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Mast cells are dispensable for normal and activin‐promoted wound healing and skin carcinogenesis

Antsiferova, Maria, Martin, Caroline, Huber, Marcel, Feyerabend, Thorsten B, Förster, Anja, Hartmann, Karin, Rodewald, Hans-Reimer, Hohl, Daniel, Werner, Sabine·2013·The Journal of Immunology
DOI 10.4049/jimmunol.1301350
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Abstract

The growth and differentiation factor activin A is a key regulator of tissue repair, inflammation, fibrosis, and tumorigenesis. However, the cellular targets, which mediate the different activin functions, are still largely unknown. In this study, we show that activin increases the number of mature mast cells in mouse skin in vivo. To determine the relevance of this finding for wound healing and skin carcinogenesis, we mated activin transgenic mice with CreMaster mice, which are characterized by Cre recombinase-mediated mast cell eradication. Using single- and double-mutant mice, we show that loss of mast cells neither affected the stimulatory effect of overexpressed activin on granulation tissue formation and reepithelialization of skin wounds nor its protumorigenic activity in a model of chemically induced skin carcinogenesis. Furthermore, mast cell deficiency did not alter wounding-induced inflammation and new tissue formation or chemically induced angiogenesis and tumorigenesis in mice with normal activin levels. These findings reveal that mast cells are not major targets of activin during wound healing and skin cancer development and also argue against nonredundant functions of mast cells in wound healing and skin carcinogenesis in general.

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Research works printed but not linked: 39

