Red cell membrane and plasma linoleic acid nitration products: Synthesis, clinical identification, and quantitation
Open accessAbstract
Nitric oxide ( NO) and its reactive metabolites mediate the oxidation, nitration, and nitrosation of DNA bases, amino acids, and lipids. Here, we report the structural characterization and quantitation of two allylic nitro derivatives of linoleic acid (LNO 2 ), present as both free and esterified species in human red cell membranes and plasma lipids. The LNO 2 isomers 10-nitro-9- cis , 12- cis -octadecadienoic acid and 12-nitro-9- cis , 12- cis -octadecadienoic acid were synthesized and compared with red cell and plasma LNO 2 species based on chromatographic elution and mass spectral properties. Collision-induced dissociation fragmentation patterns from synthetic LNO 2 isomers were identical to those of the two most prevalent LNO 2 positional isomers found in red cells and plasma. By using [ 13 C]LNO 2 as an internal standard, red cell free and esterified LNO 2 content was 50 ± 17 and 249 ± 104 nM, respectively. The free and esterified LNO 2 content of plasma was 79 ± 35 and 550 ± 275 nM, respectively. Nitrated fatty acids, thus, represent the single largest pool of bioactive oxides of nitrogen in the vasculature, with a net LNO 2 concentration of 477 ± 128 nM, excluding buffy coat cells. These observations affirm that basal oxidative and nitrating conditions occur in healthy humans to an extent that is sufficient to induce abundant membrane and lipoprotein–fatty acid nitration. Given that LNO 2 is capable of mediating cGMP and non-cGMP-dependent signaling reactions, fatty acid nitration products are species representing the convergence of NO and oxygenated lipid cell-signaling pathways.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Peroxynitrite can mediate lipid nitration.
“For example, lipids are also a significant biological target for peroxynitrite, and although the species can abstract a hydrogen atom to initiate lipid peroxidation, a detrimental process that can lead to cytotoxicity in many cases (65, 766), peroxynitrite can also mediate lipid nitration (54, 1027).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [54]
Nitrated fatty acids have been detected both in animal tissues subjected to inflammatory conditions and endogenously in healthy human plasma and urine.
“Nitrated fatty acids have been detected both in animal tissues subjected to inflammatory conditions and endogenously in healthy human plasma and urine (25,38).”
From Conjugated linoleic acid is a preferential substrate for fatty acid nitration (2012) · cites this paper as [25]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751 · see citing works
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/y59-099 · Cited by 23,189 · see citing works
Beckman, J. S., Beckman, T. W., Chen, J., Marshall, P. A., & Freeman, B. A. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.4.1620 · Cited by 7,029 · see citing works
Self-citationNitric oxide as a secretory product of mammalian cells
Nathan, C. (1992) · The FASEB Journal · doi:10.1096/fasebj.6.12.1381691 · Cited by 4,350 · see citing works
Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation
Cosby, K., Partovi, K. S., Crawford, J. H., Patel, R. P., Reiter, C. D., Martyr, S., Yang, B. K., Waclawiw, M. A., Zalos, G., Xu, X., et al. (2003) · Nature Medicine · doi:10.1038/nm954 · Cited by 1,807 · see citing works
Arnold, W. P., Mittal, C. K., Katsuki, S., & Murad, F. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.8.3203 · Cited by 1,459 · see citing works
Rubbo, H., Radí, R., Trujillo, M., Telleri, R., Kalyanaraman, B., Barnes, S., KIRK, M. C., & Freeman, B. Α. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47160-8 · Cited by 1,350 · see citing works
Self-citationThomas, D. D., Liu, X., Kantrow, S. P., & Lancaster, J. R. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.98.1.355 · Cited by 760 · see citing works
