Cited by 24Traditional Chinese Medicine AnalysisPhytochemistry and Biological ActivitiesMedicinal Plant Research

Therapeutic effects of Cyathula officinalis Kuan and its active fraction on acute blood stasis rat model and identification constituents by HPLC-QTOF/MS/MS

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Yin, Lian, Cao, Yanmei, Gu, Cuicui, Zhao, Fangli, Tang, Yuanlin, Cui, Xiaobing, Shi, Le, Xu, Li·2017·Pharmacognosy Magazine

Abstract

BACKGROUND: Kuan is widely used in the clinics for the treatment of blood stasis in China. OBJECTIVE: Kuan extract (CO) and its active fraction (ACO) on acute blood stasis model Wistar rats and characterize the correlative constituents. MATERIALS AND METHODS: CO at 0.26, 0.53, and 1.04 g/kg and ACO at 0.38, 0.75, and 1.5 g/kg were administered to acute blood stasis model Wistar rats for 3 days. Whole blood viscosity, plasma viscosity, and the levels of interleukin-6 (IL-6), nitric oxide (NO), tumor necrosis factor alpha (TNF-α), and cyclooxygenase-2 (COX-2) in the plasma were measured. HPLC-QTOF/MS/MS method was used to identify the major constituents of ACO; the properties of two representative components (cyasterone and chikusetsusaponin IV) from ACO on thrombin-induced human umbilical vein endothelial cells damage model were also assessed by the levels of thromboxane A2 (TXA2), endothelin (ET), malondialdehyde (MDA), COX-2, endothelial nitric oxide synthase (eNOS), and superoxide dismutase (SOD). RESULTS: . CONCLUSION: . SUMMARY: Kuan, ROS: Reactive oxygen species, IL-6: Interleukin-6, TNF-α: Tumor necrosis factor alpha, NO: Nitric oxide, COX-2: Cyclooxygenase-2, TXA2: Thromboxane A2, ET: Endothelin, MDA: Malondialdehyde, eNOS: Endothelial nitric oxide synthase, SOD: Superoxide dismutase, ESI: Electronic spray ionization, ELISA: Enzyme-linked immunosorbent assay, HUVECs: Human umbilical vein endothelial cells, DMEM: Dulbecco's modified Eagle medium, MMP: Matrix metalloproteinase.

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  • Cyasterone along with chikusetsusaponin IV significantly inhibited inflammatory and oxidative enzyme levels, including TXA2, ET, MDA, and COX-2, and promoted eNOS and SOD activities.

    “Another PE derivative cyasterone along with chikusetsusaponin IV could significantly inhibit inflammatory and oxidative enzyme levels, such as thromboxane A2 (TXA2), endothelin (ET), malondialdehyde (MDA), and cyclooxygenase-2 (COX-2) as well as promote the activities of endothelial nitric oxide synthase (eNOS) and superoxide dismutase (SOD)154.”
  • PE derivatives inhibit inflammatory and oxidative enzyme activity, such as thromboxane A2, malondialdehyde (MDA), endothelin, and cyclooxygenase-2, which increases activity of antioxidant enzymes.

    “PE derivatives inhibit inflammatory and oxidative enzyme activity, such as thromboxane A2, malondialdehyde (MDA), endothelin, and cyclooxygenase-2, which increases the activity of antioxidant enzymes [158].”

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  1. [1]Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China 2010 Beijing China Medical Science Press 35 6

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Authors
Yin, L., Cao, Y., Gu, C., Zhao, F., Tang, Y., Cui, X., Shi, L., & Xu, L.
Affiliations
Nanjing University of Chinese Medicine (CN)
Corresponding author
Yin, Lian
Venue
Pharmacognosy Magazine
Published
2017
Volume
13
Issue
52
Pages
693
ISSN
0973-1296 (ISSN-L)
OpenAlex
W2768343935
Cited by
24
References
42
Citations by year
2026: 1 · 2025: 4 · 2024: 6 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 1
FWCI
2.03
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • Pharmacognosy Magazine (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
Keywords
Cyathula officinalis, blood stasis, active fraction, cyasterone, endothelial dysfunction, oxidative stress, inflammatory cytokines, cyclooxygenase-2, thromboxane A2, endothelin, blood viscosity, traditional Chinese medicine

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