  1. Rodewald, 2012, Widespread immunological functions of mast cells: fact or fiction?, Immunity, 37, 13, 10.1016/j.immuni.2012.07.007 · DOI · Paper page
  2. Noli, 2001, The mast cell in wound healing, Vet. Dermatol., 12, 303, 10.1046/j.0959-4493.2001.00272.x · DOI
  3. Nocka, 1990, Molecular bases of dominant negative and loss of function mutations at the murine c-kit/white spotting locus: W37, Wv, W41 and W, EMBO J., 9, 1805, 10.1002/j.1460-2075.1990.tb08305.x · DOI
  4. Egozi, 2003, Mast cells modulate the inflammatory but not the proliferative response in healing wounds, Wound Repair Regen., 11, 46, 10.1046/j.1524-475X.2003.11108.x · DOI
  5. Iba, 2004, Possible involvement of mast cells in collagen remodeling in the late phase of cutaneous wound healing in mice, Int. Immunopharmacol., 4, 1873, 10.1016/j.intimp.2004.08.009 · DOI
  6. Nauta, 2013, Evidence that mast cells are not required for healing of splinted cutaneous excisional wounds in mice, PLoS One, 8, e59167, 10.1371/journal.pone.0059167 · DOI
  7. Weller, 2006, Mast cells are required for normal healing of skin wounds in mice, FASEB J., 20, 2366, 10.1096/fj.06-5837fje · DOI
  8. Feyerabend, 2011, Cre-mediated cell ablation contests mast cell contribution in models of antibody- and T cell-mediated autoimmunity, Immunity, 35, 832, 10.1016/j.immuni.2011.09.015 · DOI
  9. Hübner, 1996, Strong induction of activin expression after injury suggests an important role of activin in wound repair, Dev. Biol., 173, 490, 10.1006/dbio.1996.0042 · DOI
  10. Antsiferova, 2011, Activin enhances skin tumourigenesis and malignant progression by inducing a pro-tumourigenic immune cell response, Nat. Commun., 2, 576, 10.1038/ncomms1585 · DOI
  11. Funaba, 2003, Role of activin A in murine mast cells: modulation of cell growth, differentiation, and migration, J. Leukoc. Biol., 73, 793, 10.1189/jlb.0103012 · DOI
  12. Munz, 1999, Overexpression of activin A in the skin of transgenic mice reveals new activities of activin in epidermal morphogenesis, dermal fibrosis and wound repair, EMBO J., 18, 5205, 10.1093/emboj/18.19.5205 · DOI
  13. Bamberger, 2005, Activin controls skin morphogenesis and wound repair predominantly via stromal cells and in a concentration-dependent manner via keratinocytes, Am. J. Pathol., 167, 733, 10.1016/S0002-9440(10)62047-0 · DOI
  14. Munz, 1999, A novel role of activin in inflammation and repair, J. Endocrinol., 161, 187, 10.1677/joe.0.1610187 · DOI
  15. Junankar, 2006, Analysis of immune cell infiltrates during squamous carcinoma development, J. Investig. Dermatol. Symp. Proc., 11, 36, 10.1038/sj.jidsymp.5650024 · DOI
  16. Werner, 1994, The function of KGF in morphogenesis of epithelium and reepithelialization of wounds, Science, 266, 819, 10.1126/science.7973639 · DOI
  17. Schindelin, 2012, Fiji: an open-source platform for biological-image analysis, Nat. Methods, 9, 676, 10.1038/nmeth.2019 · DOI · Paper page
  18. Bradley, 1982, Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker, J. Invest. Dermatol., 78, 206, 10.1111/1523-1747.ep12506462 · DOI
  19. Conti, 1998, Mast cell recruitment after subcutaneous injection of RANTES in the sole of the rat paw, Br. J. Haematol., 103, 798, 10.1046/j.1365-2141.1998.01060.x · DOI
  20. Jones, 2012, Activin A and TGF-beta promote T(H)9 cell-mediated pulmonary allergic pathology, J. Allergy Clin. Immunol., 129, 1000, 10.1016/j.jaci.2011.12.965 · DOI
  21. Ponvert, 1985, Tissue histamine levels in male and female mast cell deficient mice (W/Wv) and in their littermates (Wv/+, W/+ and +/+), Agents Actions, 17, 1, 10.1007/BF01966671 · DOI
  22. Koizumi, 1987, Single-locus control of the mast cell population in mouse skin, Immunogenetics, 26, 36, 10.1007/BF00345452 · DOI
  23. Miller, 2002, Tissue-specific expression of mast cell granule serine proteinases and their role in inflammation in the lung and gut, Immunology, 105, 375, 10.1046/j.1365-2567.2002.01375.x · DOI
  24. Funaba, 2005, Up-regulation of mouse mast cell protease-6 gene by transforming growth factor-beta and activin in mast cell progenitors, Cell. Signal., 17, 121, 10.1016/j.cellsig.2004.06.005 · DOI
  25. Wang, 2013, The mouse excisional wound splinting model, including applications for stem cell transplantation, Nat. Protoc., 8, 302, 10.1038/nprot.2013.002 · DOI
  26. Kümin, 2007, Peroxiredoxin 6 is required for blood vessel integrity in wounded skin, J. Cell Biol., 179, 747, 10.1083/jcb.200706090 · DOI
  27. Schäfer, 2008, Cancer as an overhealing wound: an old hypothesis revisited, Nat. Rev. Mol. Cell Biol., 9, 628, 10.1038/nrm2455 · DOI
  28. Antsiferova, 2012, The bright and the dark sides of activin in wound healing and cancer, J. Cell Sci., 125, 3929
  29. de Kretser, 2012, The roles of activin A and its binding protein, follistatin, in inflammation and tissue repair, Mol. Cell. Endocrinol., 359, 101, 10.1016/j.mce.2011.10.009 · DOI
  30. Mukhopadhyay, 2007, The role of the activin system in keloid pathogenesis, Am. J. Physiol. Cell Physiol., 292, C1331, 10.1152/ajpcell.00373.2006 · DOI
  31. Werner, 2006, Roles of activin in tissue repair, fibrosis, and inflammatory disease, Cytokine Growth Factor Rev., 17, 157, 10.1016/j.cytogfr.2006.01.001 · DOI · Paper page
  32. Otranto, 2012, The role of the myofibroblast in tumor stroma remodeling, Cell Adhes. Migr., 6, 203, 10.4161/cam.20377 · DOI
  33. Bernstein, 1990, The mouse W/c-kit locus, Ciba Found. Symp., 148, 158
  34. Zhou, 2007, Mast cell deficiency in Kit(W-sh) mice does not impair antibody-mediated arthritis, J. Exp. Med., 204, 2797, 10.1084/jem.20071391 · DOI
  35. Wulff, 2012, Mast cells contribute to scar formation during fetal wound healing, J. Invest. Dermatol., 132, 458, 10.1038/jid.2011.324 · DOI
  36. de Souza, 2012, Expression of mast cell proteases correlates with mast cell maturation and angiogenesis during tumor progression, PLoS One, 7, e40790, 10.1371/journal.pone.0040790 · DOI
  37. Muto, 2007, Decreased c-kit function inhibits enhanced skin carcinogenesis in c-Ha-ras protooncogene transgenic mice, Cancer Sci., 98, 1549, 10.1111/j.1349-7006.2007.00577.x · DOI
  38. Gordon, 1990, Phorbol 12-myristate 13-acetate-induced development of functionally active mast cells in W/Wv but not Sl/Sld genetically mast cell-deficient mice, Blood, 75, 1637, 10.1182/blood.V75.8.1637.1637 · DOI
  39. Waskow, 2007, Kit is essential for PMA-inflammation-induced mast-cell accumulation in the skin, Blood, 109, 5363, 10.1182/blood-2006-08-039131 · DOI

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Metadata

Authors
Antsiferova, M., Martin, C., Huber, M., Feyerabend, T. B., Förster, A., Hartmann, K., Rodewald, H.-R., Hohl, D., & Werner, S.
Affiliations
  • Department of Biology, Institute of Molecular Health Sciences, Swiss Federal Institute of Technology Zurich , 8093 Zürich,
  • Department of Dermatology, University of Lausanne , 1011 Lausanne,
  • German Cancer Research Center , 69120 Heidelberg,
  • Department of Dermatology, University of Cologne , 50937 Cologne,
Venue
The Journal of Immunology
Publisher
Oxford University Press (OUP)
Published
2013-12
Volume
191
Issue
12
Pages
6147-6155
ISSN
  • 0022-1767
  • 1550-6606
DOI
10.4049/jimmunol.1301350
Cited by
75
References
1
Language
en
Licence
academic.oup.com/pages/standard-publication-reuse-rights (vor)

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CoChat. (2026). Citation check: Mast cells are dispensable for normal and activin‐promoted wound healing and skin carcinogenesis. https://app.cochat.ai/papers/10.4049/jimmunol.1301350
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Antsiferova, M., Martin, C., Huber, M., Feyerabend, T. B., Förster, A., Hartmann, K., Rodewald, H.-R., Hohl, D., & Werner, S. (2013). Mast cells are dispensable for normal and activin‐promoted wound healing and skin carcinogenesis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.1301350