Nitrolinoleate Inhibits Superoxide Generation, Degranulation, and Integrin Expression by Human Neutrophils: Novel Antiinflammatory Properties of Nitric Oxide–Derived Reactive Species in Vascular Cells
Abstract
Nitration of unsaturated fatty acids such as linoleate by NO-derived reactive species forms novel derivatives (including nitrolinoleate [LNO2]) that can stimulate smooth muscle relaxation and block platelet activation by either NO/cGMP or cAMP-dependent mechanisms. Here, LNO2 was observed to inhibit human neutrophil function. LNO2, but not linoleic acid or the nitrated amino acid 3-nitrotyrosine, dose-dependently (0.2 to 1 micromol/L) inhibited superoxide (O2*-) generation, Ca2+ influx, elastase release, and CD11b expression in response to either phorbol 12-myristate 13-acetate or N-formyl-Met-Leu-Phe. LNO2 did not elevate cGMP, and inhibition of guanylate cyclase by 1H-[1,2,4]oxadiazole[4,3-a]quinoxalin-1-one did not restore neutrophil responses, ruling out a role for NO. In contrast, LNO2 caused elevations in intracellular cAMP in the presence and absence of phosphodiesterase inhibition, suggesting activation of adenylate cyclase. Compared with phorbol 12-myristate 13-acetate-activated neutrophils, N-formyl-Met-Leu-Phe-activated neutrophils were more susceptible to the inhibitory effects of LNO2, indicating that LNO2 may inhibit signaling both upstream and downstream of protein kinase C. These data suggest novel signaling actions for LNO2 in mediating its potent inhibitory actions. Thus, nitration of lipids by NO-derived reactive species yields products with antiinflammatory properties, revealing a novel mechanism by which NO-derived nitrated biomolecules can influence the progression of vascular disease.
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Nitroalkene derivatives of fatty acids (NO2-FA) limit inflammatory responses by non-cGMP-dependent inhibition of platelet and neutrophil function and by inhibiting the catalytic activity and gene expression of cyclooxygenase-2 and xanthine oxidoreductase.
“Finally, NO2-FA limit inflammatory responses by non-cGMP-dependent inhibition of platelet and neutrophil function [20], [21] and by inhibiting the catalytic activity and gene expression of the pro-inflammatory proteins cyclooxygenase-2 and xanthine oxidoreductase [22], [23].”
From Olives and olive oil are sources of electrophilic fatty acid nitroalkenes (2014) · cites this paper as [20]
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- Authors
- Coles, B., Bloodsworth, A., Clark, S. R., Lewis, M. J., Cross, A. R., Freeman, B. A., & O’Donnell, V. B.
- Affiliations
- Scripps Research Institute (US)
- From the Department of Medical Biochemistry (B.C., S.R.C., V.B.O.) and the Department of Pharmacology, Therapeutics, and Toxicology (M.J.L.), University of Wales College of Medicine, Cardiff, UK; the Department of Molecular and Experimental Medicine (A.R.C.), The Scripps Research Institute, La Jolla, Calif; and the Departments of Anesthesiology, Biochemistry, and Molecular Genetics and the Center for Free Radical Biology (A.B., B.A.F.), University of Alabama at Birmingham.
- Venue
- Circulation Research
- Published
- 2002-09-06
- Volume
- 91
- Issue
- 5
- Pages
- 375-381
- ISSN
- 0009-7330 (ISSN-L)
- 1524-4571
- OpenAlex
- W2170339244
- Cited by
- 168
- References
- 42
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 6 · 2020: 2 · 2019: 6 · 2018: 6 · 2017: 4 · 2016: 4 · 2015: 7 · 2014: 9 · 2013: 12 · 2012: 9
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Circulation Research (publishedVersion)
- PubMed (publishedVersion)
- ORCA Online Research @Cardiff (Cardiff University) (acceptedVersion)
- Funders
- Wellcome Trust
- British Heart Foundation
- National Institutes of Health (5p60hl058418-05)
- U.S. Public Health Service (R01-A124838)
- National Heart, Lung, and Blood Institute (R01HL64937, R01HL064937, P6-HL-58418, R01HL058115, R01-HL-58115)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- neutrophils, superoxide production, neutrophil degranulation, elastase release, intracellular cAMP, adenylate cyclase, protein kinase C signaling, nitric oxide, anti-inflammatory signaling, vascular disease
- MeSH terms
- Cyclic AMP, Anti-Inflammatory Agents, Calcium, Dose-Response Relationship, Drug, Cyclic GMP, Humans, N-Formylmethionine Leucyl-Phenylalanine, Neutrophils, Nitro Compounds, Phosphodiesterase Inhibitors, Superoxides, Tetradecanoylphorbol Acetate, 1-Methyl-3-isobutylxanthine, Cell Degranulation, Integrins, NADPH Oxidases, Linoleic Acid
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