Therapeutic effects of Cyathula officinalis Kuan and its active fraction on acute blood stasis rat model and identification constituents by HPLC-QTOF/MS/MS
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BACKGROUND: Kuan is widely used in the clinics for the treatment of blood stasis in China. OBJECTIVE: Kuan extract (CO) and its active fraction (ACO) on acute blood stasis model Wistar rats and characterize the correlative constituents. MATERIALS AND METHODS: CO at 0.26, 0.53, and 1.04 g/kg and ACO at 0.38, 0.75, and 1.5 g/kg were administered to acute blood stasis model Wistar rats for 3 days. Whole blood viscosity, plasma viscosity, and the levels of interleukin-6 (IL-6), nitric oxide (NO), tumor necrosis factor alpha (TNF-α), and cyclooxygenase-2 (COX-2) in the plasma were measured. HPLC-QTOF/MS/MS method was used to identify the major constituents of ACO; the properties of two representative components (cyasterone and chikusetsusaponin IV) from ACO on thrombin-induced human umbilical vein endothelial cells damage model were also assessed by the levels of thromboxane A2 (TXA2), endothelin (ET), malondialdehyde (MDA), COX-2, endothelial nitric oxide synthase (eNOS), and superoxide dismutase (SOD). RESULTS: . CONCLUSION: . SUMMARY: Kuan, ROS: Reactive oxygen species, IL-6: Interleukin-6, TNF-α: Tumor necrosis factor alpha, NO: Nitric oxide, COX-2: Cyclooxygenase-2, TXA2: Thromboxane A2, ET: Endothelin, MDA: Malondialdehyde, eNOS: Endothelial nitric oxide synthase, SOD: Superoxide dismutase, ESI: Electronic spray ionization, ELISA: Enzyme-linked immunosorbent assay, HUVECs: Human umbilical vein endothelial cells, DMEM: Dulbecco's modified Eagle medium, MMP: Matrix metalloproteinase.
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Cyasterone along with chikusetsusaponin IV significantly inhibited inflammatory and oxidative enzyme levels, including TXA2, ET, MDA, and COX-2, and promoted eNOS and SOD activities.
“Another PE derivative cyasterone along with chikusetsusaponin IV could significantly inhibit inflammatory and oxidative enzyme levels, such as thromboxane A2 (TXA2), endothelin (ET), malondialdehyde (MDA), and cyclooxygenase-2 (COX-2) as well as promote the activities of endothelial nitric oxide synthase (eNOS) and superoxide dismutase (SOD)154.”
From The Phytochemical, Biological, and Medicinal Attributes of Phytoecdysteroids: An Updated Review (2021) · cites this paper as [154]
PE derivatives inhibit inflammatory and oxidative enzyme activity, such as thromboxane A2, malondialdehyde (MDA), endothelin, and cyclooxygenase-2, which increases activity of antioxidant enzymes.
“PE derivatives inhibit inflammatory and oxidative enzyme activity, such as thromboxane A2, malondialdehyde (MDA), endothelin, and cyclooxygenase-2, which increases the activity of antioxidant enzymes [158].”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [158]
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- Authors
- Yin, L., Cao, Y., Gu, C., Zhao, F., Tang, Y., Cui, X., Shi, L., & Xu, L.
- Affiliations
- Nanjing University of Chinese Medicine (CN)
- Corresponding author
- Yin, Lian
- Venue
- Pharmacognosy Magazine
- Publisher
- SAGE Publications
- Published
- 2017
- Volume
- 13
- Issue
- 52
- Pages
- 693
- ISSN
- 0973-1296 (ISSN-L)
- PMC
- PMC5701413
- OpenAlex
- W2768343935
- Cited by
- 24
- References
- 42
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 6 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 1
- FWCI
- 2.03
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- Pharmacognosy Magazine (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Keywords
- Cyathula officinalis, blood stasis, active fraction, cyasterone, endothelial dysfunction, oxidative stress, inflammatory cytokines, cyclooxygenase-2, thromboxane A2, endothelin, blood viscosity, traditional Chinese medicine
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