Intra- and Intercompartmental Movement of γδ T Cells: Intestinal Intraepithelial and Peripheral γδ T Cells Represent Exclusive Nonoverlapping Populations with Distinct Migration Characteristics
Abstract
Unlike the ∼1% of γδ TCR-positive T cells being regularly present in blood and secondary lymphoid organs (peripheral γδ T cells), ∼50–60% of small intestinal intraepithelial lymphocytes (iIELs) in the mouse express the γδ TCR (γδ iIELs). In this study, we investigated the overlap and exchange of γδ iIELs and γδ T cells found in peripheral secondary lymphoid organs. Using two-photon laser-scanning microscopy, we found γδ T cells within peripheral lymph nodes to be highly motile, whereas γδ iIELs were characterized by a locally confined scanning behavior. Our results implied a strict separation of peripheral γδ T cells and γδ iIELs. Nevertheless, γδ iIELs could be efficiently regenerated from bone marrow-derived precursors in irradiated or T cell-deficient adult mice. However, outside the intestinal epithelium, survival of γδ iIELs was very poor. In CCR9-deficient mice, homing of γδ iIELs was impaired, but did not lead to an accumulation of γδ iIEL-like cells in the periphery. Conversely, in situations in which specific γδ iIEL niches were empty, adoptive transfer of isolated γδ iIELs led to a sustained engraftment of transferred γδ iIELs in the intestinal epithelium for at least 100 d. Furthermore, we demonstrated by heterotopic intestinal transplantation experiments that an exchange of γδ iIELs only rarely happens in the steady state of adult mice. We therefore conclude that peripheral versus intestinal intraepithelial γδ T cells are exclusive, nonoverlapping populations that virtually do not exchange with each other.
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Acquisition of surface markers during development such as CD103 (αE integrin), α4β7 and CCR9 ensure homing and tissue residency of IELs.
“Moreover, acquisition of surface markers during development such as CD103 (αE integrin), α4β7 and CCR9 ensure homing and tissue residency of IELs [324] (see Table 15).”
From Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) (2021) · cites this paper as [324]
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- Authors
- Chennupati, V., Worbs, T., Liu, X., Malinarich, F. H., Schmitz, S., Haas, J. D., Malissen, B., Förster, R., & Prinz, I.
- Affiliations
- Medizinische Hochschule Hannover (DE)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Chennupati, Vijaykumar
- Worbs, Tim
- Liu, Xiaosun
- Schmitz, Susanne
- Prinz, Immo
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-11-01
- Volume
- 185
- Issue
- 9
- Pages
- 5160-5168
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W2101126186
- Cited by
- 84
- References
- 63
- Citations by year
- 2026: 6 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 5 · 2021: 5 · 2020: 7 · 2019: 3 · 2018: 6 · 2017: 9 · 2016: 5 · 2015: 7 · 2014: 6 · 2013: 6 · 2012: 9
- FWCI
- 2.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- HAL AMU (submittedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Keywords
- γδ T cells, intraepithelial lymphocytes, intestinal intraepithelial lymphocytes, lymph node homing, CCR9, adoptive transfer, two-photon imaging
- MeSH terms
- Animals, Cell Movement, Cell Separation, Flow Cytometry, Intestinal Mucosa, Lymph Nodes, Mice, Inbred C57BL, T-Lymphocytes, T-Lymphocyte Subsets, Receptors, Antigen, T-Cell, gamma-delta, Mice, Knockout, Microscopy, Confocal, Immunity, Mucosal, Cell Lineage, Adoptive Transfer, Mice
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