by western blotting. (C) The LoVo cell line transfected with Ang-2 target shRNA did not express Ang-2 mRNA. (D) The LoVo cell transfected with Ang-2 target shRNA exhibited markedly decreased expression levels of Ang-2 protein. Ang-2, Angiopoietin-2.
Figure 1. Ang-2 expression was investigated by reverse-transcription polymerase chain reaction and western blot analysis in colorectal cancer cell lines, HUVEC and normal cell line (CCD-841 CoN). (A) Among 5 colorectal cancer cell lines, 3 cell lines expressed Ang-2 mRNA, as did the HUVEC cell line. (B) LoVo cells expressed a markedly higher level of Ang-2 protein compared with other colorectal cancer cell lines, as detected by western blotting. (C) The LoVo cell line transfected with Ang-2 target shRNA did not express Ang-2 mRNA. (D) The LoVo cell transfected with Ang-2 target shRNA exhibited markedly decreased expression levels of Ang-2 protein. Ang-2, Angiopoietin-2.CC BY-NC-ND · PubMed Central
Figure 2. (A) The proliferation of LoVo cells following Ang-2-targeted shRNA transfection was significantly decreased compared with control LoVo cells and LoVo cell transfected with non-targeted shRNA. (B) The invasion ability of Ang-2 target shRNA transfected LoVo cells was decreased markedly compared with the control and non-target shRNA-transfected LoVo cells. ***P<0.05. Ang-2, Angiopoietin-2; shRNA, short hairpin RNA.
Figure 2. (A) The proliferation of LoVo cells following Ang-2-targeted shRNA transfection was significantly decreased compared with control LoVo cells and LoVo cell transfected with non-targeted shRNA. (B) The invasion ability of Ang-2 target shRNA transfected LoVo cells was decreased markedly compared with the control and non-target shRNA-transfected LoVo cells. ***P<0.05. Ang-2, Angiopoietin-2; shRNA, short hairpin RNA.CC BY-NC-ND · PubMed Central
Figure 3. The migration of Ang-2 target shRNA transfected LoVo cells was decreased compared with the control and non-target shRNA transfected LoVo cells. ***P<0.01. Ang-2, Angiopoietin-2; shRNA, short hairpin RNA.
Figure 3. The migration of Ang-2 target shRNA transfected LoVo cells was decreased compared with the control and non-target shRNA transfected LoVo cells. ***P<0.01. Ang-2, Angiopoietin-2; shRNA, short hairpin RNA.CC BY-NC-ND · PubMed Central
Figure 4. (A) Ang-2 was not expressed in N cells. However, Ang-2 expression was markedly increased in T cells. (B) The cumulative survival was significantly decreased in patients with high levels of Ang-2 compared with patients with low levels of Ang-2 expression, as determined by Kaplan Meier analysis (log rank test, P<0.001). T, colorectal cancer cells; N, normal colonic epithelial cells; Cum, cumulative; Ang-2, Angiopoietin-2.
Figure 4. (A) Ang-2 was not expressed in N cells. However, Ang-2 expression was markedly increased in T cells. (B) The cumulative survival was significantly decreased in patients with high levels of Ang-2 compared with patients with low levels of Ang-2 expression, as determined by Kaplan Meier analysis (log rank test, P<0.001). T, colorectal cancer cells; N, normal colonic epithelial cells; Cum, cumulative; Ang-2, Angiopoietin-2.CC BY-NC-ND · PubMed Central

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Citations

Cites

Research works printed but not linked: 28

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Metadata

Authors
Kim, H., Ahn, T. S., Kim, C.-J., Bae, S. B., Kim, H. J., Lee, C.-S., Kim, T. H., Im, J., Lee, S. H., Son, M. W., Lee, M. S., Baek, M. J., & Jeong, D.
Venue
Oncology Letters
Publisher
Spandidos Publications
Published
2017-05-18
Volume
14
Issue
1
Pages
553-560
ISSN
  • 1792-1074
  • 1792-1082
DOI
10.3892/ol.2017.6203
PMC
PMC5494651
Cited by
8
References
1
Language
en

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