Cited by 29Medicinal Plants and NeuroprotectionMedicinal Plant ResearchPhytochemicals and Medicinal Plants

Comparative study on anti-oxidant and anti-inflammatory activities of Caesalpinia crista and Centella asiatica leaf extracts

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Rao, KS, Ramesh, BN, Girish, TK, Raghavendra, RH, Naidu, KAkhilender, Prasada Rao, U. J. S.·2014·Journal of Pharmacy And Bioallied Sciences

Abstract

BACKGROUND: Amyloidosis, oxidative stress and inflammation have been strongly implicated in neurodegenerative disorders like Alzheimer's disease. Traditionally, Caesalpinia crista and Centella asiatica leaf extracts are used to treat brain related diseases in India. C. crista is used as a mental relaxant drink as well as to treat inflammatory diseases, whereas C. asiatica is reported to be used to enhance memory and to treat dementia. OBJECTIVE: The present study is aimed to understand the anti-oxidant and anti-inflammatory potential of C. asiatica and C. crista leaf extracts. MATERIALS AND METHODS: Phenolic acid composition of the aqueous extracts of C. crista and C. asiatica were separated on a reverse phase C18 column (4.6 x 250 mm) using HPLC system. Antioxidant properties of the leaf extracts were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and the reducing potential assay. The anti-inflammatory activities of aqueous extracts of C. crista and C. asiatica were studied using 5-lipoxygenase assay. Polymorphonuclear leukocytes (PMNLs) were isolated from blood by Ficoll-Histopaque density gradient followed by hypotonic lysis of erythrocytes. RESULTS: Gallic, protocatechuic, gentisic, chlorogenic, caffeic, p-coumaric and ferulic acids were the phenolic acids identified in C. crista and C. asiatica leaf aqueous extracts. However, gallic acid and ferulic acid contents were much higher in C. crista compared to C. asiatica. Leaf extracts of C. asiatica and C. crista exhibited antioxidant properties and inhibited 5-lipoxygenase (anti-inflammatory) in a dose dependent manner. However, leaf extracts of C. crista had better antioxidant and anti-inflammatory activity compared to that of C. asiatica. The better activity of C. crista is attributed to high gallic acid and ferulic acid compared to C. asiatica. CONCLUSIONS: Thus, the leaf extract of C. crista can be a potential therapeutic role for Alzheimer's disease.

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  • Compounds in CA such as ferulic acid and asiatic acid induce Nrf2 and its ARE-regulated targets, exhibiting a neuroprotective effect.

    “Compounds such as the phenolic ferulic acid [89,90] and the triterpene asiatic acid [2,91], induce Nrf2 and its ARE-regulated targets, exhibiting a neuroprotective effect [92,93,94,95].”

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Authors
Rao, K., Ramesh, B., Girish, T., Raghavendra, R., Naidu, K., & Prasada Rao, U. J. S.
Affiliations
  • Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (PA)
  • Central Food Technological Research Institute (IN)
Corresponding author
Rao, KS
ORCID
Rao, KS
Venue
Journal of Pharmacy And Bioallied Sciences
Published
2014
Volume
6
Issue
2
Pages
86
ISSN
0975-7406 (ISSN-L)
OpenAlex
W2110723235
Cited by
26
References
34
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 2 · 2019: 4 · 2018: 3 · 2017: 3 · 2016: 2 · 2015: 1
FWCI
4.30
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • Journal of Pharmacy And Bioallied Sciences (publishedVersion, cc-by-nc-sa)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
  • Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
Keywords
Caesalpinia crista, Centella asiatica, leaf extract, antioxidant activity, anti-inflammatory activity, phenolic acids, gallic acid, ferulic acid, 5-lipoxygenase inhibition, DPPH radical scavenging, Alzheimer's disease, oxidative stress, neurodegenerative diseases, traditional medicine

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