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Rac2 Regulates the Migration of T Lymphoid Progenitors to the Thymus during Zebrafish Embryogenesis

Lu, Xinyan, Zhang, Yuanlin, Liu, Feng, Wang, Lu·2020·The Journal of Immunology
DOI 10.4049/jimmunol.1901494
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Abstract

The caudal hematopoietic tissue in zebrafish, the equivalent to the fetal liver in mammals, is an intermediate hematopoietic niche for the maintenance and differentiation of hematopoietic stem and progenitor cells before homing to the thymus and kidney marrow. As one of the ultimate hematopoietic organs, the thymus sustains T lymphopoiesis, which is essential for adaptive immune system. However, the mechanism of prethymic T lymphoid progenitors migrating to the thymus remains elusive. In this study, we identify an Rho GTPase Rac2 as a modulator of T lymphoid progenitor homing to the thymus in zebrafish. rac2-Deficient embryos show the inability of T lymphoid progenitors homing to the thymus because of defective cell–autonomous motility. Mechanistically, we demonstrate that Rac2 regulates homing of T lymphoid progenitor through Pak1-mediated AKT pathway. Taken together, our work reveals an important function of Rac2 in directing T lymphoid progenitor migration to the thymus during zebrafish embryogenesis.

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Research works printed but not linked: 55