Myeloperoxidase, a Leukocyte-Derived Vascular NO Oxidase
Eiserich, J. P., Baldus, S. E., Brennan, M., Ma, W., Zhang, C., Tousson, A., Castro, L., Lusis, A. J., Nauseef, W. M., White, C. R., et al. (2002) · Science · doi:10.1126/science.1106830 · Cited by 685 · see citing works
Self-citationKissner, R., Nauser, T., Bugnon, P., Lye, P. G., & Koppenol, W. H. (1997) · Chemical Research in Toxicology · doi:10.1021/tx970160x · Cited by 629 · see citing works
Liu, X., Miller, M. J. S., Joshi, M. S., Thomas, D. D., & Lancaster, J. R. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.5.2175 · Cited by 602 · see citing works
Gladwin, M. T., Shelhamer, J. H., Schechter, A. N., Pease-Fye, M. E., Waclawiw, M. A., Panza, J. A., Ognibene, F. P., & Cannon, R. O. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.21.11482 · Cited by 441 · see citing works
Baldus, S. E., Eiserich, J. P., Mani, A. R., Castro, L., Figueroa, M., Chumley, P. H., Ma, W., Tousson, A., White, C. R., Bullard, D. C., et al. (2001) · Journal of Clinical Investigation · doi:10.1172/jci12617 · Cited by 332 · see citing works
Self-citationO'Donnell, V. B., Eiserich, J. P., Chumley, P. H., Jablonsky, M. J., Krishna, N. R., Kirk, M., Barnes, S., Darley-Usmar, V. M., & Freeman, B. A. (1999) · Chemical Research in Toxicology · doi:10.1021/tx980207u · Cited by 289 · see citing works
Self-citationBaldus, S. E., Eiserich, J. P., Mani, A. R., et al. (2001) · Journal of Clinical Investigation · doi:10.1172/jci200112617 · Cited by 281 · see citing works
Nitric Oxide Regulation of Tissue Free Radical Injury
Rubbo, H., Darley-Usmar, V. M., & Freeman, B. Α. (1996) · Chemical Research in Toxicology · doi:10.1021/tx960037q · Cited by 274 · see citing works
Self-citationInteractions Between Nitric Oxide and Lipid Oxidation Pathways: Implications for Vascular Disease
O’Donnell, V. B., & Freeman, B. A. (2001) · Circulation Research · doi:10.1161/01.res.88.1.12 · Cited by 257 · see citing works
Self-citationO’Donnell, V. B., Chumley, P. H., Hogg, N., Bloodsworth, A., Darley-Usmar, V. M., & Freeman, B. Α. (1997) · Biochemistry · doi:10.1021/bi971891z · Cited by 253 · see citing works
Self-citationConcomitant presence of N-nitroso and S-nitroso proteins in human plasma
Rassaf, T., Bryan, N. S., Kelm, M., & Feelisch, M. (2002) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(02)01183-8 · Cited by 192 · see citing works
Coles, B., Bloodsworth, A., Clark, S. R., Lewis, M. J., Cross, A. R., Freeman, B. A., & O’Donnell, V. B. (2002) · Circulation Research · doi:10.1161/01.res.0000032114.68919.ef · Cited by 168 · see citing works
Self-citationNO adducts in mammalian red blood cells: too much or too little?
Rassaf, T., Bryan, N. S., Maloney, R. E., Specian, V., Kelm, M., Kalyanaraman, B., Rodriguez, J., & Feelisch, M. (2003) · Nature Medicine · doi:10.1038/nm0503-481 · Cited by 151 · see citing works
Pryor, W. A., Lightsey, J. W., & Church, D. F. (1982) · Journal of the American Chemical Society · doi:10.1021/ja00388a035 · Cited by 149 · see citing works
Permeability of Nitric Oxide through Lipid Bilayer Membranes
Subczynski, W. K., Łomnicka, M., & Hyde, J. S. (1996) · Free Radical Research · doi:10.3109/10715769609088032 · Cited by 129 · see citing works
Lim, D. G., Sweeney, S., Bloodsworth, A., White, C. R., Chumley, P. H., Krishna, N. R., Schopfer, F., O'Donnell, V. B., Eiserich, J. P., & Freeman, B. A. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.232409599 · Cited by 110 · see citing works
Self-citation[37] Peroxynitrite reactions with carbon dioxide-bicarbonate
Radí, R., Denicola, A., & Freeman, B. Α. (1999) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(99)01099-x · Cited by 103 · see citing works
Self-citationCharacterization of linoleic acid nitration in human blood plasma by mass spectrometry
Lima, É. S., Di Mascio, P., Rubbo, H., & Abdalla, D. S. P. (2002) · Biochemistry · doi:10.1021/bi025504j · Cited by 103 · see citing works
