Biogenesis of phagolysosomes proceeds through a sequential series of interactions with the endocytic apparatus
Open accessAbstract
We have examined the modifications occurring during the transformation of phagosomes into phagolysosomes in J-774 macrophages. The use of low density latex beads as markers of phagosomes (latex bead compartments, LBC) allowed the isolation of these organelles by flotation on a simple sucrose gradient. Two-dimensional gel electrophoresis, immunocytochemistry, and biochemical assays have been used to characterize the composition of LBC at different time points after their formation, as well as their interactions with the organelles of the endocytic pathway. Our results show that LBC acquire and lose various markers during their transformation into phagolysosomes. Among these are members of the rab family of small GTPases as well as proteins of the lamp family. The transfer of the LBC of lamp 2, a membrane protein associated with late endocytic structures, was shown to be microtubule dependent. Video-microscopy showed that newly formed phagosomes were involved in rapid multiple contacts with late components of the endocytic pathway. Collectively, these observations suggest that phagolysosome formation is a highly dynamic process that involves the gradual and regulated acquisition of markers from endocytic organelles.
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The movement of phagosomes along microtubules likely facilitates the interaction of phagosomes with endosomes and lysosomes.
“Earlier studies on the phagocytosis of latex beads by macrophages concluded that the movement of the phagosomes along microtubules likely facilitates the interaction of phagosomes with endosomes and lysosomes (Rabinowitz et al., 1992; Desjardins et al., 1994).”
From Microtubule motors transport phagosomes in the RPE, and lack of KLC1 leads to AMD-like pathogenesis (2015) · cites this paper as Desjardins et al., 1994
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- Authors
- Desjardins, M., Huber, L., Parton, R., & Griffiths, G.
- Affiliations
- European Molecular Biology Laboratory (DE)
- European Molecular Biology Laboratory, Cell Biology Program, Heidelberg, Germany.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1994-03-01
- Volume
- 124
- Issue
- 5
- Pages
- 677-688
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2119957
- OpenAlex
- W2049288737
- Cited by
- 673
- References
- 44
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 10 · 2023: 12 · 2022: 14 · 2021: 12 · 2020: 12 · 2019: 16 · 2018: 10 · 2017: 16 · 2016: 12 · 2015: 14 · 2014: 24 · 2013: 26 · 2012: 18
- FWCI
- 8.02
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
- Keywords
- phagolysosome biogenesis, phagosome maturation, endocytic pathway, Rab GTPases, LAMP2, microtubule transport, late endosomes
- MeSH terms
- Animals, Cell Fractionation, Cell Line, Centrifugation, Density Gradient, Endocytosis, Guanosine Triphosphate, Immunohistochemistry, Kinetics, Lysosomes, Macrophages, Methionine, Microscopy, Electron, Microtubules, Phagocytosis, Proteins, Protein Biosynthesis, Video Recording, Immunoblotting, Electrophoresis, Gel, Two-Dimensional, Organelles, Mice
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