The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Human Toll-Like Receptor 2 Mediates Monocyte Activation by Listeria monocytogenes , But Not by Group B Streptococci or Lipopolysaccharide
Open accessAbstract
Human Toll like receptor (TLR) 2 has been implicated as a signaling receptor for LPS from Gram-negative bacteria and cell wall components from Gram-positive organisms. In this study, we investigated whether TLR2 can signal cell activation by the heat-killed group B streptococci type III (GBS) and Listeria monocytogenes (HKLM). HKLM, but not GBS, showed a time- and dose-dependent activation of Chinese hamster ovary cells transfected with human TLR2, as measured by translocation of NF-κB and induction of IL-6 production. A mAb recognizing a TLR2-associated epitope (TL2.1) was generated that inhibited IL-6 production from Chinese hamster ovary-TLR2 cells stimulated with HKLM or LPS. The TL2.1 mAb reduced HKLM-induced TNF production from human monocytes by 60%, whereas a CD14 mAb (3C10) reduced the TNF production by 30%. However, coadministrating TL2.1 and 3C10 inhibited the TNF response by 80%. In contrast to this, anti-CD14 blocked LPS-induced TNF production from monocytes, whereas anti-TLR2 showed no inhibition. Neither TL2.1 nor 3C10 affected GBS-induced TNF production. These results show that TLR2 can function as a signaling receptor for HKLM, possibly together with CD14, but that TLR2 is unlikely to be involved in cell activation by GBS. Furthermore, although LPS can activate transfected cell lines through TLR2, this receptor does not seem to be the main transducer of LPS activation of human monocytes. Thus, our data demonstrate the ability of TLR2 to distinguish between different pathogens.
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Cites
Peptidoglycan- and Lipoteichoic Acid-induced Cell Activation Is Mediated by Toll-like Receptor 2
Schwandner, R., Dziarski, R., Wesche, H., Rothe, M., & Kirschning, C. J. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.25.17406 · Cited by 1,704
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A Novel Host-Parasite Lipid Cross-talk
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Self-citationZhang, R., Miller, R. G., Gascon, R., Champion, S., Katz, J., Lancero, M., Narvaez, A., Honrada, R., Ruvalcaba, D., & McGrath, M. S. (2009) · Journal of Neuroimmunology · doi:10.1016/j.jneuroim.2008.09.017 · Cited by 319
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Toll-Like Receptor 2 Is Required for Optimal Control of Listeria monocytogenes Infection
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Self-citationWang, Z., Mascarenhas, N., Eckmann, L., Miyamoto, Y., Sun, X., Kawakami, T., & Di Nardo, A. (2017) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2016.09.019 · Cited by 117
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Self-citationFunctionality of HDL: Antioxidation and Detoxifying Effects
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Metadata
- Authors
- Flo, T. H., Halaas, Ø., Lien, E., Ryan, L., Teti, G., Golenbock, D. T., Sundan, A., & Espevik, T.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-02
- Volume
- 164
- Issue
- 4
- Pages
- 2064-2069
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1534699340
- Cited by
- 282
- References
- 1
- FWCI
- 12.94
- Type
- journal article
- Language
- en
- Open access
- bronze
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
