CoChat
Sign Up
Cited by 42Asthma and respiratory diseasesPeroxisome Proliferator-Activated ReceptorsChronic Obstructive Pulmonary Disease (COPD) Research

The nitrated fatty acid 10-nitro-oleate attenuates allergic airway disease

Reddy, Aravind T, Lakshmi, Sowmya P, Dornadula, Sireesh, Pinni, Sudheer, Rampa, Dileep R, Reddy, Raju C·2013·The Journal of Immunology
DOI 10.4049/jimmunol.1300730
Open this paper in CoChat — free account

Abstract

Asthma is a serious, growing problem worldwide. Inhaled steroids, the current standard therapy, are not always effective in this chronic inflammatory disease and can cause adverse effects. We tested the hypothesis that nitrated fatty acids (NFAs) may provide an effective alternative treatment. NFAs are endogenously produced by nonenzymatic reaction of NO with unsaturated fatty acids and exert anti-inflammatory actions both by activating the nuclear hormone receptor peroxisome proliferator-activated receptor (PPAR)γ and via PPAR-independent mechanisms, but whether they might ameliorate allergic airway disease was previously untested. We found that pulmonary delivery of the NFA 10-nitro-oleic acid (OA-NO2) reduced the severity of murine allergic airway disease, as assessed by various pathological and molecular markers. Fluticasone, an inhaled steroid commonly used to treat asthma, produced similar effects on most end points, but only OA-NO2 induced robust apoptosis of neutrophils and their phagocytosis by alveolar macrophages. This suggests that OA-NO2 may be particularly effective in neutrophil-rich, steroid-resistant severe asthma. In primary human bronchial epithelial cells, OA-NO2 blocked phosphorylation and degradation of IκB and enhanced inhibitory binding of PPARγ to NF-κB. Our results indicate that the NFA OA-NO2 is efficacious in preclinical models of allergic airway disease and may have potential for treating asthma patients.

Integrity signals

Registry

Crossref · 10.4049/jimmunol.1300730

Cites

47 of 47 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • NO2-FA display signaling actions that expand both the pharmacokinetics and scope of nitric oxide (·NO) signaling events to include actions beyond the activation of guanylate cyclase-dependent cGMP production.

    “NO2-FA display signaling actions that expands both the pharmacokinetics and scope of nitric oxide (·NO) signaling events to include actions beyond the activation of guanylate cyclase-dependent cGMP production [4–7].”

    From Nitrite and nitrate-dependent generation of anti-inflammatory fatty acid nitroalkenes (2015) · cites this paper as [7]

Cites

Flagged first, then by citations

1 of the 48 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Related

Authors
Reddy, A. T., Lakshmi, S. P., Dornadula, S., Pinni, S., Rampa, D. R., & Reddy, R. C.
Affiliations
University of Pittsburgh (US)
Corresponding author
Reddy, Raju C
ORCID
Venue
The Journal of Immunology
Publisher
Oxford University Press (OUP)
Published
2013-09-01
Volume
191
Issue
5
Pages
2053-2063
ISSN
  • 0022-1767 (ISSN-L)
  • 1550-6606
DOI
10.4049/jimmunol.1300730
OpenAlex
W1909650275
Cited by
42
References
48
Citations by year
2025: 1 · 2024: 1 · 2023: 4 · 2022: 3 · 2021: 2 · 2020: 1 · 2019: 8 · 2018: 6 · 2016: 6 · 2015: 7 · 2014: 3
FWCI
3.70
Type
journal article
Language
en
Open access
closed
Licence
academic.oup.com/pages/standard-publication-reuse-rights (vor)
Locations
  • The Journal of Immunology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Heart, Lung, and Blood Institute (HL-093196, R01HL093196)
Topics
  • Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
Keywords
nitrated fatty acids, allergic airway disease, asthma, PPARγ, NF-κB, neutrophil apoptosis, alveolar macrophages, fluticasone, steroid resistance, bronchial epithelial cells, anti-inflammatory therapy
MeSH terms
Animals, Anti-Inflammatory Agents, Asthma, Bronchoalveolar Lavage Fluid, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Fatty Acids, Fluorescent Antibody Technique, Humans, Male, Mice, Inbred C57BL, Oleic Acids, Blotting, Western, Microscopy, Confocal, Reverse Transcriptase Polymerase Chain Reaction, Immunoprecipitation, Mice, Real-Time Polymerase Chain Reaction

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review