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MicroRNA let-7c Regulates Macrophage Polarization
Open accessAbstract
Macrophages demonstrate a high level of plasticity, with the ability to undergo dynamic transition between M1 and M2 polarized phenotypes. The role of microRNAs (miRNAs) in regulating macrophage polarization has been largely undefined. In this study, we found that miRNA let-7c is expressed at a higher level in M-BMM (M2 macrophages) than in GM-BMM (M1 macrophages). let-7c levels are also greater in alveolar macrophages from fibrotic lungs as compared with those from normal lungs. let-7c expression was decreased when M-BMM converted to GM-BMM, whereas it increased when GM-BMM converted to M-BMM. LPS stimulation reduced let-7c expression in M-BMM. We found that overexpression of let-7c in GM-BMM diminished M1 phenotype expression while promoting polarization to the M2 phenotype. In contrast, knockdown of let-7c in M-BMM promoted M1 polarization and diminished M2 phenotype expression. We found that let-7c targets C/EBP-δ, a transcriptional factor that plays an important role in inflammatory response. Furthermore, we found that let-7c regulates bactericidal and phagocytic activities of macrophages, two functional phenotypes implicated in macrophage polarization. Our data suggest that the miRNA let-7c plays an important role in regulating macrophage polarization.
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“Expression of let-7c is suppressed by LPS, whereas its upregulation results in a change from the M1 to M2 phenotype by targeting the C/EBP-δ transcription factor [110].”
From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [110]
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“Overexpression of let-7c, by promoting the M2 phenotype, increases phagocytosis of apoptotic cells, whereas its knockdown decreases it [110].”
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Citations
Cites
Exploring the full spectrum of macrophage activation
Mosser, D. M., & Edwards, J. P. (2008) · Nature Reviews Immunology · doi:10.1038/nri2448 · Cited by 9,144
Monocyte and macrophage heterogeneity
Gordon, S., & Taylor, P. R. (2005) · Nature Reviews Immunology · doi:10.1038/nri1733 · Cited by 5,190
The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans
Reinhart, B. J., Slack, F. J., Basson, M., Pasquinelli, A. E., Bettinger, J. C., Rougvie, A. E., Horvitz, H. R., & Ruvkun, G. (2000) · Nature · doi:10.1038/35002607 · Cited by 4,989
Development of Monocytes, Macrophages, and Dendritic Cells
Geissmann, F., Manz, M. G., Jung, S., Sieweke, M. H., Merad, M., & Ley, K. (2010) · Science · doi:10.1126/science.1178331 · Cited by 3,049
Causes and consequences of microRNA dysregulation in cancer
Croce, C. M. (2009) · Nature Reviews Genetics · doi:10.1038/nrg2634 · Cited by 3,039
Martínez, F. O., Gordon, S., Locati, M., & Mantovani, A. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.177.10.7303 · Cited by 2,399
MicroRNA-21 contributes to myocardial disease by stimulating MAP kinase signalling in fibroblasts
Thum, T., Gross, C., Fiedler, J., Fischer, T., Kissler, S., Bussen, M., Galuppo, P., Just, S., Rottbauer, W., Frantz, S., et al. (2008) · Nature · doi:10.1038/nature07511 · Cited by 2,349
Transcriptional regulation of macrophage polarization: enabling diversity with identity
Lawrence, T., & Natoli, G. (2011) · Nature Reviews Immunology · doi:10.1038/nri3088 · Cited by 2,284
Grishok, A., Pasquinelli, A. E., Conte, D., Li, N., Parrish, S., Ha, I., Baillie, D. L., Fire, A., Ruvkun, G., & Mello, C. C. (2001) · Cell · doi:10.1016/s0092-8674(01)00431-7 · Cited by 1,908
Small non-coding RNAs in animal development
Stefani, G., & Slack, F. J. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2347 · Cited by 1,387
