L-Arginine Supplementation Prevents Increases in Intestinal Permeability and Bacterial Translocation in Male Swiss Mice Subjected to Physical Exercise under Environmental Heat Stress
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Dietary supplementation with l-arginine has been shown to improve the intestinal barrier in many experimental models. This study investigated the effects of arginine supplementation on the intestinal permeability and bacterial translocation (BT) induced by prolonged physical exercise under heat stress. Under anesthesia, male Swiss mice (5-wk-old) were implanted with an abdominal sensor to record their core body temperature (T(core)). After recovering from surgery, the mice were divided into 3 groups: a non-supplemented group that was fed the standard diet formulated by the American Institute of Nutrition (AIN-93G; control), a non-supplemented group that was fed the AIN-93G diet and subjected to exertional hyperthermia (H-NS), and a group supplemented with l-arginine at 2% and subjected to exertional hyperthermia (H-Arg). After 7 d of treatment, the H-NS and H-Arg mice were forced to run on a treadmill (60 min, 8 m/min) in a warm environment (34°C). The control mice remained at 24°C. Thirty min before the exercise or control trials, the mice received a diethylenetriamine pentaacetic acid (DTPA) solution labeled with technetium-99m ((99m)Tc-DTPA) or (99m)Tc-Escherichia coli by gavage to assess intestinal permeability and BT, respectively. The H-NS mice terminated the exercise with T(core) values of ∼40°C, and, 4 h later, presented a 12-fold increase in the blood uptake of (99m)Tc-DTPA and higher bacterial contents in the blood and liver than the control mice. Although supplementation with arginine did not change the exercise-induced increase in T(core), it prevented the increases in intestinal permeability and BT caused by exertional hyperthermia. Our results indicate that dietary l-arginine supplementation preserves the integrity of the intestinal epithelium during exercise under heat stress, acting through mechanisms that are independent of T(core) regulation.
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The composition of the gut microbiota, metabolic interactions between microbial species, and the amount and type of main dietary macro- and micronutrients determine the types and amount of SCFAs produced by gut microorganisms.
“The composition of the gut microbiota, metabolic interactions between microbial species [52] and the amount and type of the main dietary macro- and micronutrients determine the types and amount of SCFAs produced by gut microorganisms [75, 76].”
From Exercise-induced stress behavior, gut-microbiota-brain axis and diet: a systematic review for athletes (2016) · cites this paper as [76]
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“The composition of the gut microbiota, metabolic interactions between microbial species [52] and the amount and type of the main dietary macroand micronutrients determine the types and amount of SCFAs produced by gut microorganisms [75, 76].”
BackgroundMenni, C., Zierer, J., Pallister, T., Jackson, M. A., Long, T., Mohney, R. P., Steves, C. J., Spector, T. D., & Valdes, A. M. (2017) · Scientific Reports · doi:10.1038/s41598-017-10382-2 · Cited by 237 · see citing works
- Authors
- Costa, K. A., Soares, A. D. N., Wanner, S. P., Santos, R. D. G. C. D., Fernandes, S. O. A., Martins, F. D. S., Nicoli, J. R., Coimbra, C. C., & Cardoso, V. N.
- Affiliations
- Universidade Federal de Minas Gerais (BR)
- Corresponding author
- Cardoso, Valbert Nascimento
- ORCID
- Venue
- The Journal of Nutrition
- Publisher
- Elsevier BV
- Published
- 2014-02
- Volume
- 144
- Issue
- 2
- Pages
- 218-223
- ISSN
- 0022-3166 (ISSN-L)
- OpenAlex
- W2037896325
- Cited by
- 84
- References
- 52
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 11 · 2023: 9 · 2022: 5 · 2021: 8 · 2020: 8 · 2019: 6 · 2018: 6 · 2017: 5 · 2016: 7 · 2015: 7 · 2014: 5
- FWCI
- 4.40
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES/Brazil)
- Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
- Conselho Nacional de Pesquisa (CNPq/Brazil)
- Pró-Reitoria de Pesquisa da Universidade Federal de Minas Gerais (PRPq/UFMG)
- Topics
- Health Sciences › Medicine › Physiology › Thermoregulation and physiological responses
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Nursing › Nutrition and Dietetics › Clinical Nutrition and Gastroenterology
- Keywords
- arginine supplementation, intestinal permeability, bacterial translocation, heat stress, physical activity, intestinal barrier, core body temperature, Tc-99m DTPA
- MeSH terms
- Animals, Arginine, Body Temperature, Pentetic Acid, Escherichia coli, Fever, Hot Temperature, Intestinal Mucosa, Intestines, Liver, Male, Mice, Inbred Strains, Permeability, Physical Conditioning, Animal, Running, Stress, Physiological, Bacterial Translocation, Dietary Supplements, Mice
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