  1. Murayama, 2006, Tracing hematopoietic precursor migration to successive hematopoietic organs during zebrafish development, Immunity, 25, 963, 10.1016/j.immuni.2006.10.015 · DOI
  2. Tamplin, 2015, Hematopoietic stem cell arrival triggers dynamic remodeling of the perivascular niche, Cell, 160, 241, 10.1016/j.cell.2014.12.032 · DOI
  3. Wang, 2015, Irf4 regulates the choice between T lymphoid-primed progenitor and myeloid lineage fates during embryogenesis, Dev. Cell, 34, 621, 10.1016/j.devcel.2015.07.011 · DOI
  4. Iwanami, 2011, Genetic evidence for an evolutionarily conserved role of IL-7 signaling in T cell development of zebrafish, J. Immunol., 186, 7060, 10.4049/jimmunol.1003907 · DOI
  5. Ma, 2012, Foxn1 maintains thymic epithelial cells to support T-cell development via mcm2 in zebrafish, Proc. Natl. Acad. Sci. USA, 109, 21040, 10.1073/pnas.1217021110 · DOI
  6. Sambandam, 2005, Notch signaling controls the generation and differentiation of early T lineage progenitors. [Published erratum appears in 2005 Nat. Immunol. 6: 852.], Nat. Immunol., 6, 663, 10.1038/ni1216 · DOI
  7. Bajoghli, 2009, Evolution of genetic networks underlying the emergence of thymopoiesis in vertebrates, Cell, 138, 186, 10.1016/j.cell.2009.04.017 · DOI · Paper page
  8. Benz, 2004, Homing of immature thymocytes to the subcapsular microenvironment within the thymus is not an absolute requirement for T cell development, Eur. J. Immunol., 34, 3652, 10.1002/eji.200425248 · DOI
  9. Jenkinson, 2007, Chemokine receptor expression defines heterogeneity in the earliest thymic migrants, Eur. J. Immunol., 37, 2090, 10.1002/eji.200737212 · DOI
  10. Liu, 2006, Coordination between CCR7- and CCR9-mediated chemokine signals in prevascular fetal thymus colonization, Blood, 108, 2531, 10.1182/blood-2006-05-024190 · DOI
  11. Wilkinson, 1999, Factors regulating stem cell recruitment to the fetal thymus, J. Immunol., 162, 3873, 10.4049/jimmunol.162.7.3873 · DOI
  12. Wurbel, 2001, Mice lacking the CCR9 CC-chemokine receptor show a mild impairment of early T- and B-cell development and a reduction in T-cell receptor gammadelta(+) gut intraepithelial lymphocytes, Blood, 98, 2626, 10.1182/blood.V98.9.2626 · DOI
  13. Esain, 2015, Cannabinoid receptor-2 regulates embryonic hematopoietic stem cell development via prostaglandin E2 and P-selectin activity, Stem Cells, 33, 2596, 10.1002/stem.2044 · DOI
  14. Schorpp, 2006, Conserved functions of Ikaros in vertebrate lymphocyte development: genetic evidence for distinct larval and adult phases of T cell development and two lineages of B cells in zebrafish, J. Immunol., 177, 2463, 10.4049/jimmunol.177.4.2463 · DOI
  15. Etienne-Manneville, 2002, Rho GTPases in cell biology, Nature, 420, 629, 10.1038/nature01148 · DOI · Paper page
  16. Williams, 2008, Rho GTPases and regulation of hematopoietic stem cell localization, Methods Enzymol., 439, 365, 10.1016/S0076-6879(07)00427-2 · DOI · Paper page
  17. Jarboui, 2011, Proteomic profiling of the human T-cell nucleolus, Mol. Immunol., 49, 441, 10.1016/j.molimm.2011.09.005 · DOI
  18. Rosowski, 2016, Rac2 functions in both neutrophils and macrophages to mediate motility and host defense in larval zebrafish, 4780
  19. Deng, 2011, Dual roles for Rac2 in neutrophil motility and active retention in zebrafish hematopoietic tissue, Dev. Cell, 21, 735, 10.1016/j.devcel.2011.07.013 · DOI
  20. Guo, 2008, Rac GTPase isoforms Rac1 and Rac2 play a redundant and crucial role in T-cell development, Blood, 112, 1767, 10.1182/blood-2008-01-132068 · DOI · Paper page
  21. Faroudi, 2010, Critical roles for Rac GTPases in T-cell migration to and within lymph nodes, Blood, 116, 5536, 10.1182/blood-2010-08-299438 · DOI
  22. Kimmel, 1995, Stages of embryonic development of the zebrafish, Dev. Dyn., 203, 253, 10.1002/aja.1002030302 · DOI · Paper page
  23. Zhang, 2015, G protein-coupled receptor 183 facilitates endothelial-to-hematopoietic transition via Notch1 inhibition, Cell Res., 25, 1093, 10.1038/cr.2015.109 · DOI
  24. North, 2007, Prostaglandin E2 regulates vertebrate haematopoietic stem cell homeostasis, Nature, 447, 1007, 10.1038/nature05883 · DOI · Paper page
  25. Renshaw, 2006, A transgenic zebrafish model of neutrophilic inflammation, Blood, 108, 3976, 10.1182/blood-2006-05-024075 · DOI
  26. Lin, 2005, Analysis of thrombocyte development in CD41-GFP transgenic zebrafish, Blood, 106, 3803, 10.1182/blood-2005-01-0179 · DOI
  27. Proulx, 2010, Cranial vasculature in zebrafish forms by angioblast cluster-derived angiogenesis, Dev. Biol., 348, 34, 10.1016/j.ydbio.2010.08.036 · DOI
  28. Langenau, 2004, In vivo tracking of T cell development, ablation, and engraftment in transgenic zebrafish, Proc. Natl. Acad. Sci. USA, 101, 7369, 10.1073/pnas.0402248101 · DOI
  29. Langenau, 2003, Myc-induced T cell leukemia in transgenic zebrafish, Science, 299, 887, 10.1126/science.1080280 · DOI
  30. Hall, 2007, The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish, BMC Dev. Biol., 7, 42, 10.1186/1471-213X-7-42 · DOI
  31. Zou, 2011, Two functional microRNA-126s repress a novel target gene p21-activated kinase 1 to regulate vascular integrity in zebrafish. [Published erratum appears in 2011 Circ. Res. 108: e10.], Circ. Res., 108, 201, 10.1161/CIRCRESAHA.110.225045 · DOI