Iliopoulos, D., Hirsch, H. A., & Struhl, K. (2009) · Cell · doi:10.1016/j.cell.2009.10.014 · Cited by 1,374
IRF5 promotes inflammatory macrophage polarization and TH1-TH17 responses
Krausgruber, T., Blazek, K. D., Smallie, T., Alzabin, S., Lockstone, H. E., Sahgal, N., Hussell, T., Feldmann, M., & Udalova, I. A. (2011) · Nature Immunology · doi:10.1038/ni.1990 · Cited by 1,341
Satoh, T., Takeuchi, O., Vandenbon, A., Yasuda, K., Tanaka, Y., Kumagai, Y., Miyake, T., Matsushita, K., Okazaki, T., Saitoh, T., et al. (2010) · Nature Immunology · doi:10.1038/ni.1920 · Cited by 1,156
Verreck, F. A. W., de Boer, T., Langenberg, D. M. L., Hoeve, M. A., Kramer, M., Vaisberg, E., Kastelein, R., Kolk, A. H. J., Malefyt, R. D. W., & Ottenhoff, T. H. M. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0400983101 · Cited by 969
Differential macrophage programming in the tumor microenvironment
Ruffell, B., Affara, N. I., & Coussens, L. M. (2012) · Trends in Immunology · doi:10.1016/j.it.2011.12.001 · Cited by 878
Ponomarev, E. D., Veremeyko, T., Barteneva, N., Krichevsky, A. M., & Weiner, H. L. (2011) · Nature Medicine · doi:10.1038/nm.2266 · Cited by 787
Ruffell, D., Mourkioti, F., Gambardella, A., Kirstetter, P., Lopez, R. G., Rosenthal, N., & Nerlov, C. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0908641106 · Cited by 626
The interleukin‐1 receptor/Toll‐like receptor superfamily: 10 years of progress
O'Neill, L. A. J. (2008) · Immunological Reviews · doi:10.1111/j.1600-065x.2008.00701.x · Cited by 623
let-7 microRNAs in development, stem cells and cancer
Büssing, I., Slack, F. J., & Großhans, H. (2008) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2008.07.001 · Cited by 615
How Toll-like receptors signal: what we know and what we don’t know
O'Neill, L. A. J. (2005) · Current Opinion in Immunology · doi:10.1016/j.coi.2005.11.012 · Cited by 614
Fleetwood, A. J., Lawrence, T., Hamilton, J. A., & Cook, A. D. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.178.8.5245 · Cited by 607
The Kinase Akt1 Controls Macrophage Response to Lipopolysaccharide by Regulating MicroRNAs
Androulidaki, A., Iliopoulos, D., Arranz, A., Doxaki, C., Schworer, S., Zacharioudaki, V., Margioris, A. N., Tsichlis, P. N., & Tsatsanis, C. (2009) · Immunity · doi:10.1016/j.immuni.2009.06.024 · Cited by 581
Obstacles and opportunities for understanding macrophage polarization
Murray, P. J., & Wynn, T. A. (2011) · Journal of Leukocyte Biology · doi:10.1189/jlb.0710409 · Cited by 581
Defining GM-CSF– and Macrophage-CSF–Dependent Macrophage Responses by In Vitro Models
Lacey, D. C., Achuthan, A. A., Fleetwood, A. J., Dinh, H., Roiniotis, J., Scholz, G. M., Chang, M. W., Beckman, S. K., Cook, A. D., & Hamilton, J. A. (2012) · The Journal of Immunology · doi:10.4049/jimmunol.1103426 · Cited by 528
Epigenetic regulation of the alternatively activated macrophage phenotype
Ishii, M., Wen, H., Corsa, C. A. S., Liu, T., Coelho, A. L., Allen, R., Carson, W. F., Cavassani, K. A., Li, X., Lukacs, N. W., et al. (2009) · Blood · doi:10.1182/blood-2009-04-217620 · Cited by 505
A Vicious Circle of Alveolar Macrophages and Fibroblasts Perpetuates Pulmonary Fibrosis via CCL18
Prasse, A., Pechkovsky, D. V., Toews, G. B., Jungraithmayr, W., Kollert, F., Goldmann, T., Vollmer, E., Müller-Quernheim, J., & Zissel, G. (2006) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200509-1518oc · Cited by 497
Chen, X., Splinter, P. L., O’Hara, S. P., & LaRusso, N. F. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m702633200 · Cited by 490
Ly6Chi monocytes direct alternatively activated profibrotic macrophage regulation of lung fibrosis
Gibbons, M. A., MacKinnon, A. C., Ramachandran, P., Dhaliwal, K., Duffin, R., Phythian-Adams, A. T., van Rooijen, N., Haslett, C., Howie, S. E., Simpson, A. J., et al. (2011) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201010-1719oc · Cited by 466
