Mangiferin regulates proliferation and apoptosis in glioma cells by induction of microRNA-15b and inhibition of MMP-9 expression
Open accessAbstract
Mangiferin, a flavonoid extracted from the leaves of the Anacardiaceae plant, the mango tree, has physiological activity and pharmacological effects in many aspects. The present study aimed to clarify the effect of mangiferin on proliferation and apoptosis of glioma cells and the mechanism of these curative effects of mangiferin. In this experiment, we detected the proliferation using 3-(4,5-dimethylthylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. Then, cell apoptosis of U87 glioma cells was measured with the Annexin V-FITC/propidium iodide (PI) apoptosis detection kit, DAPI staining assay and the caspase-3 and caspase-9 activity assay kit. Next, quantitative real-time PCR and gelatin zymography were used to analyze the expression of microRNA-15b (miR-15b) and matrix metalloproteinase-9 (MMP-9), respectively. MMP-9 agonist, miR-15b mimics and anti-miR-15b mimics were added to the U87 glioma cells for elucidating the mechanisms involved in the curative effects of mangiferin. In the present study, mangiferin notably restrained the proliferation and increased the apoptosis of the U87 glioma cells. Meanwhile, mangiferin specifically promoted the expression of miR-15b and suppressed the level of MMP-9 in the U87 glioma cells. miR-15b regulated the expression of MMP-9 in the U87 glioma cells. MMP-9 agonist and anti-miR‑15b reduced the curative effects of mangiferin in the U87 glioma cells. In summary, mangiferin regulates proliferation and apoptosis in glioma cells by induction of miR-15b and inhibition of MMP-9 expression.
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MGF exerts potent anticancer activity through multiple mechanisms that target key cellular processes underlying tumor initiation and progression.
“MGF exerts potent anticancer activity through multiple mechanisms that target key cellular processes underlying tumor initiation and progression.”
From Mangiferin in human disease: Multifaceted mechanisms and applications (Review) (2026) · cites this paper as [37]
The Discussion (page not specified) reports that mangiferin suppressed proliferation and induced apoptosis in U87 glioma cells, with changes involving miR-15b and MMP-9. This supports anticancer effects through several cellular processes, but the study does not establish potent activity across cancers or an effect on tumor initiation. (LLM, conf=0.82)
Experimental — accuracy not yet measuredIn glioma cells, MGF upregulates miR-15b, which induces apoptosis by downregulating WNT7A.
“In glioma cells, MGF upregulates miR-15b, which induces apoptosis by downregulating the expression of oncogenic targets such as WNT7A (37,64).”
From Mangiferin in human disease: Multifaceted mechanisms and applications (Review) (2026) · cites this paper as [37]
The Results section on miR-15b expression reports that mangiferin increased miR-15b in U87 glioma cells, and the anti-miR-15b results link that increase to apoptosis. The paper examines reduced MMP-9 expression, not downregulation of WNT7A; the WNT7A mechanism is unsupported by our copy. Page numbers are unavailable. (LLM, conf=0.90)
Experimental — accuracy not yet measuredMGF's anticancer mechanisms include induction of cell cycle arrest, promotion of apoptosis, inhibition of tumor invasion and metastasis, modulation of immune responses, and enhancement of radiosensitivity in glioblastoma.
“These include the induction of cell cycle arrest, the promotion of apoptosis through various intracellular pathways, the inhibition of tumor invasion and metastasis, the modulation of immune responses, and the enhancement of radiosensitivity in cancer such as glioblastoma (GBM) (36,37).”
From Mangiferin in human disease: Multifaceted mechanisms and applications (Review) (2026) · cites this paper as [37]
The Discussion (page unavailable) reports that mangiferin increased apoptosis and caspase activation in U87 glioma cells. The introductory passage also says mangiferin can arrest the cell cycle in a hepatoma cell line and has anti-inflammatory effects. This paper does not establish that mangiferin inhibits invasion or metastasis, modulates anticancer immune responses, or enhances radiosensitivity in glioblastoma; its U87 results concern proliferation, apoptosis, miR-15b and MMP-9. (LLM, conf=0.88)
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Cites
Pan, L.-L., Wang, A.-Y., Huang, Y.-Q., Luo, Y., & Ling, M. (2014) · Asian Pacific Journal of Cancer Prevention · doi:10.7314/apjcp.2014.15.17.7065 · Cited by 66 · see citing works
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Mangiferin: A natural miracle bioactive compound against lifestyle related disorders
Imran, M., Arshad, M. S., Butt, M. S., Kwon, J.-H., Arshad, M. U., & Sultan, M. T. (2017) · Lipids in Health and Disease · doi:10.1186/s12944-017-0449-y · Cited by 366 · see citing works
Mangiferin and Cancer: Mechanisms of Action
Gold-Smith, F., Fernandez, A., & Bishop, K. (2016) · Nutrients · doi:10.3390/nu8070396 · Cited by 158 · see citing works
Morozkina, S. N., Nhung Vu, T. H., Generalova, Y. E., Snetkov, P. P., & Uspenskaya, M. V. (2021) · Biomolecules · doi:10.3390/biom11010079 · Cited by 82 · see citing works
Recent Formulation Advances of Mangiferin
Barakat, S., Nasr, M., Ahmed, R. F., Badawy, S., & Mortada, N. (2022) · Revista Brasileira de Farmacognosia · doi:10.1007/s43450-022-00297-z · Cited by 28 · see citing works
Mangiferin Inhibits Human Lung Adenocarcinoma by Suppressing MiR-27b and MiR-92a
Chi, X.-J., Meng, J.-J., Lin, C.-Y., Su, Q.-S., Qin, Y.-Y., Wei, R.-H., Lan, D., & Huang, C. (2021) · Evidence-Based Complementary and Alternative Medicine · doi:10.1155/2021/2822950 · Cited by 10 · see citing works
Mangiferin in human disease: Multifaceted mechanisms and applications (Review)
Dai, Y., Huang, Q., Tan, M., Wang, Z., Jiang, C., Liu, Z., Zhang, S., & Song, S. (2026) · International Journal of Molecular Medicine · doi:10.3892/ijmm.2026.5736 · Cited by 3 · see citing works
- Authors
- XIAO, J., LIU, L., ZHONG, Z., XIAO, C., & ZHANG, J.
- Affiliations
- Zhongnan Hospital of Wuhan University (CN)
- Venue
- Oncology Reports
- Publisher
- Spandidos Publications
- Published
- 2015-04-21
- Volume
- 33
- Issue
- 6
- Pages
- 2815-2820
- ISSN
- 1021-335X (ISSN-L)
- 1791-2431
- OpenAlex
- W2167010616
- Cited by
- 51
- References
- 1
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 2 · 2023: 7 · 2022: 8 · 2021: 5 · 2020: 3 · 2019: 3 · 2018: 4 · 2017: 5 · 2016: 7 · 2015: 1
- FWCI
- 11.06
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Oncology Reports (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Mangiferin and Mango Extracts
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Pharmacology › Flavonoids in Medical Research
- Keywords
- mangiferin, glioma, MMP-9, cell proliferation, apoptosis, U87 cells, caspase-3, caspase-9, flavonoids, anticancer mechanism
- MeSH terms
- Brain Neoplasms, Cell Survival, Glioma, Humans, Neoplasm Invasiveness, Gene Expression Regulation, Neoplastic, Apoptosis, Matrix Metalloproteinase 9, MicroRNAs, Xanthones, Cell Line, Tumor, Cell Proliferation
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