  32. Kwan, 2007, The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs, Dev. Dyn., 236, 3088, 10.1002/dvdy.21343 · DOI
  33. Wang, 2011, A blood flow-dependent klf2a-NO signaling cascade is required for stabilization of hematopoietic stem cell programming in zebrafish embryos, Blood, 118, 4102, 10.1182/blood-2011-05-353235 · DOI
  34. Brend, 2009, Zebrafish whole mount high-resolution double fluorescent in situ hybridization, J. Vis. Exp., 10.3791/1229-v · DOI
  35. Zhang, 2017, m6A modulates haematopoietic stem and progenitor cell specification, Nature, 549, 273, 10.1038/nature23883 · DOI
  36. Wei, 2016, RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish, Development, 143, 2103
  37. Choorapoikayil, 2014, Pivotal role of Pten in the balance between proliferation and differentiation of hematopoietic stem cells in zebrafish, Blood, 123, 184, 10.1182/blood-2013-05-501544 · DOI
  38. Wan, 2014, Vav1 increases Bcl-2 expression by selective activation of Rac2-Akt in leukemia T cells, Cell. Signal., 26, 2202, 10.1016/j.cellsig.2014.05.015 · DOI
  39. Cancelas, 2006, The role of chemokine activation of Rac GTPases in hematopoietic stem cell marrow homing, retention, and peripheral mobilization, Exp. Hematol., 34, 976, 10.1016/j.exphem.2006.03.016 · DOI
  40. Zou, 2012, Store-operated Ca2+ entry (SOCE) plays a role in the polarization of neutrophil-like HL-60 cells by regulating the activation of Akt, Src, and Rho family GTPases, Cell. Physiol. Biochem., 30, 221, 10.1159/000339059 · DOI
  41. Higuchi, 2008, Scaffolding function of PAK in the PDK1-Akt pathway, Nat. Cell Biol., 10, 1356, 10.1038/ncb1795 · DOI
  42. Zhang, 2009, Human neutrophils coordinate chemotaxis by differential activation of Rac1 and Rac2, J. Immunol., 183, 2718, 10.4049/jimmunol.0900849 · DOI · Paper page
  43. Bleul, 2000, Chemokines define distinct microenvironments in the developing thymus, Eur. J. Immunol., 30, 3371, 10.1002/1521-4141(2000012)30:12<3371::AID-IMMU3371>3.0.CO;2-L · DOI
  44. Zhang, 2011, cMyb regulates hematopoietic stem/progenitor cell mobilization during zebrafish hematopoiesis, Blood, 118, 4093, 10.1182/blood-2011-03-342501 · DOI
  45. Seitz, 2005, The role of sphingosine 1-phosphate receptors in the trafficking of hematopoietic progenitor cells, Ann. N. Y. Acad. Sci., 1044, 84, 10.1196/annals.1349.011 · DOI
  46. Ratajczak, 2010, Novel insight into stem cell mobilization-plasma sphingosine-1-phosphate is a major chemoattractant that directs the egress of hematopoietic stem progenitor cells from the bone marrow and its level in peripheral blood increases during mobilization due to activation of complement cascade/membrane attack complex, Leukemia, 24, 976, 10.1038/leu.2010.53 · DOI
  47. Gossens, 2009, Thymic progenitor homing and lymphocyte homeostasis are linked via S1P-controlled expression of thymic P-selectin/CCL25, J. Exp. Med., 206, 761, 10.1084/jem.20082502 · DOI
  48. Croker, 2002, Rac2-deficient mice display perturbed T-cell distribution and chemotaxis, but only minor abnormalities in T(H)1 responses, Immunol. Cell Biol., 80, 231, 10.1046/j.1440-1711.2002.01077.x · DOI
  49. Dorrance, 2013, The Rac GTPase effector p21-activated kinase is essential for hematopoietic stem/progenitor cell migration and engraftment, Blood, 121, 2474, 10.1182/blood-2012-10-460709 · DOI · Paper page
  50. Accetta, 2011, Human phagocyte defect caused by a Rac2 mutation detected by means of neonatal screening for T-cell lymphopenia, J. Allergy Clin. Immunol., 127, 535, 10.1016/j.jaci.2010.10.013 · DOI · Paper page
  51. Ambruso, 2000, Human neutrophil immunodeficiency syndrome is associated with an inhibitory Rac2 mutation, Proc. Natl. Acad. Sci. USA, 97, 4654, 10.1073/pnas.080074897 · DOI · Paper page
  52. Williams, 2000, Dominant negative mutation of the hematopoietic-specific Rho GTPase, Rac2, is associated with a human phagocyte immunodeficiency, Blood, 96, 1646
  53. Alkhairy, 2015, RAC2 loss-of-function mutation in 2 siblings with characteristics of common variable immunodeficiency, 1380
  54. Li, 2002, Chemoattractant-stimulated Rac activation in wild-type and Rac2-deficient murine neutrophils: preferential activation of Rac2 and Rac2 gene dosage effect on neutrophil functions, J. Immunol., 169, 5043, 10.4049/jimmunol.169.9.5043 · DOI · Paper page
  55. Kim, 2001, Rac2 is an essential regulator of neutrophil nicotinamide adenine dinucleotide phosphate oxidase activation in response to specific signaling pathways, J. Immunol., 166, 1223, 10.4049/jimmunol.166.2.1223 · DOI · Paper page

Cited by

Metadata

Authors
Lu, X., Zhang, Y., Liu, F., & Wang, L.
Venue
The Journal of Immunology
Publisher
Oxford University Press (OUP)
Published
2020-05-01
Volume
204
Issue
9
Pages
2447-2454
ISSN
  • 0022-1767
  • 1550-6606
DOI
10.4049/jimmunol.1901494
Cited by
21
References
4
Language
en
Licence
academic.oup.com/pages/standard-publication-reuse-rights (vor)
Funders
  • Ministry of Science and Technology of China (2018YFA0801200, 2018YFA0800200)
  • National Natural Science Foundation of China (31271570, 31425016, 81530004, 31771604)

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