The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Endogenous IL-33 Is Highly Expressed in Mouse Epithelial Barrier Tissues, Lymphoid Organs, Brain, Embryos, and Inflamed Tissues: In Situ Analysis Using a Novel Il-33–LacZ Gene Trap Reporter Strain
Abstract
IL-33 (previously known as NF from high endothelial venules) is an IL-1 family cytokine that signals through the ST2 receptor and drives cytokine production in mast cells, basophils, eosinophils, invariant NKT and NK cells, Th2 lymphocytes, and type 2 innate immune cells (natural helper cells, nuocytes, and innate helper 2 cells). Little is known about endogenous IL-33; for instance, the cellular sources of IL-33 in mouse tissues have not yet been defined. In this study, we generated an Il-33–LacZ gene trap reporter strain (Il-33Gt/Gt) and used this novel tool to analyze expression of endogenous IL-33 in vivo. We found that the Il-33 promoter exhibits constitutive activity in mouse lymphoid organs, epithelial barrier tissues, brain, and embryos. Immunostaining with anti–IL-33 Abs, using Il-33Gt/Gt (Il-33–deficient) mice as control, revealed that endogenous IL-33 protein is highly expressed in mouse epithelial barrier tissues, including stratified squamous epithelia from vagina and skin, as well as cuboidal epithelium from lung, stomach, and salivary gland. Constitutive expression of IL-33 was not detected in blood vessels, revealing the existence of species-specific differences between humans and mice. Importantly, IL-33 protein was always localized in the nucleus of producing cells with no evidence for cytoplasmic localization. Finally, strong expression of the Il-33–LacZ reporter was also observed in inflamed tissues, in the liver during LPS-induced endotoxin shock, and in the lung alveoli during papain-induced allergic airway inflammation. Together, our findings support the possibility that IL-33 may function as a nuclear alarmin to alert the innate immune system after injury or infection in epithelial barrier tissues.
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Cites
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Nuocytes represent a new innate effector leukocyte that mediates type-2 immunity
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Self-citationSystemically dispersed innate IL-13–expressing cells in type 2 immunity
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IL-33 and ST2 comprise a critical biomechanically induced and cardioprotective signaling system
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Cited by
IL-33: an alarmin cytokine with crucial roles in innate immunity, inflammation and allergy
Cayrol, C., & Girard, J.-P. (2014) · Current Opinion in Immunology · doi:10.1016/j.coi.2014.09.004 · Cited by 699
Self-citationVainchtein, I. D., Chin, G., Cho, F. S., Kelley, K. W., Miller, J. G., Chien, E. C., Liddelow, S. A., Nguyen, P. T., Nakao-Inoue, H., Dorman, L. C., et al. (2018) · Science · doi:10.1126/science.aal3589 · Cited by 657
Interleukin-33 in tissue homeostasis, injury, and inflammation
Molofsky, A. B., Savage, A. K., & Locksley, R. M. (2015) · Immunity · doi:10.1016/j.immuni.2015.06.006 · Cited by 638
The macrophage switch in obesity development
Castoldi, A., Naffah de Souza, C., Câmara, N. O. S., & Moraes-Vieira, P. M. (2016) · Frontiers in Immunology · doi:10.3389/fimmu.2015.00637 · Cited by 575
Panduro, M., Benoist, C., & Mathis, D. (2016) · Annual Review of Immunology · doi:10.1146/annurev-immunol-032712-095948 · Cited by 536
Metadata
- Authors
- Pichery, M., Mirey, E., Mercier, P., Lefrancais, E., Dujardin, A., Ortega, N., & Girard, J.-P.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-04-01
- Volume
- 188
- Issue
- 7
- Pages
- 3488-3495
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W2116135404
- Cited by
- 466
- References
- 44
- FWCI
- 10.60
- Type
- journal article
- Language
- en
- Open access
- closed
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- Pichery, M., Mirey, E., Mercier, P., Lefrancais, E., Dujardin, A., Ortega, N., & Girard, J.-P. (2012). Endogenous IL-33 Is Highly Expressed in Mouse Epithelial Barrier Tissues, Lymphoid Organs, Brain, Embryos, and Inflamed Tissues: In Situ Analysis Using a Novel Il-33–LacZ Gene Trap Reporter Strain. The Journal of Immunology. https://doi.org/10.4049/jimmunol.1101977
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