Zhuang, G., Meng, C., Guo, X., Cheruku, P. S., Shi, L., Xu, H., Li, H., Wang, G., Evans, A. R., Safe, S., et al. (2012) · Circulation · doi:10.1161/circulationaha.111.087817 · Cited by 428
Fadok, V. A., Warner, M. L., Bratton, D. L., & Henson, P. M. (1998) · PubMed · Cited by 406
Puig‐Kröger, A., Sierra‐Filardi, E., Domínguez‐Soto, Á., Samaniego, R., Corcuera, M. T., Gómez, F., Ratnam, M., Sánchez‐Mateos, P., & Corbí, A. L. (2009) · Cancer Research · doi:10.1158/0008-5472.can-09-2050 · Cited by 396
Identifying Functional MicroRNAs in Macrophages with Polarized Phenotypes
Graff, J. W., Dickson, A. M., Clay, G., McCaffrey, A. P., & Wilson, M. E. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.327031 · Cited by 367
Fadok, V. A., Warner, M. L., Bratton, D. L., & Henson, P. M. (1998) · The Journal of Immunology · doi:10.4049/jimmunol.161.11.6250 · Cited by 353
Resolution-phase macrophages possess a unique inflammatory phenotype that is controlled by cAMP
Byström, J., Evans, I. M., Newson, J., Stables, M. J., Toor, I. S., van Rooijen, N., Crawford, M., Colville‐Nash, P., Farrow, S. N., & Gilroy, D. W. (2008) · Blood · doi:10.1182/blood-2007-12-129767 · Cited by 350
MicroRNA-125b Potentiates Macrophage Activation
Chaudhuri, A. A., So, A. Y.-L., Sinha, N., Gibson, W. S. J., Taganov, K. D., O’Connell, R. M., & Baltimore, D. (2011) · The Journal of Immunology · doi:10.4049/jimmunol.1102001 · Cited by 327
MicroRNAs and cardiac pathology
Latronico, M. V. G., & Condorelli, G. (2009) · Nature Reviews Cardiology · doi:10.1038/nrcardio.2009.56 · Cited by 320
Schulte, L. N., Eulálio, A., Mollenkopf, H., Reinhardt, R., & Vogel, J. (2011) · The EMBO Journal · doi:10.1038/emboj.2011.94 · Cited by 316
Histone deacetylase 3 is an epigenomic brake in macrophage alternative activation
Mullican, S. E., Gaddis, C. A., Alenghat, T., Nair, M. G., Giacomin, P. R., Everett, L. J., Feng, D., Steger, D. J., Schug, J., Artis, D., et al. (2011) · Genes & Development · doi:10.1101/gad.175950.111 · Cited by 314
Fleetwood, A. J., Dinh, H., Cook, A. D., Hertzog, P. J., & Hamilton, J. A. (2009) · Journal of Leukocyte Biology · doi:10.1189/jlb.1108702 · Cited by 312
Litvak, V., Ramsey, S. A., Rust, A. G., Zak, D. E., Kennedy, K. A., Lampano, A. E., Nykter, M., Shmulevich, I., & Aderem, A. (2009) · Nature Immunology · doi:10.1038/ni.1721 · Cited by 295
Participation of miR-200 in Pulmonary Fibrosis
Yang, S., Banerjee, S., de Freitas, A., Sanders, Y. Y., Ding, Q., Matalon, S., Thannickal, V. J., Abraham, E. H., & Liu, G. (2011) · American Journal Of Pathology · doi:10.1016/j.ajpath.2011.10.005 · Cited by 258
Self-citationMicroRNAs in Immune Response and Macrophage Polarization
Liu, G., & Abraham, E. H. (2013) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.112.300068 · Cited by 248
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Uderhardt, S., i, M. H., Oskolkova, O., Aschermann, S., Bicker, W., Ipseiz, N., Sarter, K., Frey, B., Rothe, T., Voll, R. E., et al. (2012) · Immunity · doi:10.1016/j.immuni.2012.03.010 · Cited by 246
Proinflammatory Role for let-7 MicroRNAS in Experimental Asthma
Polikepahad, S., Knight, J. M., Naghavi, A. O., Oplt, T., Creighton, C. J., Shaw, C. A., Benham, A. L., Kim, J. K., Soibam, B., Harris, R. A., et al. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.145698 · Cited by 242
Pottelberge, G. R. V., Mestdagh, P., Bracke, K. R., Thas, O., van Durme, Y. M. T. A., Joos, G., Vandesompele, J., & Brusselle, G. (2010) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201002-0304oc · Cited by 236
Lung environment determines unique phenotype of alveolar macrophages
Guth, A., Janssen, W. J., Bosio, C. M., Crouch, E. C., Henson, P. M., & DOW, S. W. (2009) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.90625.2008 · Cited by 213