Myoglobin-catalyzed hydrogen peroxide dependent arachidonic acid peroxidation
Grisham, M. B. (1985) · Journal of Free Radicals in Biology & Medicine · doi:10.1016/0748-5514(85)90122-9 · Cited by 99 · see citing works
Coles, B., Bloodsworth, A., Eiserich, J. P., Coffey, M. J., McLoughlin, R. M., Giddings, J. C., Lewis, M. J., Haslam, R. J., Freeman, B. A., & O'Donnell, V. B. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m105209200 · Cited by 98 · see citing works
Self-citationCytochrome c: a catalyst and target of nitrite-hydrogen peroxide-dependent protein nitration
Castro, L., Eiserich, J. P., Sweeney, S., Radi, R., & Freeman, B. A. (2004) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2003.08.033 · Cited by 84 · see citing works
Self-citationInteraction of Nitrite with Proteins at Gastric pH
Knowles, M. E., McWeeny, D. J., COUCHMAN, L., et al. (1974) · Nature · doi:10.1038/247288a0 · Cited by 81 · see citing works
Jourd’heuil, D., Miranda, K. M., Kim, S. M., Espey, M. G., Yoram Vodovotz, B., Laroux, S., Mai, C. T., Miles, A. M., Grisham, M. B., & Wink, D. A. (1999) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1999.1143 · Cited by 74 · see citing works
Balazy, M., Iesaki, T., Park, J. L., Jiang, H., Kaminski, P. M., & Wolin, M. S. (2001) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(24)29269-7 · Cited by 73 · see citing works
Cholesteryl nitrolinoleate, a nitrated lipid present in human blood plasma and lipoproteins
Lima, É. S., Mascio, P. D., & Abdalla, D. S. P. (2003) · Journal of Lipid Research · doi:10.1194/jlr.m200467-jlr200 · Cited by 72 · see citing works
Synthesis of conjugated nitroalkenes via nitroselenenylation of alkenes
Hayama, T., Tomoda, S., Takeuchi, Y., & Nomura, Y. (1982) · Tetrahedron Letters · doi:10.1016/s0040-4039(00)85699-3 · Cited by 67 · see citing works
Gallon, A. A., & Pryor, W. A. (1994) · Lipids · doi:10.1007/bf02536725 · Cited by 61 · see citing works
Napolitano, A., Camera, E., Picardo, M., & d'Ischia, M. (2000) · The Journal of Organic Chemistry · doi:10.1021/jo000090q · Cited by 56 · see citing works
Using Natural Isotope Variations of Nitrogen in Plants as an Early Indicator of Air Pollution Stress
Hofmann, D., Jung, K., Bender, J., Gehre, M., & Schüürmann, G. (1997) · Journal of Mass Spectrometry · doi:10.1002/(sici)1096-9888(199708)32:8 · Cited by 55 · see citing works
Söderling, A., Ryberg, H., Gabrielsson, A., Lärstad, M., Torén, K., Niari, S., & Caidahl, K. (2003) · Journal of Mass Spectrometry · doi:10.1002/jms.543 · Cited by 48 · see citing works
Gallon, A. A., & Pryor, W. A. (1993) · Lipids · doi:10.1007/bf02535776 · Cited by 45 · see citing works
Wheelan, P., Zirrolli, J. A., & Murphy, R. C. (1995) · Journal of the American Society for Mass Spectrometry · doi:10.1016/1044-0305(94)00090-m · Cited by 38 · see citing works
Tracking Components of the Transcription Apparatus in Living Cells
Baumann, C. T., & Reyes, J. C. (1999) · Methods · doi:10.1006/meth.1999.0871 · Cited by 27 · see citing works
d’Ischia, M., Rega, N., & Barone, V. (1999) · Tetrahedron · doi:10.1016/s0040-4020(99)00491-3 · Cited by 23 · see citing works
Napolitano, A., Crescenzi, O., Camera, E., Giudicianni, I., Picardo, M., & d'Ischia, M. (2002) · Tetrahedron · doi:10.1016/s0040-4020(02)00460-x · Cited by 23 · see citing works
Highly selective reduction of conjugated nitroalkenes with zinc borohydride in DME
Ranu, B. C., & Chakraborty, R. (1991) · Tetrahedron Letters · doi:10.1016/0040-4039(91)80839-x · Cited by 16 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- [22]Lim, D. G., Sweeney, S., Bloodsworth, A., White, C. R., Chumley, P. H., Krishna, N. R., Schopfer, F., O'Donnell, V. B., Eiserich, J. P. & Freeman, B. A. (2002) Proc. Natl. Acad. Sci. USA 99, 15941-15946.
- [33]Gladwin, M. T., Shelhamer, J. H., Schechter, A. N., Pease-Fye, M. E., Waclawiw, M. A., Panza, J. A., Ognibene, F. P. & Cannon, R. O., III. (2000) Proc. Natl. Acad. Sci. USA 97, 11482-11487.
