Reduction of body weight by dietary garlic is associated with an increase in uncoupling protein mRNA expression and activation of AMP-activated protein kinase in diet-induced obese mice
Open accessAbstract
This study investigated the antiobesity effect of garlic in diet-induced obese mice. Male C57BL/6J mice were fed a high-fat diet (45% fat) for 8 wk to induce obesity. Subsequently, they were fed a high-fat control diet, high-fat diets supplemented with 2%, or 5% garlic (wt:wt) for another 7 wk. Dietary garlic reduced body weight and the mass of various white adipose tissue deposits and also ameliorated the high-fat diet-induced abnormal plasma and liver lipid profiles. Garlic supplementation significantly decreased the mRNA levels of adipogenic genes in white adipose tissues (WAT). However, consumption of garlic increased the expression of mRNA for uncoupling proteins in brown adipose tissue (BAT), liver, WAT, and skeletal muscle. Mice treated with garlic maintained a significantly higher body temperature than untreated mice during a 6-h, 4°C cold challenge and, notably, AMP-activated protein kinase (AMPK) activity was stimulated in BAT, liver, WAT, and skeletal muscle. These results suggest that the antiobesity effects of garlic were at least partially mediated via activation of AMPK, increased thermogenesis, and decreased expression of multiple genes involved in adipogenesis.
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Garlic extracts reduce body weight, adipose tissue mass and improve plasma lipid profiles in mice with high-fat diet-induced obesity, mediated by downregulation of multiple genes involved in adipogenesis and upregulation of mitochondrial inner membrane proteins.
“Garlic extracts have been reported for their activity in reducing body weight, adipose tissue mass and improved plasma lipid profiles in mice with high-fat diet-induced obesity and these effects mediated by the downregulation of multiple genes expression that is included in adipogenesis along with upregulation of the mitochondrial inner membrane proteins expression [116].”
From Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review (2020) · cites this paper as [116]
The antiobesity effect of garlic extracts is attributed to stimulation of AMP-activated protein kinase, increased thermogenesis, and decreased multiple genes expression involved in adipogenesis.
“Lee et al. [116] revealed that the antiobesity effect of garlic extracts attributed to stimulation of AMP-activated protein kinase (AMPK) as well as increased thermogenesis and decreased multiple genes expression that is included in adipogenesis.”
From Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review (2020) · cites this paper as [116]
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“AMPK activity was evaluated using an AMPK Kinase Assay kit as described previously [28].”
MethodSelf-citation
- Authors
- Lee, M.-S., Kim, I.-H., Kim, C.-T., & Kim, Y.
- Affiliations
- Ewha Womans University (KR)
- Korea University (KR)
- Korea Food Research Institute (KR)
- Corresponding author
- Kim, Yangha
- Venue
- The Journal of Nutrition
- Publisher
- Elsevier BV
- Published
- 2011-11
- Volume
- 141
- Issue
- 11
- Pages
- 1947-1953
- ISSN
- 0022-3166 (ISSN-L)
- OpenAlex
- W2145402048
- Cited by
- 100
- References
- 49
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 4 · 2023: 8 · 2022: 9 · 2021: 9 · 2020: 5 · 2019: 7 · 2018: 9 · 2017: 4 · 2016: 4 · 2015: 8 · 2014: 8 · 2013: 6 · 2012: 6
- FWCI
- 7.69
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation
- National Research Foundation of Korea
- Korea Food Research Institute (202007-03-03-WT011)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Garlic and Onion Studies
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- garlic, anti-obesity, diet-induced obesity, uncoupling proteins, AMPK, brown adipose tissue, white adipose tissue, adipogenesis, thermogenesis, high-fat diet, lipid profile, weight loss, cold tolerance
- MeSH terms
- Adipose Tissue, Animals, Body Weight, Diet, Enzyme Activation, Garlic, Male, Mice, Inbred C57BL, Obesity, RNA, Messenger, Mitochondrial Proteins, Mice, AMP-Activated Protein Kinases, Real-Time Polymerase Chain Reaction
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