Sierra‐Filardi, E., Vega, M. A., Sánchez‐Mateos, P., Corbí, A. L., & Puig‐Kröger, A. (2010) · Immunobiology · doi:10.1016/j.imbio.2010.05.020 · Cited by 209
Baran, C. P., Opalek, J. M., McMaken, S., Newland, C. A., O’Brien, J. M., Hunter, M. G., Bringardner, B. D., Monick, M. M., Brigstock, D. R., Stromberg, P. C., et al. (2007) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200609-1279oc · Cited by 196
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Pandey, A. K., Agarwal, P., Kaur, K., & Datta, M. (2009) · Cellular Physiology and Biochemistry · doi:10.1159/000218169 · Cited by 183
Park, S., Jung, M.-Y., Lee, S.-J., Kang, K.-B., Gratchev, A. N., Riabov, V., Kzhyshkowska, J. G., & Kim, I. (2009) · Journal of Cell Science · doi:10.1242/jcs.049569 · Cited by 163
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Jakubzick, C., Choi, E. S., Joshi, B. H., Keane, M. P., Kunkel, S. L., Puri, R. K., & Hogaboam, C. M. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.171.5.2684 · Cited by 158
Hu, G., Zhou, R., Liu, J., Gong, A., Eischeid, A. N., Dittman, J. W., & Chen, X. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.0804362 · Cited by 119
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Takeuch, O., & Akira, S. (2011) · European Journal of Immunology · doi:10.1002/eji.201141792 · Cited by 107
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Lu, Y., Kim, I., Lye, E., Shen, F., Suzuki, N., Suzuki, S., Gerondakis, S., Akira, S., Gaffen, S. L., Yeh, W., et al. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.0802971 · Cited by 106
Hou, W., Tian, Q., Steuerwald, N., Schrum, L. W., & Bonkovsky, H. L. (2012) · Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms · doi:10.1016/j.bbagrm.2012.06.001 · Cited by 103
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Maitra, U., Gan, L., Chang, S., & Li, L. (2011) · The Journal of Immunology · doi:10.4049/jimmunol.1003300 · Cited by 82
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Zhou, D., Huang, C., Lin, Z., Zhan, S., Kong, L., Fang, C., & Li, J. (2014) · Cellular Signalling · doi:10.1016/j.cellsig.2013.11.004 · Cited by 778
Metadata
- Authors
- Banerjee, S., Xie, N., Cui, H., Tan, Z., Yang, S., Icyuz, M., Abraham, E., & Liu, G.
- Affiliations
- University of Alabama at Birmingham (US)
- Wake Forest University Health Sciences (US)
- Corresponding author
- Banerjee, Sami
- Xie, Na
- Cui, Huachun
- Tan, Zheng
- Yang, Shanzhong
- Icyuz, Mert
- Liu, Gang
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2013-06
- Volume
- 190
- Issue
- 12
- Pages
- 6542-6549
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- PMC
- PMC3679284
- OpenAlex
- W1927296860
- Cited by
- 320
- References
- 62
- Citations by year
- 2026: 5 · 2025: 18 · 2024: 24 · 2023: 20 · 2022: 28 · 2021: 33 · 2020: 26 · 2019: 26 · 2018: 31 · 2017: 22 · 2016: 21 · 2015: 29 · 2014: 17 · 2013: 7
- FWCI
- 13.65
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Heart, Lung, and Blood Institute (HL 076206, R21HL097218, HL-105473, R01HL105473, R01HL076206, HL-097218)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (P30AR048311)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Immunology and Microbiology › Immunology › Macrophage Migration Inhibitory Factor
- Keywords
- let-7c microRNA, macrophage polarization, M1/M2 macrophages, C/EBPδ, alveolar macrophages, pulmonary fibrosis, lipopolysaccharide, phagocytosis, bactericidal activity, bone marrow-derived macrophages
- MeSH terms
- Animals, Cell Differentiation, Flow Cytometry, Macrophages, Mice, Inbred BALB C, Mice, Inbred C57BL, Phenotype, Transfection, Blotting, Western, Cell Polarity, Reverse Transcriptase Polymerase Chain Reaction, MicroRNAs, Mice, Real-Time Polymerase Chain Reaction
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