Cited by
Sorted by citations
Nitric Oxide and Peroxynitrite in Health and Disease
Pacher, P., Beckman, J. S., & Liaudet, L. (2007) · Physiological Reviews · doi:10.1152/physrev.00029.2006 · Cited by 6,390 · see citing works
Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation
Tejero, J., Shiva, S., & Gladwin, M. T. (2019) · Physiological Reviews · doi:10.1152/physrev.00036.2017 · Cited by 541 · see citing works
Nitrolinoleic acid. An endogenous peroxisome proliferator-activated receptor γ ligand
Schopfer, F. J., Lin, Y., Baker, P. R. S., Cui, T., Garcia-Barrio, M., Zhang, J., Chen, K., Chen, Y. E., & Freeman, B. A. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0408384102 · Cited by 428 · see citing works
Self-citationThomas, S. R., Witting, P. K., & Drummond, G. R. (2008) · Antioxidants & Redox Signaling · doi:10.1089/ars.2008.2027 · Cited by 383 · see citing works
Fatty Acid Transduction of Nitric Oxide Signaling
Baker, P. R. S., Lin, Y., Schopfer, F. J., Woodcock, S. R., Groeger, A. L., Batthyany, C., Sweeney, S., Long, M. H., Iles, K. E., Baker, L. M. S., et al. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m504212200 · Cited by 358 · see citing works
Self-citationNitrated Fatty Acids: Endogenous Anti-inflammatory Signaling Mediators
Cui, T., Schopfer, F. J., Zhang, J., Chen, K., Ichikawa, T., Baker Paul, R. S., Batthyany, C., Chacko, B. K., Feng, X., Patel, R. P., et al. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m603357200 · Cited by 351 · see citing works
Self-citationErythrocytes are the major intravascular storage sites of nitrite in human blood
Dejam, A. (2005) · Blood · doi:10.1182/blood-2005-02-0567 · Cited by 350 · see citing works
Unraveling the Reactions of Nitric Oxide, Nitrite, and Hemoglobin in Physiology and Therapeutics
Kim-Shapiro, D. B., Schechter, A. N., & Gladwin, M. T. (2006) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.0000204350.44226.9a · Cited by 308 · see citing works
Reversible Post-translational Modification of Proteins by Nitrated Fatty Acids in Vivo
Batthyany, C., Schopfer, F. J., Baker, P. R. S., Durán, R., Baker, L. M. S., Huang, Y., Cerveñansky, C., Branchaud, B. P., & Freeman, B. A. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m602814200 · Cited by 285 · see citing works
Nitro-fatty Acid Reaction with Glutathione and Cysteine
Baker, L. M. S., Baker, P. R. S., Golin-Bisello, F., Schopfer, F. J., Fink, M., Woodcock, S. R., Branchaud, B. P., Radi, R., & Freeman, B. A. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m704085200 · Cited by 200 · see citing works
Self-citationRudolph, V., Rudolph, T. K., Schopfer, F. J., Bonacci, G., Woodcock, S. R., Cole, M. P., Baker, P. R. S., Ramani, R., & Freeman, B. A. (2010) · Cardiovascular Research · doi:10.1093/cvr/cvp275 · Cited by 188 · see citing works
Self-citationCovalent Peroxisome Proliferator-activated Receptor γ Adduction by Nitro-fatty Acids
Schopfer, F. J., Cole, M. P., Groeger, A. L., Chen, C.-S., Khoo, N. K. H., Woodcock, S. R., Golin-Bisello, F., Motanya, U. N., Li, Y., Zhang, J., et al. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.091512 · Cited by 171 · see citing works
Self-citationHickok, J. R., Sahni, S., Shen, H., Arvind, A., Antoniou, C., Fung, L. W. M., & Thomas, D. D. (2011) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2011.06.030 · Cited by 155 · see citing works
Conjugated linoleic acid is a preferential substrate for fatty acid nitration
Bonacci, G., Baker, P. R. S., Salvatore, S. R., Shores, D., Khoo, N. K. H., Koenitzer, J. R., Vitturi, D. A., Woodcock, S. R., Golin-Bisello, F., Cole, M. P., et al. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.401356 · Cited by 147 · see citing works
Self-citationVillacorta, L., Zhang, J., Garcia-Barrio, M. T., Chen, X.-L., Freeman, B. A., Chen, Y. E., & Cui, T. (2007) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00261.2007 · Cited by 144 · see citing works
Self-citationNadtochiy, S. M., Baker, P. R. S., Freeman, B. A., & Brookes, P. S. (2009) · Cardiovascular Research · doi:10.1093/cvr/cvn323 · Cited by 131 · see citing works
Self-citationNitrite Reduction and Cardiovascular Protection
Omar, S. A., & Webb, A. J. (2014) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2014.01.012 · Cited by 121 · see citing works
Peroxynitrite-mediated lipid oxidation and nitration: mechanisms and consequences
