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Cited by 19Neuroinflammation and Neurodegeneration MechanismsTelomeres, Telomerase, and SenescenceGenetics, Aging, and Longevity in Model Organisms

Late Passage Cultivation Induces Aged Astrocyte Phenotypes in Rat Primary Cultured Cells

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Bang, Minji, Gonzales, Edson Luck, Shin, Chan Young, Kwon, Kyoung Ja·2021·Biomolecules & Therapeutics
DOI 10.4062/biomolther.2020.175PMC PMC7921865
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Abstract

aged astrocyte model through late passage cultivation of rat primary cultured astrocytes. Astrocytes were cultured until passage 7 (P7) as late passage astrocytes and compared with passage 1 (P1) astrocytes as early passage astrocytes to confirm the differences in phenotypes and the effects of serial passage. In this study, we confirmed the morphological, molecular, and functional changes of late passage astrocytes showing aging phenotypes through SA-β-gal staining and measurement of nuclear size. We also observed a reduced expression of inflammatory mediators including IL-1β, IL-6, TNFα, iNOS, and COX2, as well as dysregulation of wound-healing, phagocytosis, and mitochondrial functions such as mitochondrial membrane potential and mitochondrial oxygen consumption rate. Culture-conditioned media obtained from P1 astrocytes promoted neurite outgrowth in immature primary cultures of rat cortices, which is significantly reduced when we treated the immature neurons with the culture media obtained from P7 astrocytes. These results suggest that late passage astrocytes show senescent astrocyte phenotypes with functional defects, which makes it a suitable model for the study of the role of astrocyte senescence on the modulation of normal and pathological brain aging.

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  • After repeated subculture for 8 weeks, astrocytes almost lost their proliferative ability, manifested as over 80% of cells expressing SA-β-Gal.

    “Some astrocytes were repeatedly subcultured for 8 weeks to establish a replicated aging model. At this point, astrocytes almost lost their proliferative ability, manifested as over 80% of cells expressing SA‐β‐Gal [78, 79].”

    From Aerobic exercise improves astrocyte mitochondrial quality and transfer to neurons in a mouse model of Alzheimer's disease (2025) · cites this paper as [78]

    The Methods (Rat primary cortical astrocyte culture; page unavailable) describe repeated subculture to passage 7, and the Results (Late passage astrocytes show phenotypes of morphologically aged cells; page unavailable) report more SA-β-gal-positive cells than at passage 1. Our copy does not establish an eight-week duration, near-total loss of proliferative ability, or SA-β-gal positivity in over 80% of cells; it reports an approximately 1.3-fold increase instead. (LLM, conf=0.87)

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Cited by

Sorted by citations

Authors
Bang, M., Gonzales, E. L., Shin, C. Y., & Kwon, K. J.
Affiliations
Konkuk University (KR)
Corresponding author
Kwon, Kyoung Ja
ORCID
Venue
Biomolecules & Therapeutics
Publisher
The Korean Society of Applied Pharmacology
Published
2021-03-01
Volume
29
Issue
2
Pages
144-153
ISSN
  • 1976-9148 (ISSN-L)
  • 2005-4483
DOI
10.4062/biomolther.2020.175
PMC
PMC7921865
OpenAlex
W3109498668
Cited by
19
References
8
Citations by year
2026: 3 · 2025: 2 · 2024: 3 · 2023: 3 · 2022: 7 · 2021: 1
FWCI
1.05
Type
journal article
Language
en
Open access
Licence
Locations
  • Biomolecules & Therapeutics (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
Konkuk University
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
Keywords
astrocyte senescence, primary astrocyte culture, brain aging, senescence-associated β-galactosidase, inflammatory mediators, mitochondrial dysfunction, mitochondrial membrane potential, mitochondrial oxygen consumption, neurite outgrowth, phagocytosis, wound healing

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