Rubbo, H., Trostchansky, A., & O’Donnell, V. B. (2009) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2008.11.007 · Cited by 105 · see citing works
Schopfer, F. J., Batthyany, C., Baker, P. R. S., Bonacci, G., Cole, M. P., Rudolph, V., Groeger, A. L., Rudolph, T. K., Nadtochiy, S., Brookes, P. S., et al. (2009) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2008.12.025 · Cited by 104 · see citing works
Self-citationCharles, R. L., Rudyk, O., Prysyazhna, O., Kamynina, A., Yang, J., Morisseau, C., Hammock, B. D., Freeman, B. A., & Eaton, P. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1402965111 · Cited by 100 · see citing works
Nitrated oleic acid up-regulates PPARγ and attenuates experimental inflammatory bowel disease
Borniquel, S., Jansson, E. Å., Cole, M. P., Freeman, B. A., & Lundberg, J. O. (2010) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2009.11.014 · Cited by 92 · see citing works
Self-citationCharacterization and quantification of endogenous fatty acid nitroalkene metabolites in human urine
Salvatore, S. R., Vitturi, D. A., Baker, P. R. S., Bonacci, G., Koenitzer, J. R., Woodcock, S. R., Freeman, B. A., & Schopfer, F. J. (2013) · Journal of Lipid Research · doi:10.1194/jlr.m037804 · Cited by 82 · see citing works
Self-citationNitro-oleic Acid, a Novel and Irreversible Inhibitor of Xanthine Oxidoreductase
Kelley, E. E., Batthyany, C. I., Hundley, N. J., Woodcock, S. R., Bonacci, G., Del Rio, J. M., Schopfer, F. J., Lancaster, J. R., Freeman, B. A., & Tarpey, M. M. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m802402200 · Cited by 81 · see citing works
Self-citationMacrophage activation induces formation of the anti-inflammatory lipid cholesteryl-nitrolinoleate
Ferreira, A. M., Ferrari, M. I., Trostchansky, A., Batthyany, C., Souza, J. M., Alvarez, M. N., López, G. V., Baker, P. R. S., Schopfer, F. J., O'Donnell, V., et al. (2009) · Biochemical Journal · doi:10.1042/bj20080701 · Cited by 78 · see citing works
Self-citationNitroalkenes Confer Acute Cardioprotection via Adenine Nucleotide Translocase 1
Nadtochiy, S. M., Zhu, Q., Urciuoli, W., Rafikov, R., Black, S. M., & Brookes, P. S. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.298406 · Cited by 55 · see citing works
The nitrated fatty acid 10-nitro-oleate attenuates allergic airway disease
Reddy, A. T., Lakshmi, S. P., Dornadula, S., Pinni, S., Rampa, D. R., & Reddy, R. C. (2013) · The Journal of Immunology · doi:10.4049/jimmunol.1300730 · Cited by 42 · see citing works
Nitro-fatty acids as novel electrophilic ligands for peroxisome proliferator-activated receptors
Ferreira, A. M., Minarrieta, L., Lamas Bervejillo, M., & Rubbo, H. (2012) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2012.08.572 · Cited by 16 · see citing works
Bates, D. J. P., Smitherman, P. K., Townsend, A. J., King, S. B., & Morrow, C. S. (2011) · Biochemistry · doi:10.1021/bi2005784 · Cited by 15 · see citing works
- Authors
- Baker, P. R. S., Schopfer, F. J., Sweeney, S., & Freeman, B. A.
- Affiliations
- University of Alabama at Birmingham (US)
- University of California, Los Angeles (US)
- Departments of Anesthesiology, Biochemistry, and Molecular Genetics and Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, AL 35294
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2004-07-23
- Volume
- 101
- Issue
- 32
- Pages
- 11577-11582
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC511023
- OpenAlex
- W2137704083
- Cited by
- 201
- References
- 44
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 3 · 2020: 9 · 2019: 7 · 2018: 7 · 2017: 7 · 2016: 7 · 2015: 7 · 2014: 4 · 2013: 12 · 2012: 13
- FWCI
- 8.62
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wake Forest University
- National Institutes of Health
- National Heart, Lung, and Blood Institute (R01HL64937, R01HL064937, R01HL058115, R01-HL-58115, T32-HL-07457, T32HL007457)
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- nitrated fatty acids, plasma lipids, nitric oxide metabolites, nitric oxide, esterified fatty acids, regioisomers, 13C-labeled internal standards, lipid oxidation, signal transduction, cGMP signaling, nitrosation
- MeSH terms
- Chromatography, High Pressure Liquid, Erythrocyte Membrane, Fatty Acids, Unsaturated, Humans, Isomerism, Nitro Compounds, Mass Spectrometry, Linoleic Acid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
